A5064887735- Advanced biosensing and bioanalysis techniques
- Advanced Biosensing Techniques and Applications
- Molecular Junctions and Nanostructures
- Spectroscopy and Quantum Chemical Studies
- Plasmonic and Surface Plasmon Research
- Gold and Silver Nanoparticles Synthesis and Applications
- Electrochemical Analysis and Applications
- Analytical Chemistry and Sensors
- Biosensors and Analytical Detection
- Monoclonal and Polyclonal Antibodies Research
- DNA and Biological Computing
- Photochemistry and Electron Transfer Studies
- Polymer Surface Interaction Studies
- Nanofabrication and Lithography Techniques
- DNA and Nucleic Acid Chemistry
- Photonic and Optical Devices
- RNA Interference and Gene Delivery
- Photonic Crystals and Applications
- Gene expression and cancer classification
- Diatoms and Algae Research
- Microfluidic and Bio-sensing Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Bacteriophages and microbial interactions
- Force Microscopy Techniques and Applications
- Nanoparticle-Based Drug Delivery
University of California, Irvine
2012-2024
University of Wisconsin–Madison
1996-2008
University of California System
2008
Irvine University
2008
Newport (United States)
2007
Intrinsic LifeSciences (United States)
2006
Texas A&M University
2003
Kyoto University
2002
Science and Technology Corporation (United States)
2002
University of British Columbia
2002
Surface plasmon resonance (SPR) imaging is a surface-sensitive spectroscopic technique for measuring interactions between unlabeled biological molecules with arrays of surface-bound species. In this paper, SPR used to quantitatively detect the hybridization adsorption short (18-base) DNA oligonucleotides at low concentration, as well as, first time, RNA and larger 16S ribosomal (rRNA) isolated from microbe Escherichia coli onto array. For both oligonucleotides, detection limit 10 nM...
The surface-sensitive optical technique of surface plasmon resonance (SPR) imaging is used to characterize ultrathin organic and biopolymer films at metal interfaces in a spatially resolved manner. Because its high sensitivity ability measure real time the interaction unlabeled biological molecules with arrays surface-bound species, SPR has potential become powerful tool biomolecular investigations. Recently, been successfully implemented characterization supported lipid bilayer films,...
Carbohydrate arrays fabricated on gold films were used to study carbohydrate-protein interactions with surface plasmon resonance (SPR) imaging. An immobilization scheme consisting of the formation a disulfide bond was attach thiol-modified carbohydrates onto and fabricate carbohydrate arrays. The attachment steps characterized using polarization modulation Fourier transform infrared reflection absorption spectroscopy; poly(dimethylsiloxane) microchannels immobilize probe compounds at...
Multiple microRNAs (miRNAs) are detected in a microarray format using novel approach that combines surface enzyme reaction with nanoparticle-amplified SPR imaging (SPRI). The of poly(A) polymerase creates tails on miRNAs hybridized onto locked nucleic acid (LNA) microarrays. DNA-modified nanoparticles then adsorbed the and SPRI. This ultrasensitive SPRI methodology can be used for miRNA profiling at attomole levels.
A multistep surface modification procedure for the creation of DNA arrays on chemically modified gold surfaces that can be used in plasmon resonance (SPR) imaging studies protein−DNA interactions is demonstrated. The required to create an array spots are surrounded first by a hydrophobic background which allows pinning aqueous solutions onto individual elements and then replace with one resists nonspecific adsorption proteins during situ SPR measurements. An amine-terminated alkanethiol...
A monolayer of poly(l-lysine) (PL) is attached covalently via amide bonds to an alkanethiol self-assembled (SAM) on a gold surface. The bond formed in two steps: the terminal carboxylic acid groups 11-mercaptoundecanoic (MUA) SAM are first activated N-hydroxysulfosuccinimide (NHSS) ester, followed by reaction this MUA-NHSS ester with amino PL create multiple linkages reactivity and packing density esters investigated detail reacting these intermediates ammonia (NH3). In NH3 experiments,...
A combination of scanning and imaging surface plasmon resonance (SPR) experiments is used to characterize DNA hybridization adsorption at gold surfaces the subsequent immobilization streptavidin. Single-stranded oligonucleotides are immobilized surfaces, biotinylated complements from solution monitored with SPR. The attachment streptavidin provides a method enhancing SPR signal produced as result leads 4-fold improvement in detection limit apparatus. In situ measure 60 ± 20% efficiency...
A methodology for the detection of protein biomarkers at picomolar concentrations that utilizes surface plasmon resonance imaging (SPRI) measurements RNA aptamer microarrays is developed. The adsorption proteins onto microarray detected by formation a aptamer−protein−antibody complex. SPRI response signal then amplified using localized precipitation reaction catalyzed enzyme horseradish peroxidase conjugated to antibody. This enzymatically first characterized human thrombin concentration 500...
A new method for constructing oligonucleotide arrays on gold surfaces has been developed, and these have used in DNA hybridization experiments with situ surface plasmon resonance (SPR) imaging detection. The detection technique was able to differentiate between single- double-stranded regions the surface. of both oligonucleotides PCR-amplified fragments detectable, latter exhibiting slower kinetics. Temperature control SPR cell discriminate perfectly matched duplexes single-base-mismatched...
The characterization of peptide arrays on gold surfaces designed for the study peptide−antibody interactions using surface plasmon resonance (SPR) imaging is described. A two-step process was used to prepare arrays: (i) a set parallel microchannels deliver chemical reagents covalently attach probes by thiol−disulfide exchange reaction; (ii) second microchannel with wraparound design as small-volume flow cell (5 μL) introduce antibody solutions surface. As demonstration, FLAG epitope tag and...
A novel bioaffinity sensor based on surface plasmon resonance (SPR) imaging measurements of a multiple-layered structure that supports the generation long-range plasmons (LRSPs) at water−metal interface is reported. LRSPs possess longer propagation lengths, higher electric field strengths, and sharper angular curves than conventional plasmons. LRSPR version SPR requires symmetric dielectric arrangement around gold thin film. This created using an SF10 prism/Cytop/gold/water multilayer film...
The application of surface plasmon resonance (SPR) measurements to the study ultrathin organic and inorganic films adsorbed onto gold surfaces utilizing near-infrared (NIR) excitation from 800 1152 nm is described. SPR scanning angle film thickness are demonstrated at 814 using low-power diode HeNe laser sources, respectively. Several advantages in NIR noted. situ reflectivity versus incidence curves sharpen greatly (as compared 632.8 nm) longer wavelengths so that there no loss sensitivity...
We consider the problem of designing DNA codes, namely sets equi-length words over alphabet [A, C, G, T] that satisfy certain combinatorial constraints. This is motivated by task reliably storing and retrieving information in synthetic strands for use computing or as molecular bar codes chemical libraries. The primary constraints we consider, defined with respect to a parameter d, are follows: every pair w, x code, there at least d mismatches between w if not equal also reverse Watson-Crick...
A combination of in situ and ex surface plasmon resonance (SPR) imaging experiments is used to characterize the differential electrostatic adsorption proteins synthetic polypeptides onto photopatterned monolayers at gold surfaces. The nonspecific negatively charged self-assembled (SAMs) 11-mercaptoundecanoic acid (MUA) found depend on protein pI, solution ionic strength, pH. pH dependence avidin a MUA SAM indicates that full monolayer adsorbs greater than 5.0, an "effective pK(a)" 3.6...
A novel surface enzymatic reaction scheme that amplifies the optical response of RNA microarrays to binding complementary DNA is developed for direct detection and analysis genomic DNA. The enzyme RNase H shown selectively repeatedly destroy from DNA−RNA heteroduplexes on gold surfaces; when used in conjunction with label-free technique plasmon resonance (SPR) imaging, oligonucleotides can be detected at a concentration 1 fM. This enzymatically amplified SPR imaging methodology then utilized...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPolarization modulation Fourier transform infrared reflectance measurements of thin films and monolayers at metal surfaces utilizing real-time sampling electronicsBarbara J. Barner, Michael Green, Edna I. Saez, Robert M. CornCite this: Anal. Chem. 1991, 63, 1, 55–60Publication Date (Print):January 1991Publication History Published online1 May 2002Published inissue 1 January...
Microfluidic channels fabricated from poly(dimethylsiloxane) (PDMS) are employed in surface plasmon resonance imaging experiments for the detection of DNA and RNA adsorption onto chemically modified gold surfaces. The PDMS microchannels used to (i) fabricate "1-D" single-stranded (ssDNA) line arrays that SPR oligonucleotide hybridization (ii) create "2-D" which a second set placed perpendicular 1-D array order deliver target solutions. In experiments, total sample volume is 500 μL; 2-D this...
The creation and characterization of histidine-tagged fusion protein arrays using nitrilotriacetic acid (NTA) capture probes on gold thin films for the study protein-protein protein-DNA interactions is described. Self-assembled monolayers 11-mercaptoundecylamine were reacted with heterobifunctional linker N-succinimidyl S-acetylthiopropionate (SATP) to create reactive sulfhydryl-terminated surfaces. NTA agents immobilized by reacting maleimide-NTA molecules sulfhydryl surface. SATP...
The kinetics of protein adsorption/desorption onto peptide microarrays was studied using real-time surface plasmon resonance (SPR) imaging. S binding interactions were examined an array composed five different peptides: N terminal and C immobilized wild-type (S1 S2), alternate sequence derived by phage display (LB2), NVOC-protected peptide, a FLAG control (F). Kinetic measurements the protein-S1 interaction analyzed to determine desorption rate constant (k(d)) 1.1 (+/-0.08) x 10(-2) s(-1),...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFluorescence and Fourier Transform surface-enhanced Raman scattering measurements of methylene blue adsorbed onto a sulfur-modified gold electrodeRoberta R. Naujok, Robert V. Duevel, M. CornCite this: Langmuir 1993, 9, 7, 1771–1774Publication Date (Print):July 1, 1993Publication History Published online1 May 2002Published inissue 1 July 1993https://doi.org/10.1021/la00031a026RIGHTS & PERMISSIONSArticle Views1460Altmetric-Citations146LEARN ABOUT...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAmide and ester surface attachment reactions for alkanethiol monolayers at gold surfaces as studied by polarization modulation Fourier transform infrared spectroscopyRobert V. Duevel Robert M. CornCite this: Anal. Chem. 1992, 64, 4, 337–342Publication Date (Print):February 15, 1992Publication History Published online1 May 2002Published inissue 15 February 1992https://doi.org/10.1021/ac00028a003RIGHTS & PERMISSIONSArticle...