A5003081676- Antimicrobial Resistance in Staphylococcus
- Inflammasome and immune disorders
- Advanced Nanomaterials in Catalysis
- Clostridium difficile and Clostridium perfringens research
- Endoplasmic Reticulum Stress and Disease
- Salmonella and Campylobacter epidemiology
- Listeria monocytogenes in Food Safety
- Complement system in diseases
- Trace Elements in Health
- Streptococcal Infections and Treatments
- Toxoplasma gondii Research Studies
- Antimicrobial Peptides and Activities
- S100 Proteins and Annexins
- Mosquito-borne diseases and control
- Invertebrate Immune Response Mechanisms
- Cancer Research and Treatments
- Bacterial biofilms and quorum sensing
- Amoebic Infections and Treatments
- Bacterial Identification and Susceptibility Testing
Vanderbilt University Medical Center
2021-2024
University of Pennsylvania
2017-2022
Inflammasomes are cytosolic multiprotein complexes that initiate host defense against bacterial pathogens by activating caspase-1-dependent cytokine secretion and cell death. In mice, specific nucleotide-binding domain, leucine-rich repeat-containing family, apoptosis inhibitory proteins (NAIPs) activate the CARD domain-containing protein 4 (NLRC4) inflammasome upon sensing components of type III system (T3SS) flagellar apparatus. NAIP1 recognizes T3SS needle protein, NAIP2 inner rod NAIP5...
Salmonella enterica serovar Typhimurium is a Gram-negative pathogen that uses two distinct type III secretion systems (T3SSs), termed pathogenicity island (SPI)-1 and SPI-2, to deliver virulence factors into the host cell. The SPI-1 T3SS enables invade cells, while SPI-2 facilitates Salmonella's intracellular survival. In mice, family of cytosolic immune sensors, including NAIP1, NAIP2, NAIP5/6, recognizes needle, inner rod, flagellin proteins, respectively. Ligand recognition triggers...
Staphylococcus aureus is responsible for a substantial number of invasive infections globally each year. These are problematic because they frequently recalcitrant to antibiotic treatment. Antibiotic tolerance, the ability bacteria persist despite normally lethal doses antibiotics, contributes treatment failure in S. infections. To understand how tolerance induced, biofilms exposed multiple anti-staphylococcal antibiotics examined using both quantitative proteomics and transposon sequencing....
Cellular respiration is essential for multiple bacterial pathogens and a validated antibiotic target. In addition to driving oxidative phosphorylation, has variety of ancillary functions that obscure its contribution pathogenesis. We find here the intracellular pathogen Listeria monocytogenes encodes two respiratory pathways which are partially functionally redundant indispensable Loss decreased NAD + regeneration, but this could be specifically reversed by heterologous expression...
The complement-like pathway of the African malaria mosquito Anopheles gambiae provides protection against infection by diverse pathogens. A functional requirement for a core set proteins during infections rodent and human parasites, bacteria, fungi suggests similar mechanism operates different However, extent to which molecular mechanisms are conserved is unknown. In this study we probed biochemical responses challenge Gram-positive bacterium Staphyloccocus aureus. Western blot analysis...
ABSTRACT The host protein calprotectin inhibits the growth of a variety bacterial pathogens through metal sequestration in process known as “nutritional immunity.” Staphylococcus aureus is inhibited by vitro , and localized vivo to staphylococcal abscesses during infection. However, adaptations that provide defense against nutritional immunity role metal-responsive regulators are not fully characterized. In this work, we define transcriptional response S. regulators, Zur, Fur, MntR,...
is responsible for a substantial number of invasive infections globally each year. These are problematic because they frequently recalcitrant to antibiotic treatment, particularly when caused by Methicillin-Resistant
Abstract Salmonella enterica serovar Typhimurium is a Gram-negative pathogen that uses two distinct type III secretion systems (T3SSs), termed pathogenicity island (SPI)-1 and SPI-2, to deliver virulence factors into the host cell. The SPI-1 T3SS enables invade cells, while SPI-2 facilitates ’s intracellular survival. In mice, family of cytosolic immune sensors, including NAIP1, NAIP2, NAIP5/6, recognizes needle, inner rod, flagellin proteins, respectively. Ligand recognition triggers...
Abstract Cellular respiration is essential for multiple bacterial pathogens and a validated antibiotic target. In addition to driving oxidative phosphorylation, has variety of ancillary functions that obscure its contribution pathogenesis. We find here the intracellular pathogen Listeria monocytogenes encodes two respiratory pathways which are partially functionally redundant indispensable Loss decreased NAD + regeneration, but this could be specifically reversed by heterologous expression...