A5034823736- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Vibrio bacteria research studies
- Antibiotic Resistance in Bacteria
- RNA Interference and Gene Delivery
- Groundwater flow and contamination studies
- Advanced biosensing and bioanalysis techniques
- Bacterial biofilms and quorum sensing
- Fecal contamination and water quality
- Environmental Justice and Health Disparities
- Pneumonia and Respiratory Infections
- Aquaculture disease management and microbiota
- Enzyme Structure and Function
- Wound Healing and Treatments
- Bacterial Identification and Susceptibility Testing
- Amino Acid Enzymes and Metabolism
- Insect and Pesticide Research
- Insect and Arachnid Ecology and Behavior
- SARS-CoV-2 detection and testing
- Antimicrobial Resistance in Staphylococcus
- Acne and Rosacea Treatments and Effects
- Plant and animal studies
- Cancer Research and Treatments
- Microbial Community Ecology and Physiology
Northern Michigan University
2017-2023
Michigan United
2017-2023
Boston Children's Hospital
2008-2019
Harvard University
2007-2019
New York University
2003-2009
Icahn School of Medicine at Mount Sinai
2002-2009
During the COVID-19 pandemic, wastewater-based surveillance has been shown to be a useful tool for monitoring spread of disease in communities and emergence new viral variants concern. As pandemic enters its fourth year clinical testing declined, wastewater offers consistent non-intrusive way monitor community health long term. This study sought understand how accurately represented actual burden between communities. Two varying size demographics Michigan were monitored SARS-CoV-2 March 2020...
Summary Previous work showed that a 42‐nucleotide sequence from an SP82 bacteriophage early RNA functions as 5′ mRNA stabilizer in Bacillus subtilis . Real‐time reverse transcriptase polymerase chain reaction (RT‐PCR) analysis of decay model with alterations at the 5′‐end was used to elucidate mechanism SP82‐mediated stability. A predicted 5′‐terminal stem–loop structure essential for stabilization. Increasing strength above minimum level did not result increased thorough context which...
Prokaryotic and eukaryotic RNA polymerases can use 2- to ∼4-nt RNAs, “nanoRNAs,” prime transcription initiation in vitro. It has been proposed that nanoRNA-mediated priming of likewise occur under physiological conditions vivo influence start site selection gene expression. However, no direct evidence such regulation presented. Here we demonstrate Escherichia coli nanoRNAs a growth phase-dependent manner, resulting alterations changes We further define sequence element determines, part,...
ABSTRACT A 254-nucleotide model mRNA, designated Δ ermC was used to study the effects of translational signals and ribosome transit on mRNA decay in Bacillus subtilis . features a strong ribosome-binding site (RBS) 62-amino-acid-encoding open reading frame, followed by transcription terminator structure. Inactivation RBS or start codon resulted fourfold decrease half-life, demonstrating importance ternary complex formation for stability. Data mRNAs with stop codons at positions increasingly...
The cupA gene cluster of Pseudomonas aeruginosa encodes components and assembly factors a putative fimbrial structure that enable this opportunistic pathogen to form biofilms on abiotic surfaces. In P. the control expression is complex, with H-NS-like MvaT protein functioning repress phase-variable (on/off) operon. Here we identify four positive regulators expression, including three unusual encoded by cgrABC genes Anr, global regulator anaerobic expression. We show are expressed in manner...
Bacterial anti-sigma factors typically regulate sigma factor function by restricting the access of their cognate to RNA polymerase (RNAP) core enzyme. The Escherichia coli Rsd protein forms a complex with primary factor, sigma(70), inhibits sigma(70)-dependent transcription in vitro, and has been proposed as sigma(70)-specific thereby facilitating utilization alternative factors. In prior work, shown interact conserved region 4 but it is not known whether this interaction suffices account...
The CrbS/R two-component signal transduction system is a conserved regulatory mechanism through which specific Gram-negative bacteria control acetate flux into primary metabolic pathways. governs expression of acetyl-CoA synthase (acsA), an enzyme that converts to acetyl-CoA, metabolite at the nexus cell's most important energy-harvesting and biosynthetic reactions. During infection, can utilize this hijack host metabolism alter course colonization pathogenesis. In toxigenic strains Vibrio...
Summary A deletion derivative of the ermC gene was constructed that expresses a 254‐nucleotide mRNA. The small size this mRNA facilitated detection processing products did not differ greatly in from full‐length transcript. In presence erythromycin, which induces ribosome stalling near 5 ′ end mRNA, cleaved endonucleolytically at site stalling. Only downstream product cleavage detectable; upstream apparently too unstable to be detected. accumulated times after rifampicin addition, suggesting...
RNase J1, a ribonuclease with 5′ exonuclease and endonuclease activities, is an important factor in Bacillus subtilis mRNA decay. A model for J1 activity turnover has binding to the end tracking target site downstream, where it makes decay-initiating cleavage. The upstream fragment from this cleavage degraded by 3′ exonucleases; downstream activity. Previously, Δ ermC was used show 5′-end dependence of turnover. Here we probe J1-dependent degradation, results were consistent aspects model....
The TetL antiporter from the Bacillus subtilis inner membrane is a tetracycline-divalent cation efflux protein that energized by electrochemical proton gradient across membrane. In this study, we expressed tetL in Escherichia coli and investigated oligomeric state of detergent solution. Evidence for an emerged SDS-PAGE Western blot analysis samples as well purified cells two differently tagged species. Furthermore, no formation or restoration oligomers occurred upon solubilization Rather,...
is an important opportunistic pathogen that employs a type III secretion system (T3SS) to inject effector proteins into host cells. Using protein depletion system, we show the endoribonuclease RNase E positively regulates expression of T3SS genes. We also present evidence antagonizes genes VI and limits biofilm production in
Rapid identification of bacteria is critical in clinical and food safety applications. This paper describes a novel instrument data analysis method for identifying based on the measurement laser light scattering as beam interacts with bacterial cells suspended water. A description technology followed by an performance study set strains from genus Staphylococcus (the inclusive target organisms) non-Staphylococcus exclusive organisms). were grown sheep blood agar (SBA), tryptic soy agar, brain...
Objective: Inhibiting the proliferation of skin bacteria, using nitric oxide (NO), is a potential strategy to prevent infections. This study evaluated efficacy new NO releasing film dressing decrease resident human bacterial flora compared with measured microbial activity underneath control sterile Tegaderm transparent dressings. Methods: A within-subjects design sample convenience counts under experimental dressings those (three) and were applied without antiseptic preparation intact on...
Using a hands-on approach, this activity introduces students to the concept of viral spread and honey bee pathogenesis by illustrating pathogen transmission throughout hive. This activity, designed for introductory biology, virology, or microbiology classes, can be used in laboratory lecture settings. Students are provided with information on hive structure. then retrieve "pollen" distribute it colony. A UV light passed across students' hands determines which was infected, indicating...
Abstract Michigan’s water-quality standards specify that E. coli concentrations at bathing beaches must not exceed 300 per 100 mL, as determined by the geometric mean of culture-based in three or more representative samples from a given beach on day. Culture-based analyses require 18–24 h to complete, so results are available for issuing notifications (advisories closings) until day following collection. This one-day delay is problematic because can change markedly one next. qPCR-based...