Summary Gene silencing, or RNA interference, is a powerful tool for elucidating gene function in Caenorhabditis elegans and Drosophila melanogaster . The vast genetic, developmental sequence information available Arabidopsis thaliana makes this an attractive organism which to develop reliable gene‐silencing tools the plant world. We have developed system based on bipartite geminivirus cabbage leaf curl virus (CbLCV) that allows silencing of endogenous genes singly combinations Two vectors...

Geminiviruses are DNA viruses that replicate and transcribe their genes in plant nuclei. They ideal vectors for understanding gene function because of ability to cause systemic silencing new growth ease inoculation. We previously demonstrated episome-mediated from a bipartite geminivirus Nicotiana benthamiana. Using an improved vector, we now show extensive endogenous can be obtained using less than 100 bp homologous sequence. Concomitant symptom development varied depending upon the target...

Summary Virus‐induced gene silencing (VIGS) is a sequence‐specific RNA degradation process that can be used to downregulate plant expression. Both and DNA viruses have been for VIGS, but they differ in their mode of replication, expression, cellular location. This study examined mediated by virus, cabbage leaf curl virus (CaLCuV), several silencing‐deficient Arabidopsis mutants. A VIGS vector derived from CaLCuV, which silenced chlorata42 ( ChlI ) needed chlorophyll formation, was test...

Kaempferia larsenii Sirirugsa, a rare species in Thailand belonging to the Zingiberaceae family, is known for its effective pharmaceutical properties. However, slow natural growth and threats from overharvesting habitat encroachment pose significant challenges. This study investigated effects of plant regulators (PGRs) on regeneration, transplantation success, phytochemical profiling, antioxidant properties wild vitro-cultured plants. Plantlets (~1 cm long) were cultivated 8 weeks different...

Both RNA and DNA viruses have been engineered to serve as vectors for transient silencing in intact plants. Host gene sequences carried by the virus are seen plant "foreign," homologous gene-silencing machinery acts on both viral vector endogenous host mRNA. viruses, such geminiviruses, advantageous because only their mRNAs silenced genomes continue replicate move. The conserved genome organization of geminiviruses fact that they can be cloned into Escherichia coli plasmids, propagated, then...

Abstract One new species and six records of the crustose lichenized genus Graphis are reported from Thailand. koratensis Pitakpong, Kraichak & Lücking sp. nov. is characterized by lirelline ascocarps with whitish grey or grey-green pruina along slit, transversely septate ascospores, presence norstictic acid. Phylogenetic analyses two loci (mtSSU nuLSU) show distinct position this within genus. Six for Thailand reported, including G. cincta (Pers.) Aptroot, jejuensis K. H. Moon et al. ,...

Globba (Zingiberaceae) are attractive herbaceous plants widely used as ornamental plants. However, cultivation is limited because of low propagation rate and depends on the season only. This research aimed to compare growth physiological responses schomburgkii Hook. f. marantina L. under both soil hydroponic conditions. In vitro plantlets (8 cm in height) were transplanted a culture with nutrient film technique (NFT). culture, small pots containing sand: burned rice husk: peat moss (1:1:1 by...

Abstract. Muangsan N, Saensouk P, Chanokkhun T, Watthana S. 2022. Comparative leaf epidermis study in Habenaria spp. (Orchidaceae) from Thailand. Biodiversitas 23: 4159-4168. The variation of epidermal and stomatal characteristics could be used for plant classification, including orchids. This aimed to investigate characters Thailand focused on the H. rhodocheila group related species. Fourteen accessions belonging 10 species were studied compared. Epidermal peels taken upper lower surfaces...

Abstract Aptrootia khaoyaiensis Polyiam & Lücking is described as new to science from dry dipterocarp forest in central Thailand, based on morphological assessment and sequence data of the mitochondrial small subunit (mtSSU). The species characterized by a corticolous habit, corticate thallus, ascomata typically immersed with brown blackish ostiolar region, an inspersed hamathecium, dark brown, muriform ascospores occurring 1–2 per ascus. Phylogenetic analyses support placement ; it...

Five edible species of the genus Kaempferia—K. minuta, K. phuphanensis, sisaketensis, takensis, and udonensis—in Thailand were cytologically studied by their root tips. The somatic chromosome numbers all found to be 2n = 22, FNs revealed 44. karyotype five rare endemic was provided: 10m + 12sm with three satellites for 12m 10sm six 18m 4sm four 6m 6st 14m 2sm two udonensis. This research identified new karyological information regarding number, FN, karyotype, ideogram species. They had a...

Curcuma spaganifolia Gagnep., belonging to the family Zingiberaceae, has attracted widespread attention due its considerable economic importance.The conventional propagation method for ginger takes a long time multiplication low rate and poor seed germination, therefore, tissue culture is an important manipulation of plants higher propagation.The present study deals with in vitro ex conservation C. rare plant species from Thailand.Murashige Skoog (MS) media different concentrations growth...

A new species, Maerua koratensis (Capparaceae), only known from a restricted locality in Nakhon Ratchasima Province, eastern Thailand, is here described and illustrated. This the second species of to be South-East Asia. distinguished by simple leaves, petaliferous flowers with distinctly 4-lobed laciniate disc, cylindrical, torulose moniliform fruits. Morphological characteristics M. juncea subsp. juncea, its most similar, are compared. key occurring mainland Asia provided.

The genus Micropera Lindl.(Orchidaceae: Aeridinae) is revised for Thailand based on herbarium specimens, a literature review, and field observations.Six taxa are recognized, including two new country records.