A5073229588- Adipokines, Inflammation, and Metabolic Diseases
- Cytokine Signaling Pathways and Interactions
- DNA Repair Mechanisms
- Cancer-related molecular mechanisms research
- Kruppel-like factors research
- Folate and B Vitamins Research
- Cancer, Lipids, and Metabolism
- Carcinogens and Genotoxicity Assessment
- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- Genetic factors in colorectal cancer
- Autophagy in Disease and Therapy
- Monoclonal and Polyclonal Antibodies Research
- Cancer-related Molecular Pathways
- Regulation of Appetite and Obesity
- Head and Neck Cancer Studies
- Oral Health Pathology and Treatment
- Cancer Cells and Metastasis
- Traditional and Medicinal Uses of Annonaceae
- FOXO transcription factor regulation
- Adipose Tissue and Metabolism
- MicroRNA in disease regulation
- Histone Deacetylase Inhibitors Research
- Pregnancy and preeclampsia studies
- Protease and Inhibitor Mechanisms
Kaohsiung Medical University
2016-2025
Kaohsiung Medical University Chung-Ho Memorial Hospital
2016-2025
National Yang Ming Chiao Tung University
2019-2024
Nanjing Medical University
2024
I-Shou University
2006-2023
E-Da Hospital
2005-2023
Zhongshan Hospital of Xiamen University
2022-2023
Translational Research Institute
2015
Taipei Medical University
2015
Wan Fang Hospital
2015
Genetic studies in yeast have indicated a role of the RAD50 and MRE11 genes homologous recombination, telomere length maintenance, DNA repair processes. Here, we purify from nuclear extract Raji cells complex consisting human Rad50, Mre11, another protein factor with size about 95 kDa (p95), which is likely to be Nibrin, encoded by gene mutated Nijmegen breakage syndrome. We show that Rad50-Mre11-p95 possesses manganese-dependent single-stranded endonuclease 3′ 5′ exonuclease activities....
To assess the functions of retinoblastoma protein (RB) during normal development, we have analyzed mouse embryos that lack a functional copy gene (genotype: Rb-1 delta 20/Rb-1 20). Our findings demonstrate RB plays an important role in regulation neuronal cell cycle. In mutant embryos, dividing cells are found well outside neurogenic regions both central and peripheral nervous systems. addition to abnormal cycle regulation, however, show two less expected phenotypes. First, many ectopically...
Cells from individuals with the recessive cancer-prone disorder ataxia telangiectasia (A-T) are hypersensitive to ionizing radiation (I-R). ATM (mutated in A-T) is a protein kinase whose activity stimulated by I-R. c-Abl, nonreceptor tyrosine kinase, interacts and activated following Rad51 homologue of bacterial RecA required for DNA recombination repair. Here we demonstrate that there an I-R-induced phosphorylation, this induction dependent on both c-Abl. ATM, can be co-immunoprecipitated...
To investigate the entire process of autophagy in left ventricle septic mice, and functional significance by using pharmacological agents.Myocardial dysfunction is a common feature sepsis contributes to an increased risk developing multiple organ failure. Autophagy functions predominantly as prosurvival pathway heart during cellular stress. A dynamic that involves complete activation from autophagosome formation fusion with lysosomes has driven development new approaches detecting...
To clarify the role of autophagy in sepsis-induced lung injury.The as a protective or maladaptive response cells during sepsis has not yet been determined. The lack specificity autophagic process driven development new approaches that assess autophagosomes from formation to fusion with lysosomes.Sepsis was induced by cecal ligation and puncture (CLP). manipulated using pharmacological inhibitors pathway. Green fluorescent protein (GFP)-microtubule-associated 1 light chain 3 (LC3) transgenic...
Abstract Background: Adipocytokines, adipocyte-secreted hormones, play a critical role in breast cancer development. The expression of visfatin, newly discovered adipocytokine, tissues was determined and correlated with patient clinicopathologic variables. Methods: Visfatin analyzed by immunohistochemistry. variables as well recurrence rates, using the χ2 test. prognostic value visfatin for disease-free overall survival evaluated Kaplan–Meier estimates, significance differences between...
Previous studies have shown that altered forms of MRE11, a protein known to play vital role in DNA double-strand break repair, replication, and telomere maintenance are associated with cancer outcomes. We investigated the MRE11 breast both clinical vitro settings. examined expression tumor tissues from invasive ductal carcinoma patients (n = 254) by immunohistochemistry, associations clinicopathological characteristics overall survival were assessed using Cox proportional hazards regression...
The accumulation of autophagosomes in the terminal step autophagic process has recently emerged as a potentially maladaptive septic heart and lung. However, role autophagy liver not been ascertained. This study was investigated by first examining entire sequence mice. Second, novel pharmacotherapeutic approach utilized to treat sepsis with enhancer/inhibitor. Sepsis induced cecal ligation puncture (CLP). C57BL/6 mice received enhancer carbamazepine (CBZ), inhibitor 3-methyladenine...
Visfatin is an adipocytokine involved in cellular metabolism, inflammation, and cancer. This study investigated the roles of extracellular visfatin breast cancer, explored underlying mechanisms clinical experimental settings.Associations serum with clinicopathologic characteristics patient survival were assessed Cox regression models Kaplan-Meier analyses. Effects on cultured cancer cells examined, followed by vivo investigation tumor growth metastasis xenograft animal models. Imatinib...
Background Although the role of autophagy in sepsis has been characterized several organs, its adaptive immune system remains to be ascertained. This study aimed investigate sepsis-induced T cell apoptosis and immunosuppression, using knockout mice with specific deletion essential gene Atg7. Methods Results Sepsis was induced a cecal ligation puncture (CLP) model, T-cell-specific Atg7-knockout compared control mice. Autophagic vacuoles examined by electron microscopy were decreased spleen...
Nitric oxide (NO) is an important cellular signaling molecule that modulates various physiological activities. Angiogenesis-promoting activities of NO-donor drugs have been explored in both experimental and clinical studies. In this study, a structurally well characterized water-soluble neutral {Fe(NO)2}(9) DNIC [(S(CH2)2OH)(S(CH2)2NH3)Fe(NO)2] (DNIC 2) was synthesized to serve as species. The antitumor activity 2 determined by MTT assay, confocal imaging, Annexin-V/PI staining. IC50 values...