A5006404917- Complement system in diseases
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Immune Cell Function and Interaction
- Advanced Software Engineering Methodologies
- Renal Diseases and Glomerulopathies
- Platelet Disorders and Treatments
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Invertebrate Immune Response Mechanisms
- Blood groups and transfusion
- T-cell and B-cell Immunology
- Systemic Lupus Erythematosus Research
- Service-Oriented Architecture and Web Services
- Trypanosoma species research and implications
- Blood Coagulation and Thrombosis Mechanisms
- Galectins and Cancer Biology
- Immunotherapy and Immune Responses
- Chronic Lymphocytic Leukemia Research
- Software Engineering Research
- Lung Cancer Treatments and Mutations
- Neonatal Respiratory Health Research
- Pancreatic function and diabetes
- Software Engineering Techniques and Practices
- Real-Time Systems Scheduling
- Model-Driven Software Engineering Techniques
Aarhus University
2016-2025
Helios Klinikum Emil von Behring
2012-2024
Hochschule Bonn-Rhein-Sieg
2024
Clinica de Pneumologie Iaşi
2023
Lungenklinik Hemer
2020
University of Southern Denmark
2003-2019
University of Copenhagen
2017
Maastricht University Medical Centre
2016
Wageningen University & Research
2016
Aarhus University Hospital
1992-2015
Mannan-binding protein (MBP) is a serum lectin participating in the innate immune defense by opsonizing various microorganisms for phagocytosis. Opsonization defect due to MBP deficiency and low levels of can partially be explained dominant effect three different mutations structural part gene. Large interracial differences frequencies these variants have previously been described, but they cannot explain large interindividual variation concentration. We describe existence additional...
Adverse outcome of critical illness is often caused by systemic inflammation and sepsis. A recent study showed that mortality significantly reduced maintenance normoglycemia using intensive insulin therapy. We examined whether the beneficial effects therapy involve modulations mannose-binding lectin (MBL) C-reactive protein (CRP) levels. From a 1548 patients randomly assigned to either conventional treatment or at an care unit (ICU) we included all 451 who needed prolonged (>5 d). CRP MBL...
Neutrophils are the main proinflammatory cell type in chronically infected lungs of cystic fibrosis (CF) patients; however, they fail to effectively clear colonizing pathogens. Here, we investigated molecular composition non-mucoid and mucoid Pseudomonas aeruginosa-induced neutrophil extracellular traps (NETs) vitro compared them DNA-protein complexes present CF sputum. The protein P. NET fragments revealed that irrespective inducing stimuli, were decorated with a conserved set proteins....
The serum lectin, mannan binding protein (MBP), was isolated in a yield of 40 micrograms/liter from pooled normal human by affinity chromatography on mannan-Sepharose, followed gel-filtration and ion-exchange finally passage down an anti-IgM Sepharose column. A rabbit antiserum prepared against the purified MBP enzyme-linked immunoassay developed that used both specificity polyclonal antibody Ca+(+)-dependent carbohydrate property MBP. Assay sera 103 blood-donors showed wide range levels,...
Abstract Mannose (or mannan)-binding lectin (MBL) is an oligomeric serum that plays a role in innate immunity by activating the complement system. In human, two types of MBL-associated serine protease (MASP-1 and MASP-2) truncated protein MASP-2 (small protein; sMAP or MAp19) are complexed with MBL. To clarify proteolytic activities MASP-1 against C4, C2, C3, we isolated these MASP activated forms from human sequential affinity chromatography. On anti-MASP-1 column, passed through column...
SUMMARY Two ELISAs for estimating mannan-binding protein (MBP) were constructed and the concentration of MBP in plasma was followed patients undergoing major surgery having a malarial attack. In both cases increases observed. The relative increase kinetics varied from person to person. increased between 1·5- three-fold following surgery. some an seen at day 1 whereas others not observed until days 3–9. malaria level maintained during 30 treatment with chloroquine. independent presurgery or...
Gram-positive organisms like Staphylococcus aureus are a major cause of morbidity and mortality worldwide. Humoral response molecules together with phagocytes play role in host responses to S. aureus. The mannose-binding lectin (MBL, also known as protein) is an oligomeric serum molecule that recognizes carbohydrates decorating broad range infectious agents including Circumstantial evidence vitro vivo suggests MBL plays key first line defense. We tested this contention directly by generating...
A receptor binding to the C1q subcomponent of complement has been reported by many workers. In this paper we report for first time that binds not only C1q, but also three other structurally similar ligands, namely mannan protein (MBP), conglutinin, and lung surfactant (SP-A). All these ligands have enhance removal species bound their globular domain from blood (MBP, C1q) or (SP-A) through phagocytosis. One possible roles ligand-receptor may be initiation
One pathway of complement activation involves mannan-binding lectin and its associated serine protease 2 (MASP-2). MASP-2 deficiency was identified in an adult with a history ulcerative colitis, erythema multiforme bullosum, possible systemic lupus erythematosus. He had recurrent episodes severe pneumococcal pneumonia. Studies documented that his not functional.
Mannan-binding lectin (MBL) forms a multimolecular complex with at least two MBL-associated serine proteases, MASP-1 and MASP-2. This initiates the MBL pathway of complement activation by binding to carbohydrate structures present on bacteria, yeast, viruses. MASP-2 are composed modular structural motifs similar those C1q-associated proteases C1r C1s. Another protein 19 kDa same N-terminal sequence as 76-kDa is consistently detected part MBL/MASP complex. In this study, we primary structure...
Abstract Mannan-binding lectin (MBL) plays a pivotal role in innate immunity by activating complement after binding carbohydrate moieties on pathogenic bacteria and viruses. Structural similarities shared MBL C1 complexes the MBL- C1q-associated serine proteases, MBL-associated protease (MASP)-1 MASP-2, C1r C1s, respectively, have led to expectation that pathways of activation are likely be very similar. We expressed rMASP-2 show that, whereas complex autoactivation proceeds via two-step...
L-ficolin and H-ficolin are molecules of the innate immune system. Upon recognition a suitable target they activate complement The ligand structure ficolins is contained within fibrinogen-like domain. We examined selectivity through inhibiting binding to bacteria or beads coupled with N-acetylglucosamine. Streptococcus pneumoniae 11F was inhibited by N-acetylated sugars not non-acetylated sugars. However, it also other acetylated compounds. Based on this easily defined as lectin. Aerococcus...
Mannan-binding lectin (MBL), L-ficolin, and H-ficolin are pattern recognition molecules of the innate immune system. We investigated their ability to bind different serotypes noncapsulated variants two gram-positive bacterial species, Streptococcus pneumoniae Staphylococcus aureus. MBL did not capsulated S. aureus or but a variant (Wood). L-ficolin bound some (serotypes 1, 8, 9, 11, 12) (11A, 11D, 11F) strains. any included in this study one strain Aerococcus viridans. The concentrations...