In the study of proteins that may participate in events responsible for organization macromolecules postsynaptic membrane, we have used a mAb to an Mr 58,000 protein (58K protein) found purified acetylcholine receptor (AChR)-enriched membranes from Torpedo electrocytes. Immunogold labeling with shows 58K is located on cytoplasmic side and most concentrated near crests postjunctional folds, i.e., at sites high AChR concentration. The also recognizes skeletal muscle biochemical characteristics...

Reactive high power impulse magnetron sputtering (rHiPIMS) was used to deposit silicon nitride (SiNx) coatings for biomedical applications. The SiNx growth and plasma characterization were conducted in an industrial coater, using Si targets N2 as reactive gas. effects of different N2-to-Ar flow ratios between 0 0.3, pulse frequencies, target settings, substrate temperatures on the discharge N content investigated. Plasma ion mass spectrometry shows amounts ionized isotopes during initial...

High‐pressure freezing (HPF) permits adequate cryoimmobilization (without detectable ice crystals after freeze‐substitution) of biological tissue up to a thickness about 200 μm. Until now the preparation prior has been unsatisfactory: sizing required dimensions takes minutes, during which structural alterations must occur. We demonstrate that use fine‐needle biopsy technique minimizes damage and guarantees sample close optimal for HPF. The cores can be cryoimmobilized within 40 s excision.

The residual coating stress and its control is of key importance for the performance reliability silicon nitride (SiNx) coatings biomedical applications. This study explores most important deposition process parameters to tailor hence improve adhesion SiNx deposited by reactive high power impulse magnetron sputtering (rHiPIMS). Reactive sputter plasma characterization were conducted in an industrial chamber equipped with pure Si targets N2/Ar ambient. HiPIMS processes using N2-to-Ar flow...

This report is about occupational contact dermatitis found in 3 out of 6 workers a chemistry laboratory using Lowicryl® embedding media, which contain (meth)acrylate monomer mixtures known composition. The notation (meth) acrylates used to refer both and methacrylates. (Meth) acrylate monomers will polymerize the absence oxygen when induced by metal ions, peroxides, heat or ultraviolet light. are low viscosity remain liquid stale at temperatures far below 0°C. volatile compounds, some...

Talin is involved in mediating the cytoskeleton-extracellular matrix interaction at focal contacts cultured fibroblasts. Recently this protein has been localized both myotendinous junction (MTJ) and neuromuscular (NMJ) skeletal muscle. At MTJ, talin may mediate insertion of myofibrils into plasma membrane, thus serving a function similar to that seen contacts. However, NMJ unknown. In study, we examined its distribution mature developing acetylcholine receptor (AChR) clusters Xenopus muscle...

TiN/SiN x nanocomposite layers with Si contents between 0 and 25 at. % were deposited by a reactive arc-magnetron sputtering hybrid process. The stoichiometry of the SiNx phase was found to be related silicon sputter target state, i.e., elemental or nitrided. TiN/SiNx Si:N ratio close 0.75 (silicon nitride) show hardness maximum at overall 5 7 %. is absent for nitrogen deficient stoichiometries SiNx. oxidation resistance composite three five times better than that pure TiN. In contrast...

Ceramic coatings deposited on orthopedic implants are an alternative to achieve and maintain high wear resistance of the metallic device, simultaneously allow for a reduction in metal ion release. Silicon nitride based (SiNx) by power impulse magnetron sputtering (HiPIMS) have shown potential use joint replacements, as result improved chemical stability combination with good adhesion. This study investigated effect N, C, Cr, Nb content tribocorrosive performance 3.7 8.8 µm thick SiNx HiPIMS...

Abstract The interface between diamond‐like amorphous hydrogenated carbon (a‐C : H) and Al 2 O 3 was analysed by sputter depth profile analysis via x‐ray photoelectron spectroscopy. X‐ray spectra were also recorded during direct ion beam deposition (CH 4 , E = 400 eV) monitoring the initial build‐up of interface. No stoichiometric compound detected, although is reduced to 3‐ x ( ∼ 0.5–1) enabling an interaction with atoms. This results in excellent adhesion a‐C H chemically inert .

In continuation of our preceding studies, we disclose long‐term experiments test more extensively the protective power novel 4H Glove. 2 compounds encountered in modern electron microscopy technique 1‐hexadecene (1‐HD) and 2‐hydroxyethyl acrylate (2‐HEA). which elicit allergic and/or toxic reactions laboratory workers, were tested for their penetration through Glove material. I‐HD has not to knowledge been on any glove 2‐HEA was known have a breakthrough Brae exceeding 240 min; contrast, it...

Abstract Purified pig pancreatic zymogen granules were subjected to free flow electrophoresis (FFE) in an acetate buffer system (acetic acid/NaOH, pH 5.5) detect the presence or absence of more than one population granules. Pig purified by differential and density gradient centrifugation FFE. Fractions analyzed for protein, α‐amylase (EC 3.2.1.1) 5′‐nucleotidase 3.1.3.5) as marker enzymes granule content membranes, respectively. Only distinct peak, with coincident activity, most protein was...

The exocrine pancreas was long thought to be composed of identical subunits, the acinar cells that store inactive forms digestive enzymes in zymogen granules (ZGs). These were generally seen as a homogeneous population vesicles. This homogeneity recently questioned: Digestive demands are answered by release specific and immunocytochemical labeling showed distinctive nonidentical populations ZGs. We have aimed at finding concomitant differences element contents. analyzed energy-dispersive...