A5056021970- Mitochondrial Function and Pathology
- Adipose Tissue and Metabolism
- Blood Coagulation and Thrombosis Mechanisms
- Renin-Angiotensin System Studies
- Sirtuins and Resveratrol in Medicine
- Alzheimer's disease research and treatments
- Vitamin C and Antioxidants Research
- Apelin-related biomedical research
- Cardiovascular Function and Risk Factors
- Neurological Disorders and Treatments
- GDF15 and Related Biomarkers
- Metabolism, Diabetes, and Cancer
- Macrophage Migration Inhibitory Factor
- Endoplasmic Reticulum Stress and Disease
University of South Florida
2021-2022
Abstract Sirtuin1 (SIRT1) and Sirtuin3 (SIRT3) protects cardiac function against ischemia/reperfusion (I/R) injury. Mitochondria are critical in response to myocardial I/R injury as disturbance of mitochondrial dynamics contributes dysfunction. It is hypothesized that SIRT1 SIRT3 components maintaining mitochondria homeostasis especially exert cardioprotective actions under stress. The results demonstrated deficiency aged (24–26 months) mice hearts led the exacerbated dysfunction terms...
Ischemic heart disease (IHD) is the leading cause of death, with age range being primary factor for development. The mechanisms by which aging increases vulnerability to ischemic insult are not well understood. We aim use single-cell RNA sequencing discover transcriptional differences in various cell types between aged and young mice, may contribute aged-related insult. Utilizing 10× Genomics Single-Cell sequencing, we were able complete bioinformatic analysis identity novel differential...
APC (activated protein C) is a plasma serine protease with anticoagulant and anti-inflammatory activities. EPCR (Endothelial C receptor) associated APC's activity mediates its downstream signaling events. exerts cardioprotective effects during ischemia reperfusion (I/R). This study aims to characterize the role of APC-EPCR axis in ischemic insults aging.Young (3-4 months) aged (24-26 wild-type C57BL/6J mice, as well point mutation (EPCRR84A/R84A) knockin mice incapable interaction wild type...
A progressive defect in the energy generation pathway is implicated multiple aging-related diseases, including cardiovascular conditions and Alzheimer's Disease (AD). However, evidence of pathogenesis cardiac dysfunction AD associations between two organ diseases need further elucidation. This study aims to characterize cellular defects resulting decreased function AD-model. 5XFAD mice, a strain expressing five mutations human APP PS1 that shows robust Aβ production with visible plaques at 2...
Introduction: Metformin activates AMP-activated protein kinase (AMPK) to improve cardiac function during ischemia and reperfusion (I/R). We reported that Sestrin2 (Sesn2) is associated with AMPK maintains oxidative phosphorylation (OXPHOS) under I/R stress. The role of age-related Sesn2-AMPK signaling in the beneficial actions metformin on ischemic insults remains unknown. Hypothesis: mitochondrial integrity limits damage caused by through pathway. Methods: Young (3-6 months) aged (22-24...
Introduction: Sepsis is a progressive life-threatening condition in which mortality has been consistently linked to increasing organ dysfunction. We have found that activated protein C (APC) cytoprotective and anti-inflammatory properties cardiovascular disease. Whether APC critical for maintaining systemic homeostasis under sepsis aging remains unknown. Hypothesis: Administration of recombinant ameliorates sepsis-associated renal injury cardiac dysfunction via EPCR (endothelial cell...
Introduction: The expression and secretion of cardiac MIF (macrophage migration inhibitory factor) are downregulated in aging. A small molecule agonist, MIF20 can increase the binding affinity with CD74 receptor to modulate AMPK signaling. However, pharmacological mechanisms by which triggers MIF-AMPK signaling cascade benefit heart response ischemia reperfusion (I/R) stress remain unknown. Hypothesis: rescues an impaired aging through augmenting interaction between maintain mitochondrial...
Introduction: Obesity increases the chances of developing cardiovascular disease and strokes. The overabundance adipose tissue that occurs in obese individuals oxidative stress, which trigger upregulation antioxidant proteins, Sesn2 Nrf2. Hypothesis: Obesity-related stress triggers cardiac Nrf2 to function cardioprotective properties. Methods: Cardiomyocyte specific knock out (cSesn2 -/- ) overexpression (tTa-tet-Sesn2) C57BL/6J mice, their wildtype littermates (Sesn2 f/f tet-Sesn2) were...
Introduction: Sirtuin 1 (SIRT1) is a nicotinamide adenine dinucleotide-dependent histone deacetylase. We revealed that the pressure-overload-induced SIRT1 can exacerbate cardiac systolic dysfunction. But mechanisms by which undermines function under pathological conditions remain unclear. Hypothesis: induced hypotrophy disturbs mitochondrial dynamics homeostasis resulting in an exacerbated Methods: Wild-type littermates f/f (3-5 months) and inducible cardiomyocyte-specific knockout (icSIRT1...
Introduction: Activated protein C (APC) is a vitamin-K-dependent plasma serine protease that functions as natural anticoagulant to downregulate thrombin generation in the clotting cascade. Endothelial receptor (EPCR) mediates APC's cytoprotective properties. We found cardiac EPCR expression downregulated aging, leading intolerance ischemic insults. However, role of APC human heart under stress remains unclear. Hypothesis: APC-EPCR axis protects cardiomyocytes against hypoxia-reoxygenation...
Introduction: Activated protein C (APC) is a blood serine protease that has been shown to have neuroprotective function via its downstream signaling cascade. However, there no study investigated how the lack of APC associated with Alzheimer’s disease (AD)-related neuronal changes. Hypothesis: Diminished related AD-associated biomarkers and behavioral symptoms. ameliorates these symptoms activation kinase B (Akt) pathway which inhibits glycogen synthase 3β (GSK3β) tau hyperphosphorylation....