A5103012558- Salmonella and Campylobacter epidemiology
- Bacteriophages and microbial interactions
- Plant Molecular Biology Research
- Cancer Research and Treatments
- Plant Virus Research Studies
- Genomics and Phylogenetic Studies
- Plant Disease Resistance and Genetics
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- Plant and Fungal Interactions Research
- Vibrio bacteria research studies
- Listeria monocytogenes in Food Safety
- Influenza Virus Research Studies
- SARS-CoV-2 and COVID-19 Research
- Plant Pathogenic Bacteria Studies
- Antibiotic Resistance in Bacteria
- Viral gastroenteritis research and epidemiology
- Cocoa and Sweet Potato Agronomy
- Oil Spill Detection and Mitigation
- Pharmaceutical and Antibiotic Environmental Impacts
- Brucella: diagnosis, epidemiology, treatment
- COVID-19 Clinical Research Studies
- Identification and Quantification in Food
- Plant Gene Expression Analysis
- Microbial bioremediation and biosurfactants
Public Health Agency of Canada
2024-2025
McGill University
2019-2023
Canadian Food Inspection Agency
2019-2023
Shaanxi Research Design Institute of Petroleum and Chemical Industry
2021
National University of Singapore
2012-2020
University of Guelph
2019-2020
Université Laval
2019-2020
Agriculture and Agri-Food Canada
2016-2019
The Canadian Sentinel Practitioner Surveillance Network reports mid-season 2023/24 influenza vaccine effectiveness (VE) of 63% (95% CI: 51–72) against A(H1N1)pdm09, lower for clade 5a.2a.1 (56%; 95% 33–71) than 5a.2a (67%; 48–80), and lowest A(H3N2) (40%; 5–61). Omicron XBB.1.5 protected comparably well, with VE 47% 21–65) medically attended COVID-19, higher among people reporting a prior confirmed SARS-CoV-2 infection at 67% 28–85).
The Canadian Sentinel Practitioner Surveillance Network (SPSN) reports interim 2024/25 vaccine effectiveness (VE) against acute respiratory illness due to laboratory-confirmed influenza during a delayed season of predominant A(H1N1)pdm09 and lower A(H3N2) co-circulation. Through mid-January, the risk outpatient A is reduced by about half among vaccinated vs unvaccinated individuals. Adjusted VE 53% (95% CI: 36-65) A(H1N1)pdm09, comprised clades 5a.2a 5a.2a.1, 54% 29-70) A(H3N2), virtually...
The developmental functions of miR156-SPL regulatory network have been extensively studied in Arabidopsis, but the downstream genes regulated by each SPL not well characterized. In this study, Next Generation Sequencing-based transcriptome analysis was performed on roots wild type (WT) and miR156 overexpression (miR156OE) plants. One genes, SPL10, which represses lateral root growth significantly downregulated miR156OE A transcription factor, AGAMOUS-like MADS box protein 79 (AGL79), also...
Medicago sativa (alfalfa) is a low-input forage and potential bioenergy crop, improving its yield quality has always been focus of the alfalfa breeding industry. Transgenic plants overexpressing precursor microRNA156 (MsmiR156) were recently generated by our group. These (miR156OE) showed enhanced biomass yield, reduced internodal length, increased shoot branching trichome density, delay in flowering time. Transcripts three SQUAMOSA-PROMOTER BINDING PROTEIN-LIKE (SPL) genes (MsSPL6, MsSPL12,...
Infections with protozoan parasites associated the consumption of fresh produce is an on-going issue in developed countries but mitigating risk hampered by lack adequate methods for their detection and identification. We a metagenomic next-generation sequencing (mNGS) assay using MinION sequencer identification intentionally contaminated lettuce to achieve more accurate rapid method than traditional molecular microscopy commonly used regulatory purposes. Lettuce (25 g) was spiked varying...
Salmonella Infantis, a common contaminant of poultry products, is known to harbor mobile genetic elements that confer multi-drug resistance (MDR) and have been detected in many continents. Here, we report four MDR S. Infantis strains recovered from house environments Santa Cruz Island the Galapagos showing extended-spectrum β-lactamase (ESBL) reduced fluoroquinolone susceptibility. Whole-genome sequencing (WGS) revealed presence ESBL-conferring blaCTX-M-65 gene an IncFIB-like plasmid three...
Abstract Turnip crinkle virus (TCV) is a carmovirus that infects many Arabidopsis ecotypes. Most studies mainly focused on discovery of resistance genes against TCV infection and there no Next Generation Sequencing based comparative genome wide transcriptome analysis reported. In this study, RNA-seq revealed 238 (155 up-regulated 83 down-regulated) significant differentially expressed with at least 15-fold change were determined. Fifteen (including upregulated, unchanged downregulated)...
Viruses contain either DNA or RNA as genomes. viruses replicate within nucleus, while most viruses, especially (+)-sense single-stranded RNA, and are present cytoplasm. We proposed a new thought that is contrary to the common notion only in In this study, we question whether genome of plant virus (non-retroviral) nucleus infected cells? Hibiscus chlorotic ringspot (HCRSV) was detected cells, shown by fluorescent situ hybridization. Western blot using anti-histone 3 anti-phosphoenolpyruvate...
Abstract Background Bacteriophages are bacterial parasites and considered the most abundant diverse biological entities on planet. Previously we identified 154 prophages from 151 serovars of Salmonella enterica subsp. . A detailed analysis prophage genomics is required given influence phages their hosts should provide a broader understanding biology virulence contribute to practical applications as vectors antibacterial agents. Results Here comparative full genome sequences 142 which...
Virus infection may cause a multiplicity of symptoms in their host including discoloration, distortion and growth retardation. Hibiscus chlorotic ringspot virus (HCRSV) was studied using kenaf (Hibiscus cannabinus L.), non-wood fiber-producing crop this study. Infection by HCRSV reduced the fiber yield concomitant economic value kenaf. We investigated retardation fluctuations four selected miRNAs after infection. Vegetative (including plant height, leaf size root development) severely...
The rapid detection of foodborne microbial pathogens contaminating fresh fruits and vegetables during the intervening period between harvest consumption could revolutionize quality assurance food usually consumed raw those with a limited shelf life. We have developed sensitive, shotgun whole genome sequencing protocol capable detecting as few 1 colony forming unit (cfu) Salmonella enterica serovar Typhimurium spiked on 25 g lettuce. Ion Torrent platform was used to generate reads globally...
We have developed a targeted, amplicon-based next-generation sequencing method to detect and analyze 227 virulence genes (VG) of Salmonella (AmpliSeqSalm_227VG) for assessing the pathogenicity potential Salmonella. The procedure was using 80 reference genomes representing 75 epidemiologically-relevant serovars associated with human salmonellosis. applied AmpliSeqSalm_227VG assay (a) 35 previously characterized field strains consisting commonly incriminated in foodborne illnesses (b) 34...
The p23 is a unique protein in the Hibiscus chlorotic ringspot virus which belongs to Family Tombusviridae Genus Carmovirus. Our previous results showed that indispensable for host-specific replication and localized nucleus with novel nuclear localization signal. To investigate additional function(s) of p23, mutations basic amino acids lysine (K), arginine (R) histidine (H) abolish its localization, were introduced into biologically active full-length cDNA clone p223 HCRSV testing effects on...
Abstract The chemotactic properties of an oil-degrading Pseudomonas aeruginosa strain 6-1B, isolated from Daqing Oilfield, China, have been investigated. 6-1B could grow well in crude oil with a specific rhamnolipid biosurfactant production. Furthermore, it exhibits chemotaxis toward various substrates, including glycine, glycerol, glucose, and sucrose. Compared another strain, T7-2, the presented better response towards its vital component, n -alkenes. Based on observed distribution cells...
Polymerase chain reaction (PCR) is commonly used to detect Listeria monocytogenes, foodborne pathogen. This study conducted in silico genomic analysis investigate the specificity and binding efficacy of four published pairs PCR primers targeting prfA-virulence gene cluster (pVGC) based on sequences available. We first performed comprehensive analyses pVGC, main pathogenicity island spp. In total, 2961 prfA, 642 plcB, 629 mpl, 1181 hlyA were retrieved from NCBI database. Multiple sequence...
Complete genome sequences of eight isolates Salmonella enterica subsp. from Canadian wild birds were determined by MinION and Illumina MiSeq sequencing. Assembled chromosomes had an average size 4,833,662 bp. serovar Worthington obtained partridge quail carried 267-kb plasmids, which contained multiple antimicrobial resistance genes.
The complete genome sequences of 12 isolates the rare Salmonella enterica serovar Adjame were determined by combining Nanopore and Illumina sequence reads. Chromosome sizes ranged from 4,597,011 bp to 4,678,052 bp, GC content was 52.3%. A virulent plasmid 87,433 found in only one isolate.