Thirty-one bacterial isolates out of 133 isolates, were obtained from rhizosphere Egyptian clover plants, and had variant capability for starch degradation on agar medium. The isolate E109 was the most potent being 72.5 U ml−1 2.5 amylase activity hydrolysis ratio (SHR), respectively, at 50 °C. identified based phenotypic characteristics, phylogenetic positions 16S rRNA gene analysis base sequences (submitted to NCBI Gen Bank). confirmed that this belonged genus Bacillus it closely related...

Three types of Kombucha fermented tea, rice, and barley were produced in order to study their changes biological chemical parameters. About a 2-fold increase mushroom dry weight after 8 days growth was observed with tea. The optical density the extracts gave same trend. Moreover, tea recorded highest specific rate lowest doubling time. total acidity, ethanol content, protein content incremented fermentation process reach peak 6–8 as descending order: > ≥ rice. scavenging abilities DPPH...

Rhizopus species is among the most well-known lipase producers, and its enzyme suitable for use in many industrial applications. Our research focuses on production of utilizing waste besides evaluating An extracellular was partially purified from culture broth oryzae R1 isolate to apparent homogeneity using ammonium sulfate precipitation followed by desalting via dialysis. The non-specific utmost activity recorded at pH 6, 40 °C with high stability 30 min. constants Km Vmax, calculated...

A number of 26 fungal cultures were isolated from different phosphatic sources, including phosphate fertilizers and rock phosphate. The highest percentage isolates obtained tri-phosphate fertilizer samples (9 with 34.6% for each sample) then 8 30.8% monophosphate fertilizer. These tested heavy metals biosorption on solid medium supplemented metal ions at concentration 50 ppm. Eighteen out able to grow containing metals. selected also screened removal concentrations (0–150 ppm) in liquid...

Abstract Bacterial cells can form biofilm on food contact surfaces, becoming a source of contamination with profound health implications. The current study aimed to determine some Egyptian medicinal plants antibacterial and antibiofilm effects against foodborne bacterial strains in milk plants. Results indicated that four ethanolic plant extracts, Cinnamon ( Cinnamomum verum ), Chamomile Matricaria chamomilla Marigold Calendula officinalis Sage Salvia had (12.0–26.5 mm inhibition zone...

Two bacterial strains were isolated from compost sample Egypt and identified based on morphological molecular characteristics using 16S rRNA sequence as Stenotrophomonas daejeonensis B14 Pseudomonas geniculate Y8. Optimization of fermentation factors was performed by one-factor-at-a-time (OFAT) minimum runs resolution-IV statistical (Min Run Res IV) approaches. OFAT technique achieved the maximum polysaccharide dry weight at 30.1 25.3 g/L S. P. Y8, respectively medium containing 25% (w/v)...

Abstract Heavy metals, including lead, cause serious damage to human health and the surrounding environment. Natural biosorbents arise as environmentally friendly alternatives. In this study, two of 41 isolates (8EF 17OS) were most efficient bacteria for growing on media supplemented with Pb 2+ (1000 mg/L). At high concentrations up 2000 mg/L, pioneer isolate 17OS exhibited remarkable resistance multiheavy metals. This was identified Paenibacillus dendritiformis deposited in GenBank under...

The present study aimed at developing a strategy to improve the volumetric production of PHAs by Pseudomonas fluorescens S48 using waste frying oil (WFO) as sole carbon source. For this purpose, several cultivations were set up steadily nutrients supply attain high cell density and biopolymer productivity. was examined in 14 L bioreactor one-stage batch, two-stage high-cell-density fed-batch cultures. highest value polymer content obtained after 60 h (33.7%). Whereas, batch culture increased...

Spherical homogeneous 32 nm, protein coated Pseudomonas silesiensis strain A3 CuNPs was investigated for their cytotoxicity effect as well antimicrobial and antitumor activity. estimated using human normal lung cell lines (Wi38) against with concentrations ranging from 25 to 150 μg/mL neutral red uptake assay. The study revealed that the bacterial had an impact on Wi38 viability at of 25, 50, 100 were 95.8, 91.1, 89.2 82.3%, respectively, a strong correlation coefficient (r = 0.94) IC50...

Abstract Bioconversion using fungi, as natural factory of many applicable bioactive compounds, enzymes utilizing agro-residue substrates a solid, abundant, low-cost growth and enzyme production media. This study characterized applied tannase (308 U/mg) from Aspergillus niger A8 + Trichoderma viride co-cultures pomegranate peels. The partially purified showed maximal relative activity at 37–65 °C for 10 min kinetics thermal inactivation energy high point 60 0.040/min. half-life was 37 58.6...

The minimum inhibitory concentration (MIC) and bactericidal (MBC) were measured at concentrations of 0.01–2.56 mg/mL grape mulberry leaves ethanolic extracts. MIC values ranged from 0.08 to 0.16 against Ps. aeruginosa Ps9, 0.32 each S. aureus St3, E. coli Ec3, typhi Sa1. Whereas, the MBC 1.28 tested effects extracts also studied representing effect extract with a ratio 2 all investigated isolates, except had towards St3 Ec3 2, bacteriostatic Ps9 Sa1 ≥4. bacteria found have strong ability...

Chitosan is found in the cell structure of Zygomycetes fungi. It a deacetylated chitin derivative, nature's second most abundant polysaccharide after cellulose. The current proposal used antimicrobial fungal chitosan polymer substances against foodborne bacterial pathogens. Ten isolates were isolated from soil present investigation, and was extracted wall to produce chitosan. These belonged Rhizopus, Aspergillus, Trichoderma, Penicillium genera. Rhizopus sp. code RL effective isolate...

T HE CURRENT research deals with optimization of the factors affecting lipase production under submerged culture system. The most efficient isolate R1 was identified depending on cultural and morphological characteristics together 18S rRNA sequence as Rhizopus oryzae. Using one variable at a time, maximum activity (171.8 U/mL) recorded in presence 1% fish-frying oil, mixture peptone yeast extract pH 5 8% v/v fungal inoculum after 4 days 30°C. screening significant using Plackett-Burman...

Biosynthesis of green nanomaterials using microorganisms is considered clean, eco-friendly and viable, instead the physical or chemical methods. This study aimed in biosynthesis copper nanoparticles (CuNPs) exploiting Egyptian local bacterial isolates. Sixteen copper-resistant isolates out 160 isolates; were captured from various plant rhizospheres including; chamomile, hibiscus, neem, iris, pea, beside samples collected Sharm El-Sheikh seawater. Among 16 isolates, one promising isolate I108...

In this study, a variety of probiotic strains, including Lactiplantibacillus plantarum, Lacticaseibacillus casei, Lactobacillus acidophilus, Streptococcus thermophilus, Bifidobacterium longum, Limosilactobacillus reuteri, delbrueckii subsp. bulgaricus, rhamnosus, and bifidum, were utilized for soymilk fermentation both as free cells synbiotics on agro-industrial residuals such okara, whey protein, banana peels, apple pomace, sugarcane bagasse, orange lemon peels. Among these, rhamnosus...

Fermented soymilk (soymilk yogurt) was made by fermenting with five probiotic bacterial strains (Lactobacillus plantarum ATCC 14917, Lactobacillus casei DSM 20011, acidophilus 20552, Lactococcus thermophilus 20259, and Bifidobacterium longum B41409) that were used as monocultures combined them consortia cultures. Seven pathogenic strains, E. coli O157H7, S. aureus As4, typhimurium As3, shigae As2, L. monocytogenes As1, P. aeruginosa 27853, B. cereus Dsmz 345, to study the antibacterial...

Fifty-eight fungal isolates were isolated from salt soil, whey and fish on medium supplemented with 0.5% NaCl. Out of 58 isolates, 49 capable to grow 1 % These halo-tolerant tested produce plant growth regulators (PGR) solid or in broth medium. On medium, 9 out gave indole acetic acid (IAA) which appeared red zone around index ranged 0.25 0.56. While tried IAA gibberellic (GA) presence NaCl at 0.5 1%. The maximum GA production obtained by FS12 isolate (4.32 4.52 mg/100ml) FW2 (2.71 2.92 1%...

Tannase is an inducible enzyme which hydrolysate tannin to gallic acid and used in many industries such as food pharmaceutical. This has scooped more attention recent times. A maximum production of tannase (ranged from 0.30 0.93 index from1.05 1.87 U/mg specific activity (STA)) (from 0.07 0.76 mg/ml concentration (GAC)) was achieved by 8 fungal strains out 24 yeast belonged genera; Aspergillus, Rhizopus, Trichoderma, Fusarium, Penicillium, Candida Saccharomyces. The selected fungi were grown...

Aims: Production of amino acids from black strap sugar cane molasses by Bacillus sp.R22EG1 strain and sp.R20EG2 using batch fed-batch (pulsed continuous feeding) cultures was investigated to achieve the maximum concentration free acids.

INETY TWO local bacterial isolates, from the rhizosphere and ...... soil around root system of bean (Vicia faba) grown in Kalubia Governorate Egypt, were bio-prospected for polyhydroxyalkanoate (PHA) accumulation.Three isolates accumulated ≥ 20 % PHAs, they identified as Pseudomonas fluorescens S48, Bacillus megaterium 7A B. UBF19.The tested gave maximum PHAs content on basal medium containing glucose ammonium sulfate at C/N ratio 30/1 after 72 hr 30 o C using shake flask culture...