A5083830818- RNA Research and Splicing
- RNA modifications and cancer
- Colorectal Cancer Treatments and Studies
- Telomeres, Telomerase, and Senescence
- Genetic factors in colorectal cancer
- Light effects on plants
- Plant Genetic and Mutation Studies
- RNA regulation and disease
- Mitochondrial Function and Pathology
- Cancer-related molecular mechanisms research
- Autophagy in Disease and Therapy
- DNA Repair Mechanisms
- Genetic Neurodegenerative Diseases
- Cancer Cells and Metastasis
- RNA Interference and Gene Delivery
- Epigenetics and DNA Methylation
- RNA and protein synthesis mechanisms
- Cytokine Signaling Pathways and Interactions
- Axon Guidance and Neuronal Signaling
- PI3K/AKT/mTOR signaling in cancer
- Cellular transport and secretion
- Seaweed-derived Bioactive Compounds
- Cancer Mechanisms and Therapy
- Melanoma and MAPK Pathways
- Endoplasmic Reticulum Stress and Disease
University of Naples Federico II
2011-2023
University of Campania "Luigi Vanvitelli"
2022
Casa Sollievo della Sofferenza
2022
Istituto per il Sistema Produzione Animale in Ambiente Mediterraneo
2020
National Research Council
2020
Institute for Agricultural and Forest Systems in the Mediterranean
2020
Hospital Universitário Clementino Fraga Filho
2014
Istituto Nazionale di Fisica Nucleare, Sezione di Napoli
2013
Federico II University Hospital
2002-2006
Previous studies showed that the combination of an anti-Epidermal growth factor (EGFR) and a MEK-inhibitor is able to prevent onset resistance anti-EGFR monoclonal antibodies in KRAS-wild type colorectal cancer (CRC), while same reverts primary KRAS mutated CRC cell lines. However, rapid limit therapies CRC.We generated four different lines resistant cetuximab (an antibody) refametinib (a selective MEK-inhibitor) after continuous exposure increasing concentration drugs. We characterized...
Abstract Background Many small vertebrates on islands grow larger, mature later, lay smaller clutches/litters, and are less sexually dimorphic aggressive than their mainland relatives. This set of observations is referred to as the 'Island Syndrome'. The syndrome linked high population density islands. We predicted that when low and/or fluctuating insular may evolve correlated trait shifts running opposite Island Syndrome, which we collectively refer 'reversed island syndrome' (RIS)...
In this work, the effects of light quality and beneficial microbes (biofertilizer) supply on structural ecophysiological traits spinach were investigated. Plants grown under four regimens: white (WL), red-blue (RB), red-green (RG), red (R) light, with or without addition biofertilizer. RG R plants biofertilizer showed morphological typical shaded as wide leaf lamina high photosynthetic pigment content. These also exhibited a higher capacity compared to WL RB plants. The improved...
The realisation of manned space exploration requires the development Bioregenerative Life Support Systems (BLSS). In such self-sufficient closed habitats, higher plants have a fundamental role in air regeneration, water recovery, food production and waste recycling. environment, ionising radiation represents one main constraints to plant growth. this study, we explore whether low doses heavy ions, namely Ca 25 Gy, delivered at seed stage, may induce positive outcomes on growth functional...
Cellular plasticity, the ability of cells to switch from an epitheial phenotype a mesenchymal one and vice versa, plays crucial role in tumour progression metastases development. In 20-25% patients with colon cancer 18% rectal cancer, are present at time first diagnosis. They cause colorectal (CRC)-related mortality, defining stage IV CRC, which is characterized by relatively short overall survival. We previously isolated two primary adenocarcinoma cell cultures that had undergone...
Recently, extracellular RNases of the RNase A superfamily, with characteristic CKxxNTF sequence signature, have been identified in fish. This has led to recognition that these are present whole vertebrate subphylum. In fact, they comprise only enzyme family unique vertebrates. Four from zebrafish (Danio rerio) previously reported and a very low activity; some endowed, like human angiogenin, powerful angiogenic bactericidal activities. paper, we report three-dimensional structure,...
Abstract Background The "PTEN hamartoma tumor syndrome" (PHTS) includes a group of syndromes caused by germline mutations within the suppressor gene "phosphatase and tensin homolog deleted on chromosome ten" ( PTEN ), characterized multiple polyps in gastrointestinal tract highly increased risk developing malignant tumours many tissues. current work clarifies molecular basis PHTS three unrelated Italian patients, sheds light pathway disregulation constitutively associated to alteration....
chlorpyrifos (CPF) is an organophosphate insecticide used to control pests on a variety of food and feed crops. In mammals, maternal exposure CPF has been reported induce cerebral cortex thinning, alteration long-term brain cognitive function, Parkinson-like symptoms, but the mechanisms these processes are not fully understood. this study, we aimed gain deeper understanding alterations induced in brains mice chronically exposed by dietary intake. For our purpose, analysed F1 offspring...
Abstract The HEXIM1 protein, in association with 7SK snRNA, binds and inhibits the kinase activity of P‐TEFb (CDK9/cyclin T). is crucial for efficient transcription elongation viral cellular genes. was originally isolated as a protein up‐regulated by hexamethylene bisacetamide (HMBA), prototypical inducer differentiation. To determine causative role during cell differentiation we analyzed biochemical functional consequences levels several vitro systems. We found that mRNA are murine...
ABSTRACT Sexual differentiation (SD) during development results in anatomical, metabolic, and physiological differences that involve not only the gonads, but also a variety of other biological structures, such as brain, determining morphology, behavior, response breeding season. In many reptiles, whose sex is determined by egg incubation temperature, leopard gecko, Eublepharis macularius , embryos incubated at different temperatures clearly differ volume brain nuclei modulate behavior. Based...
The response of higher plants to ionising radiation depends on factors related both properties and plant features including species, cultivar, age, structural complexity the target organ. Adult dwarf tomato were irradiated with different doses X-rays investigate possible variations in leaf morpho-anatomical traits, photosynthetic efficiency, genomic DNA. In order assess if how responses depend developmental stage, we analysed two types leaves; nearly mature leaves (L1) actively developing...
In eukaryotic cells, each molecule of H/ACA small nucleolar RNA (snoRNA) assembles with four evolutionarily conserved core proteins to compose a specific ribonucleoprotein particle. One the components has pseudouridine synthase activity and catalyzes conversion selected uridine pseudouridine. Members family are highly conserved. addition catalyzing pseudouridylation target RNAs, they carry out variety essential functions related ribosome biogenesis and, in mammals, telomere maintenance. To...
Identification of alternatively spliced transcripts produced by a gene is crucial step in deciphering the bulk its biological roles and overall processes that regulate activity. By using combination bioinformatic molecular approaches we identified, cloned, characterized 3 novel alternative splice isoforms derived from human dyskeratosis congenita 1 (hDKC1), an essential causative X-linked disease involved multiple functions related to cell growth, proliferation, telomere maintenance....
Colorectal cancer (CRC) is the third most prevalent type of worldwide. It also second common cause cancer‑associated mortality; it accounted for about 9.2% all deaths in 2018, which were due to resistance therapy. The main treatment CRC surgery, generally associated with chemotherapy, radiation therapy and combination However, while chemo‑radiotherapy kills differentiated cells, mesenchymal stem‑like cells are resistant this treatment, can give rise therapy‑resistant tumors. Our previous...
Here we report the results obtained for a strain isolated from polluted site and classified as Azospira sp. OGA 24. The capability of 24 to utilize perchlorate nitrate regulation pathways were investigated by growth kinetic studies analysis messenger RNA (mRNA) expression genes reductase alpha subunit (pcrA), chlorite dismutase (cld), periplasmic large (napA). In aerobic conditions in minimal medium containing 10 mM acetate carbon source, 5.6 ± 0.34 mmol L−1 or 9.7 0.22 efficiently reduced...
The human DKC1 gene encodes dyskerin, an evolutionarily conserved nuclear protein whose overexpression represents a common trait of many types aggressive sporadic cancers. As crucial component the H/ACA snoRNP complexes, dyskerin is involved in variety essential processes, including telomere maintenance, splicing efficiency, ribosome biogenesis, snoRNAs stabilization and stress response. Although multiple minor isoforms have been identified, their functions remain to be defined. Considering...
The invasive macroalga Caulerpa cylindracea has spread widely in the Mediterranean Sea, becoming a favorite food item for native fish reasons yet unknown. By using combination of behavioral, morphological, and molecular approaches, herein we provide evidence that bisindole alkaloid caulerpin, major secondary metabolite C. cylindracea, significantly increases intake model Danio rerio, influencing regulation genes involved orexigenic pathway. In addition, found compound improves reproductive...