A5007332988- Law and Political Science
- Molecular Biology Techniques and Applications
- Ion Channels and Receptors
- European Criminal Justice and Data Protection
- Cannabis and Cannabinoid Research
- Biochemical Analysis and Sensing Techniques
- Forensic Toxicology and Drug Analysis
- European and International Law Studies
- Gastrointestinal motility and disorders
- Neurotransmitter Receptor Influence on Behavior
Max Planck Institute of Experimental Medicine
2021-2022
Mitochondria contain their own DNA (mtDNA) and a dedicated gene expression machinery. As the mitochondrial dimensions are close to diffraction limit of classical light microscopy, spatial distribution proteins in particular mRNAs remains underexplored. Here, we establish single-molecule fluorescence situ hybridization (smFISH) combined with STED MINFLUX super-resolution microscopy (nanoscopy) visualize individual mRNA molecules associated proteins. nanoscopy revealed relationships between...
Abstract Serotonergic enterochromaffin (EC) cells of the gut epithelium are secretory sensory that communicate with vagal neurons. EC exhibit many features neurons in brain, raising hypothesis synapse-like contacts may mediate fast and directed signalling. To dissect functional, structural, molecular properties underlying serotonin release from genetically identified cells, we employed a multidisciplinary vitro approach combining intestinal epithelial cell organoid cultures,...