A5058995672- Identification and Quantification in Food
- Environmental DNA in Biodiversity Studies
- Forensic and Genetic Research
- Genetic diversity and population structure
- Molecular Biology Techniques and Applications
- Wildlife Ecology and Conservation
- Genomics and Phylogenetic Studies
- Forensic Anthropology and Bioarchaeology Studies
- Isotope Analysis in Ecology
- Forensic Entomology and Diptera Studies
- Pleistocene-Era Hominins and Archaeology
- Forensic Fingerprint Detection Methods
- Microbial Community Ecology and Physiology
- Animal Ecology and Behavior Studies
- Archaeology and ancient environmental studies
- Archaeological Research and Protection
- Plant-Microbe Interactions and Immunity
- Metabolomics and Mass Spectrometry Studies
- Insect behavior and control techniques
- Evolution and Paleontology Studies
- Plant Taxonomy and Phylogenetics
- Plant Pathogens and Fungal Diseases
- Insect symbiosis and bacterial influences
- Conservation Techniques and Studies
- Plant Disease Resistance and Genetics
Murdoch University
2020-2025
Flinders University
2013-2019
Arcadia University
2016-2019
University of Strathclyde
2006-2015
International Union of Pure and Applied Chemistry
2006-2009
Luminol, leuchomalachite green, phenolphthalein, Hemastix, Hemident, and Bluestar are all used as presumptive tests for blood. In this study, the were subjected to dilute blood (from 1:10,000 1:10,000,000), many common household substance, chemicals. Samples tested DNA determine whether damaged or destroyed DNA. The loci D2S1338 D19S433. Leuchomalachite green had a sensitivity of 1:10,000, while remaining able detect dilution 1:100,000. Substances include saliva, semen, potato, tomato,...
The phylogeny and taxonomy of mammalian species were originally based upon shared or derived morphological characteristics. However, genetic analyses have more recently played an increasingly important role in confirming existing establishing often radically different groupings phylogenies. two most commonly used loci identification are the cytochrome oxidase I gene (COI) b (cyt b). For first time this study provides a detailed comparison effectiveness these reconstructing mammals at levels...
The extent of wildlife crime is unknown but it on the increase and has observable effects with dramatic decline in many species flora fauna. growing awareness this area criminal activity reflected research papers animal DNA testing, either for identification or genetic linkage a sample to particular organism. This review focuses use testing investigations. Species relies primarily loci within mitochondrial genome; focusing cytochrome b oxidase 1 genes. gained early prominence through its...
Nucleic acid staining dyes are used for detecting nucleic acids in electrophoresis gels. Historically, the most common dye gel is ethidium bromide, however due to its toxicity and mutagenicity other that safer user environment preferred. This Short Communication details properties of now available their sensitivity detection DNA ability permeate cell membrane. It was found GelRed™ sensitive safest use with UV light excitation, both GelGreen™ Diamond™ Acid Dye were using blue excitation.
Real-time PCR (qPCR) is widely used in the life sciences. For quantifying DNA, a standard curve required. Common methods for development are time consuming, costly, necessitate specific skill set, and pose contamination risk. Using targeted synthetic oligonucleotide, such as gBlocks® Gene Fragment, overcomes these drawbacks provides researchers an accurate quick solution to development. Here, we demonstrate that using gBlocks fragment comparable sensitivity, reliability, assay performance...
Abstract A novel species‐specific multiplex to identify 18 common European mammalian species (badger, cat, cow, dog, donkey, fox, goat, guinea pig, harvest mouse, hedgehog, horse, house human, rabbit, rat, red deer and sheep), many of which are often associated with forensic investigations, has been developed. The assay is based on the mitochondrial cytochrome b gene, commonly used in identification phylogeny studies. Areas homology variation were identified create universal primers. primers...
Abstract More than 97% of the world’s freshwater reserves are found in aquifers, making groundwater one most important resources on planet. Prokaryotic communities underpin turnover energy and matter while also maintaining purity. Thus, knowledge microbial transport subsurface is crucial for health. Here, we describe first time importance stygofauna as vectors prokaryotes. The “hitch-hiking” prokaryotes associated with may be up to 5 orders magnitude higher abundance transported 34× faster...
The composition and diversity of bacteria forming the microbiome parasitic organisms have implications for differential host pathogenicity host-parasite co-evolutionary interactions. pathogens can therefore consequences that are relevant managing disease prevalence impact on affected hosts. Here, we investigate an invasive fly Philornis downsi, recently introduced to Galápagos Islands, where it poses extinction threat Darwin's finches other land birds. Larvae infest nests consume blood...
We report on the effects of six dyes used in detection DNA process extraction, amplification, and STR loci. While can be to detect presence DNA, their use is restricted if they adversely affect subsequent typing processes. Diamond™ Nucleic Acid Dye, GelGreen™, GelRed™, RedSafe™, SYBR(®) Green I, EvaGreen™ were evaluated this study. The percentage dye removed during extraction was determined be: 70.3% for I; 99.6% RedSafe™; 99.4% EvaGreen™; 52.7% Dye; 50.6% and; could not GelGreen™. It then...
Non-invasive genetic sampling using scats has a well established role in conservation biology, but rarely been applied to reptiles. Using from captive and wild Egernia stokesii (Squamata, Scincidae) we evaluated two storage six DNA-extraction methods the reliability of subsequent genotype sequence data. Accurate data were obtained frozen dried lizard scat DNA extracted QIAamp® Stool Mini Kit modified Gentra® Puregene® method, success rates reduced for scats. Wild E. eat more plants than...