A robust method, for the chromatographic separation of three antidiabetic drugs viz metformin, pioglitazone and glimepiride using an isocratic reversed phase high-performance liquid (HPLC) system having ultraviolet detection at 254 nm is presented in this paper. The method was developed human serum dosage formulation with high-quality between drug peaks by a stainless steel analytical column Nucleosil, C18 (10 micron, 25 × 0.46 cm). operated room temperature mobile consisting acetonitrile,...

Abstract Co‐administration of HMG‐CoA reductase with antidiabetic drugs is most common since are mostly prescribed for long term therapy. In the present paper, we describe simultaneous determination (pioglitazone hydrochloride and gliquidone) in presence statins (rosvastatin simvastatin) formulations human serum using RP‐HPLC technique. The samples were subjected to protein precipitation acetonitrile prior an HPLC analysis. At a flow rate 1 mL·min −1 isocratic elution was employed, mobile...

A series of phosphonated carbocyclic 2 ′ ‐oxa‐3 ‐aza‐nucleosides were synthesized via 1,3 dipolar cycloaddition and evaluated for their in vitro antiproliferative activity against the growth cancer cell lines ( MCF ‐7, A2780, HCT 116) normal non‐transformed fibroblast MRC 5) using MTT assay. Synthesized compounds exhibited micromolar range. Compounds 11b showed highest ‐7 cells IC 50 0.2344 μ m ). Cell cycle analysis was performed compound on 7 showing arrest S phase. Molecular docking...

Tremendous research from last twenty years has been pursued to cure human life against HIV virus. A large number of protease inhibitors are in clinical trials but still it is an interesting target for researchers due the viral ability get mutated. Mutated strains led drug ineffective used increase span patients. In present work, 3D-QSAR and docking studies were performed on a series Danuravir derivatives, most potent HIV- inhibitor known so far. Combined study was applied derivatives using...

Cetirizine (anti-allergy agent) and non-steroidal anti-inflammatory drugs (NSAIDs; agents) are co-administered drugs. Cetirizine, a P-glycopretein substrate may be affected by the use of NSAIDs. In present work, quantification cetirizine in presence commonly NSAIDs such as diclofenac sodium, ibuprofen, flurbiprofen, tiaprofenac acid, meloxicam mefenamic acid were studied using RP-HPLC technique. A high-throughput HPLC method for analysis was developed, validated further used to study...

A simple, accurate and precise RP-HPLC method was developed for the simultaneous determination of chloroquine, pyrimethamine cetirizine hydrochloride concentrations in bulk drug human serum. The assay performed using a mobile phase methanol: water (70:30) at pH 2.8 ± 0.05 on Purospher C-18 column with UV detection 230 nm rosuvastatin used as an internal standard. retention times observed were 3.5, 2.5 5.5 minutes, respectively. found to be specific assayed drugs showing linear response...

Simple and sensitive spectrophotometric method is described based on the reaction of drug (gabapentin) with ninhydrin in pure form pharmaceutical preparations. Complex formed during this measured at 575 nm as a function time. Kinetic study involve initial-rate, rate-constant fixed-time (80 minutes) procedures to determine concentration drug. Drug was studied range 10-30 ?gmL-1 showing correlation coefficient 0.9997, 0.9970 0.9990 for initial rate, rate constant fixed time respectively. Limit...

Gold nanorods have received immense importance due to their potential for strong optical absorption in the visible and near infrared regions. A new approach was employed develop a stable probe based on an anionic polyelectrolyte, poly(sodium-4-styrenesulfonate) PSS, novel, non-cytotoxic antimicrobial contraceptive agent, functionalized gold nanorods, followed by attachment of anti-cancer drug, doxorubicin. Doxorubicin has proved be one potent anticancer drugs, establishing wide applicability...

High Performance Liquid Chromatographic method was developed and applied for the simultaneous determination of diltiazem fluoroquinolones in bulk, pharmaceutical formulations human serum presence caffeine as internal standard.A Hiber®, RP-18 column used with mobile phase consisting acetonitrile:methanol:water (30:20:50 v/v, pH 3.6) quantitative evaluation performed at 230 nm.Suitability this drugs proved by validation accordance requirements laid down International Conference on...

A simple, sensitive, and rapid RP-HPLC method for analysis of enalapril in the presence H2-receptor antagonists has been developed validated. Enalapril maleate was separated from by use a 250 mm × 4.6 mm, 5-µm particle, C18 column with 86:14 (υ/υ) methanol-water, pH adjusted to 3.5, as mobile phase, at flow rate 1.5 mL min−1. UV detection performed 227 nm. The retention times maleate, ranitidine, cimetidine, famotidine were 3, 5, 7, 7.5 min, respectively. limit 10 ng mL−1 calibration plot...

Present work was designed for the simultaneous determination of cetirizine in presence atorvastatin, simvastatin, rosuvastatin or pravastatin using RP-HPLC method. The chromatographic separation achieved mobile phase consisted acetonitrile: water (75:25), monitored at 235 nm with a pH adjusted to 2.8. linearity assessed over range 2.5-100 µgmL-1 all drugs. applicability method verified by cetirizine, and marketed formulations. results were validated statistically according ICH guidelines....

Background: Human African trypanosomiasis is a fatal disease prevalent in approximately 36 sub-Saharan countries. Emerging reports of drug resistance Trypanosoma brucei are serious cause concern as only limited drugs available for the treatment disease. Pteridine reductase an enzyme brucei. Methods: It plays critical role pterin metabolic pathway that absolutely essential its survival human host. The success finding potent inhibitor structure-based design lies within ability computational...

7 Abstract: Multivariate calibration technique using spectrophotometer is underway to assess quantitatively mebeverine in intermediate and fished stage of different dosage forms pharmaceutical. This approach bases on the use linear regression analysis equation by correlation concentration drugs corresponding absorbance taken, at five unlike wavelengths. Statistical evaluation results finds method be highly accurate, precise reproducible. Drug molecule was studied range 5-80 µg/mL...

Malaria causes about half a million deaths per year, mainly in children below 5 years of age. Cytoadherence Plasmodium falciparum infected erythrocytes brain and placenta has been linked to severe malaria malarial related deaths. is mediated by binding human receptor gC1qR the DBLβ12 domain P. erythrocyte membrane protein family 1 (PfEMP1) protein. In present work, molecular dynamic simulation was extensively studied for gC1qR-DBLβ12 complex. The stabilized complex used study protein-protein...

Determination of cetirizine, diltiazem, or verapamil and amlodipine in an active dosage formulations has been performed using a simple RP-HPLC method. Rosuvastatin is used this novel method as internal standard to improve the selectivity At 230 nm, separation was mobile phase consisting methanol, acetonitrile, water mixture ratio 65:5:30, pH at 2.8 allowed improved faster times analysis. ICH guidelines have pursued validation by assessing accuracy, precision (%RSD>2), linearity (>0.999). The...