A5086680168- Cytomegalovirus and herpesvirus research
- Herpesvirus Infections and Treatments
- Ubiquitin and proteasome pathways
- Toxoplasma gondii Research Studies
- Cancer-related Molecular Pathways
- Viral-associated cancers and disorders
- Immunotherapy and Immune Responses
- Interdisciplinary Research and Collaboration
- Sustainability and Climate Change Governance
- Cancer, Hypoxia, and Metabolism
- Glycosylation and Glycoproteins Research
- HIV Research and Treatment
- Innovative Approaches in Technology and Social Development
UK Research and Innovation
2021
Biotechnology and Biological Sciences Research Council
2021
University of Leeds
2014
University of Bristol
2012-2013
Friedrich-Alexander-Universität Erlangen-Nürnberg
2006-2009
We previously showed that open reading frame (ORF) UL26 of human cytomegalovirus, a member the US22 multigene family betaherpesviruses, encodes novel tegument protein, which is imported into cells in course viral infection. Moreover, we demonstrated pUL26 contains strong transcriptional activation domain and capable stimulating major immediate-early (IE) enhancer-promoter. Since this suggested an important function during initiation replicative cycle, sought to ascertain relevance by...
The human cytomegalovirus (HCMV) immediate-early 2 (IE2) transactivator has previously been shown to form intranuclear, dot-like accumulations in association with subnuclear structures known as promyelocytic leukemia protein (PML) nuclear bodies or ND10. We recently observed that IE2 can even after infection of PML knockdown cells, which lack genuine To further analyze the determinants localization, a recombinant HCMV expressing fused enhanced green fluorescent was constructed. infected...
ABSTRACT The major transactivator protein IE2p86 of human cytomegalovirus (HCMV) has previously been shown to undergo posttranslational modification by the covalent attachment SUMO proteins, termed SUMOylation, which occurs at two lysine residues located amino acid positions 175 and 180. Mutation acceptor lysines resulted in abrogation SUMOylation mammalian cells a strong reduction IE2p86-mediated transactivation. In this paper, we identify an additional interaction motif (SIM) within...
Responsible research and innovation (RRI) has been the preferred idiom for interrogating social, ethical political dimensions of science, technology roughly a decade. The uptake RRI into prominent policy organisations resulted in proliferation frameworks as makers have attempted to articulate what it means them enact RRI. Here, we draw on our experience developing an framework ERA Cofund Biotechnology. We discuss three ways that treating form knowledge production allowed us engage with...
Post-translational modifications of proteins are essential for cell function. Covalent modification by SUMO (small ubiquitin-like modifier) plays a role in multiple processes, including transcriptional regulation, DNA damage repair, protein localization and trafficking. Factors affecting trafficking particularly crucial neurons because their polarization, morphological complexity functional specialization. SUMOylation has emerged as major mediator intranuclear nucleo-cytoplasmic...
The γ‑herpesviruses Epstein-Barr virus and Kaposi's sarcoma‑associated herpesvirus are successful pathogens, each infecting a large proportion of the human population. These viruses persist for life host may contribute to number malignancies, which there currently no cures. Large‑scale proteomic-based approaches provide an excellent means increasing collective understanding proteomes these complex elucidating their numerous interactions within infected cell. large‑scale studies important...
Monobodies are small recombinant proteins designed to bind with high affinity target proteins. have been generated mimic the SIM [SUMO (small ubiquitin-like modifier)-interacting motif] present in many SUMO proteins, but their properties not determined cells. In study we characterize of two SUMO1-specific monobodies (hS1MB4 and hS1MB5) HEK (human embyronic kidney)-293 HeLa cells examine ability purify substrates from cell lines rat brain. Both hS1MB4 hS1MB5 compared favourably commercially...