A5017756551- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Immune Cell Function and Interaction
- IL-33, ST2, and ILC Pathways
- Dermatology and Skin Diseases
- Helicobacter pylori-related gastroenterology studies
- Microbial Metabolites in Food Biotechnology
- Microscopic Colitis
- Probiotics and Fermented Foods
- Neonatal Respiratory Health Research
- Animal health and immunology
- Infant Nutrition and Health
- Reproductive System and Pregnancy
- Viral gastroenteritis research and epidemiology
- Dietary Effects on Health
- Diet, Metabolism, and Disease
- Pediatric health and respiratory diseases
University Medical Center Groningen
2017-2020
University of Groningen
2017-2020
TiFN
2016-2019
Abstract Aging significantly increases the vulnerability to gastrointestinal (GI) disorders but there are few studies investigating key factors in aging that affect GI tract. To address this knowledge gap, we used 10-week- and 19-month-old litter-mate mice investigate microbiota host gene expression changes association with ageing. In aged thickness of colonic mucus layer was reduced about 6-fold relative young mice, more easily penetrable by luminal bacteria. This linked increased apoptosis...
A mucus layer covers and protects the intestinal epithelial cells from direct contact with microbes. This not only prevents inflammation but also plays an essential role in microbiota colonization, indicating complex interplay between composition-microbiota health. However, it is unknown whether influenced by age or sex this contributes to reported differences diseases males females ageing. Therefore, study we investigated effect of on thickness, composition immune relation sex. The ageing...
A dysbiosis in the intestinal microbiome plays a role pathogenesis of several immunological diseases. These diseases often show sex bias, suggesting differences immune responses and microbiome. We hypothesized that are associated with microbiota composition. Fecal composition (MITchip), mRNA expression tissue (microarray), cell populations mesenteric lymph nodes (MLNs) were studied male female mice two mouse strains (C57B1/6OlaHsd Balb/cOlaHsd). Transcriptomics data combined to identify...
Scope The epithelial glycocalyx development is of great importance for microbial colonization. Human milk oligosaccharides (hMOs) and non‐digestible carbohydrates (NDCs) may modulate development. Methods results effects hMOs NDCs on human gut cells (Caco2) are investigated by quantifying thickness area coverage adsorbed albumin, heparan sulfate (HS), hyaluronic acid (HA) in the glycocalyx. Effects (2′‐FL 3‐FL) [inulins with degrees polymerization (DP) (DP3‐DP10, DP10‐DP60, DP30‐DP60) pectins...
It has been shown in vitro that only specific dietary fibers contribute to immunity, but studies vivo are not conclusive. Here, we investigated degree of polymerization (DP) dependent effects β2→1-fructans on immunity via microbiota-dependent and -independent effects. To this end, conventional or germ-free mice received short- long-chain β2→1-fructan for 5 days. Immune cell populations the spleen, mesenteric lymph nodes (MLNs), Peyer's patches (PPs) were analyzed with flow cytometry,...
During ageing, dysbiosis in the intestinal microbiota may occur and impact health. There is a paucity of studies on effect fiber elderly flexibility aged upon prebiotic intake. It hypothesized that chicory long-chain inulin consumption can change composition, microbial fermentation products, immunity elderly.
In vivo studies demonstrating that only specific dietary-fibers contribute to immunity are still inconclusive, as measuring immune effects in healthy humans remains difficult. We applied a relatively inefficacious vaccination-challenge study chain length-dependent of inulin-type fructan (ITF) dietary fibers on human immunity.ITFs with two different 'degree polymerization-' (DP)-profiles were tested vitro for PBMC-cytokines and TLR2 activation. double-blind placebo-controlled trial, 40...
Males and females have a different predisposition for the development of intestinal disorders, like inflammatory bowel disease (IBD). We hypothesized that sex specific differences in immune responses may underlie this bias. To test hypothesis, we studied cell populations Peyer's patches (PP). For comparison with systemic immunity, spleen cells.Two mouse strains susceptibility developing colitis (BALB/c C57Bl/6) were used. Using flow cytometry, measured percentage T cells, Th1, Th17, Treg...
Hormones and placental factors are thought to underlie the maternal immunological changes during pregnancy. However, as several intestinal microbiota linked immune modulations, we hypothesized that altered pregnancy in favor of species associated with modulations. We studied fecal composition (MITchip) peripheral cells (microarray flow cytometry) pregnant non-pregnant C57BL/6 BALB/c mice. Pregnancy influenced diversity composition, however a mouse strain dependent way. Pregnant mice had,...