In Escherichia coli, p-aminobenzoate (PABA) is synthesized from chorismate and glutamine in two steps. Aminodeoxychorismate synthase components I II, encoded by pabB pabA, respectively, convert to 4-amino-4-deoxychorismate (ADC) glutamate, respectively. ADC lyase, pabC, converts PABA pyruvate. We reported that pabC had been cloned mapped 25 min on the E. coli chromosome (J. M. Green B. P. Nichols, J. Biol. Chem. 266:12971-12975, 1991). Here we report nucleotide sequence of including a...

In Escherichia coli, chorismate lyase catalyzes the first step in ubiquinone biosynthesis, conversion of to 4-hydroxybenzoate. 4-Hydroxybenzoate is converted 3-octaprenyl-4-hydroxybenzoate by 4-hydroxybenzoate octaprenyltransferase. These two enzymes are encoded ubiC and ubiA, respectively, have been reported map near one another at 92 min on E. coli chromosome. We cloned ubiCA gene cluster determined nucleotide sequence a portion ubiA. The abuts with previously that encodes large was...

Abstract: In January 2012, glucarpidase (Voraxaze ® ) received approval from the US Food and Drug Administration for intravenous treatment of toxic plasma methotrexate concentrations due to impaired renal clearance. Methotrexate, an antifolate agent, has been used over 60 years in various cancers. High-dose particularly useful leukemias lymphomas. However, even with aggressive hydration urine alkalinization, such regimens can lead acute dysfunction, as indicated by decreases production...

To prevent folate deficiencies, many countries supplement various foodstuffs with folic acid. This compound is a synthetic oxidised that differs from naturally occurring reduced folates in its metabolism and uptake. Notably, safety reviews of acid supplementation have not considered interactions gut bacteria. Here, we use the Caenorhabditis elegans – Escherichia coli animal– microbe model to examine possible bacterial route for It has been assumed supplements are taken up directly by worm,...

p-Aminobenzoate, a component of the vitamin folate, is one seven compounds derived from aromatic precursor chorismate in Escherichia coli. Historically gene products pabA and pabB were assumed to be sufficient for de novo p-aminobenzoate biosynthesis. Recent studies, however, have shown that these proteins, as nonidentical subunits single enzyme, act on form diffusible intermediate, most likely 4-amino-4-deoxychorismate. This intermediate then converted p-aminodeoxychorismate lyase (Nichols,...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSubstrate flux through methylenetetrahydrofolate dehydrogenase: predicted effects of the concentration on its partitioning into pathways leading to nucleotide biosynthesis or methionine regenerationJacalyn M. Green, Robert E. MacKenzie, and Rowena G. MatthewsCite this: Biochemistry 1988, 27, 21, 8014–8022Publication Date (Print):October 18, 1988Publication History Published online1 May 2002Published inissue 18 October...

Many microorganisms and plants possess the ability to synthesize folic acid derivatives de novo, initially forming dihydrofolate. All that serve as recipients donors of one-carbon units are tetrahydrofolate, which is formed from dihydrofolate by an NADPH-dependent reduction catalyzed reductase (FolA). This review discusses biosynthesis monoglutamate, its tetrahydrofolate addition glutamyl residues form folylpolyglutamates. Escherichia coli Salmonella, like many can folate appear lack...

The metabolic fate of p-aminobenzoic acid (PABA) in Escherichia coli is its incorporation into the vitamin folic acid. PABA derived from aromatic branch point precursor chorismate two steps. Aminodeoxychorismate (ADC) synthase converts and glutamine to ADC glutamate composed subunits, PabA PabB. lyase removes pyruvate ADC, aromatizes ring, generates PABA. While there much interest mechanism aminations, has been little work done on reaction. We report that requires a preincubation with...

Most mammalian cells receive exogenous folate from the bloodstream in form of 5-methyltetrahydropteroylmonoglutamate (CH3-H4PteGlu1). Because this derivative is a very poor substrate for folylpolyglutamate synthetase, enzyme that adds glutamyl residues to intracellular folates, CH3-H4PteGlu1 must first be converted tetrahydropteroylmonoglutamate (H4PteGlu1), 10-formyltetrahydropteroylmonoglutamate (CHO-H4PteGlu1), or dihydrofolate (H2 folate), which are excellent substrates synthetase....

ABSTRACT An Escherichia coli strain deficient in p -aminobenzoate synthesis was mutagenized, and derivatives were selected for growth on folic acid. Supplementation shown to be due -aminobenzoyl-glutamate present as a breakdown product commercial acid preparations. Two classes of mutations characterized by the minimum concentration that could support obtained. Both genetically physically mapped about 30 min E. chromosome. A cloned wild-type gene from this region, abgT (formerly ydaH ) confer...

Escherichia coli AbgT was first identified as a structural protein enabling the growth of p-aminobenzoate auxotrophs on exogenous p-aminobenzoyl-glutamate (M. J. Hussein, M. Green, and B. P. Nichols, Bacteriol. 180:6260-6268, 1998). The abg region includes abgA, abgB, abgT, ogt; these genes may be regulated by AbgR, divergently transcribed LysR-type protein. Wild-type cells transformed with high-copy-number plasmid encoding abgT demonstrate saturable uptake (K(T)=123 microM); control...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStudies on the polyglutamate specificity of methylenetetrahydrofolate dehydrogenase from pig liverJonathan Ross, Jacalyn Green, Charles M. Baugh, Robert E. MacKenzie, and Rowena G. MatthewsCite this: Biochemistry 1984, 23, 8, 1796–1801Publication Date (Print):April 10, 1984Publication History Published online1 May 2002Published inissue 10 April 1984https://doi.org/10.1021/bi00303a033RIGHTS & PERMISSIONSArticle Views63Altmetric-Citations28LEARN...

One-carbon (1C) metabolism is a metabolic network that integrates nutritional signals with biosynthesis, redox homeostasis, and epigenetics. There are sex differences in hepatic 1C metabolism, however, it unclear whether impact the brain. The aim of this study was to investigate if modulates effects dietary folic acid deficiency, main component 1C, brain tissue using mouse model.Male female C57Bl/6J mice were placed on deficient (FD) or control diet (CD) at six weeks until months aged. After...

Twenty-seven yeast species were tested for growth on methylamine and n-butylamine as sole nitrogen sources. Five (including Saccharomyces cerevisiae) failed to grow either amine, a further five grew only n-butylamine. The remainder both amines. Amine oxidase activity was detected in extracts of all the strains which could amines, but generally absent from cells grown ammonia or nitrate. From measurements cell-free number different it is concluded that most such have at least two amine...

The abg locus of the Escherichia coli chromosome includes three genes encoding proteins (AbgA, AbgB, and AbgT) that enable uptake utilization folate breakdown product, p-aminobenzoyl-glutamate (PABA-GLU). We report on purification characterization hydrolase (PGH) holoenzyme encoded by abgA abgB. One-step was accomplished using a plasmid carrying abgAB with hexahistidine tag carboxyl terminus AbgB subsequent metal affinity chromatography (MAC). Analysis sodium dodecyl sulfate-polyacrylamide...