- Plant Pathogens and Fungal Diseases
- Yeasts and Rust Fungi Studies
- Plant pathogens and resistance mechanisms
- Banana Cultivation and Research
- Plant-Microbe Interactions and Immunity
- Plant and animal studies
- Genetic diversity and population structure
- Phytoplasmas and Hemiptera pathogens
- Plant Diversity and Evolution
- Parasitic Infections and Diagnostics
- Horticultural and Viticultural Research
- Mycorrhizal Fungi and Plant Interactions
- Cocoa and Sweet Potato Agronomy
- Amoebic Infections and Treatments
- Fungal Plant Pathogen Control
- Ethnobotanical and Medicinal Plants Studies
- Plant Pathogens and Resistance
- Urban Agriculture and Sustainability
- Phytochemical compounds biological activities
- Psidium guajava Extracts and Applications
- Viral gastroenteritis research and epidemiology
- Plant Pathogenic Bacteria Studies
- Cassava research and cyanide
- Genomics and Phylogenetic Studies
- Growth and nutrition in plants
Universidade de São Paulo
2014-2025
Universidade Metodista de São Paulo
2021
Universidad de Salamanca
2020
Universidade Estadual de Campinas (UNICAMP)
2013-2018
Universidade Federal Rural do Semi-Árido
2014
Fundo de Defesa da Citricultura
2014
Fungal diseases are among the main factors limiting high yields of soybean crop. Colletotrichum isolates from plants with anthracnose symptoms were studied different regions and time periods in Brazil using molecular, morphological pathogenic analyses.Bayesian phylogenetic inference GAPDH, HIS3 ITS-5.8S rDNA sequences, morphologies colony conidia, inoculation tests on seeds seedlings performed. All clustered only truncatum species three well-separated clusters. Intraspecific genetic...
Detecting pathogens in asymptomatic plants allows for a rapid response to emerging threats, such as the introduction of new or emergence resistant strains. Frosty pod rot ( Moniliophthora roreri) significantly jeopardizes cacao production by impairing quality and yield. However, early diagnosis is challenging due its prolonged incubation period, compounded symptoms resembling those M. perniciosa, causative agent witches’ broom disease. To address this, specific sensitive quantitative...
Anthracnose is an important disease in vineyards south and southeast Brazil, the main grape‐producing regions country. This study aimed to identify causal agents of grapevine anthracnose Brazil through multilocus phylogenetic analyses, morphological characterization pathogenicity tests. Thirty‐nine Elsinoë ampelina 13 Colletotrichum spp. isolates were obtained from leaves, stems berries with symptoms collected 38 southern southeastern Brazil. For E. isolates, internal transcribed spacer (...
Invasions by fungal plant pathogens pose a significant threat to the health of agricultural ecosystems. Despite limited standing genetic variation, many invasive species can adapt and spread rapidly, resulting in losses crop yields. Here, we report on population genomics Colletotrichum truncatum, polyphagous pathogen that infect more than 460 species, an soybean Brazil. We study whole-genome sequences 18 isolates representing 10 fields from two major regions production. show Brazilian C....
Knowledge of the population structure, genetic diversity, and reproductive mode plant pathogens can help to implement effective disease management strategies. Anthracnose is one most prominent diseases in soybean mainly associated with species Colletotrichum truncatum. However, structure C. truncatum populations remains unknown. We collected isolates from 10 sites representing two Brazilian states (Mato Grosso Goiás) used 13 highly polymorphic microsatellite markers investigate pathogen....
HomePlant DiseaseVol. 104, No. 6First Report of Colletotrichum musicola Causing Soybean Anthracnose in Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseFirst BrazilT. R. Boufleur, L. Castro, F. Rogério, M. Ciampi-Guillardi, Baroncelli, and N. S. Massola JúniorT. Boufleurhttp://orcid.org/0000-0002-6357-0823Luiz de Queiroz College Agriculture (ESALQ), University São Paulo (USP), Department Plant Pathology Nematology, Piracicaba, Paulo, 13418-900, Brazil,...
Precise diagnosis of plant diseases is one the most effective tools to minimize yield losses. Colletotrichum truncatum, Corynespora cassiicola, and Sclerotinia sclerotiorum are common soilborne pathogens that affect soybeans all over world. We developed a multiplex quantitative real-time polymerase chain reaction (qPCR) assay simultaneously detect quantify three in soybean seeds survey their occurrence main production areas Brazil. Species-specific primers probes for C. truncatum cassiicola...
Colletotrichum is a large genus of plant pathogenic fungi comprising more than 200 species. In this work, we present the genome sequences four species to soybean: C. truncatum, plurivorum, musicola, and sojae. While truncatum globally considered most important pathogen, other three have been described associated with soybean only recently. The will provide insights into factors that contribute pathogenicity toward be useful for further research evolution Colletotrichum.
Abstract Distance and discrete geographic barriers play a role in isolating populations, as seed pollen dispersal become limited. Nearby populations without any barrier between them may also suffer from ecological isolation driven by habitat heterogeneity, which promote divergence local adaptation drift. Likewise, elevation gradients influence the genetic structure diversity of particularly those marginally distributed. B athysa australis (Rubiaceae) is widespread tree along gradient Serra...
Citrus crops in São Paulo State, Brazil, have been severely affected by postbloom fruit drop disease (PFD), which is caused Colletotrichum acutatum. This leads to the of up 100% young fruits. Previous studies assumed that this pathogen exhibits a clonal reproductive mode, although no population genetic conducted so far. Thus, structure six C. acutatum populations from sweet orange orchards showing PFD symptoms was determined using nine microsatellite markers, enabling inference on...
Abstract Giardia duodenalis is a flagellated intestinal protozoan responsible for infections in various hosts including humans and several wild domestic animals. Few studies have correlated environmental contamination clinical the same region. The aim of this study was to compare groups from sources through population genetic analyses verify haplotype sharing degree similarity among populations metropolitan region Campinas. results showed high diversity haplotypes substantial between G. . We...
Background Giardia duodenalis (synonyms G. lamblia and intestinalis) is an enteric protozoan parasite of a wide range mammalian hosts, including humans various domestic wild animals. There considerable genetic variability in isolates this have been divided into eight assemblages. Microsatellites markers can be used to discriminate with high level sensitivity. This study was conducted identify characterize genomic microsatellites (simple sequence repeats—SSRs), sequences one- six-nucleotide...
Downy mildew, caused by Plasmopara viticola, is the main disease affecting vineyards in subtropical Brazil. Here, we collected 94 P. viticola isolates from four organic and conventional two grape-growing states of Brazil to evaluate sensitivity quinone outside inhibitor (QoI) azoxystrobin pheno- genotyping assays. The impact location, production system QoI fungicides on population genetics structure was determined using 10 microsatellite markers. Cytochrome b sequencing revealed that 28 100%...
Rhizophora mangle L. (Rhizophoraceae) grows on aerial roots, which emerge above the water level, giving stands of this tree characteristic "mangrove" appearance. To produce in situ and ex information for genetic conservation programs species, we investigated diversity mating system one R. population. We sampled 30 adult trees a total 349 seeds Northern Brazil. genotyped all with four microsatellite loci. The average fixation index was -0.222 0.030 seeds. multilocus outcrossing rate (t m...
Abstract Seed‐borne pathogenic fungi can cause serious damage to soybean crops by reducing the germination, vigour and emergence of seeds. Special attention should be paid pathogen detection in seeds prevent its introduction disease‐free areas. Considering importance rapid successful diagnosis seed‐borne soybeans, this study evaluated a method detect Sclerotinia sclerotiorum Phomopsis spp. using quantitative polymerase chain reaction (qPCR). Naturally infested samples were subjected qPCR...
Microsatellite markers for Ocotea spp in the past timber and oil extraction are currently listed as threatened due to overexploitation.To investigate genetic diversity population structure of these species, we developed 8 polymorphic microsatellite O. odorifera from an enriched library by using 2 dinucleotide repeats.The were tested cross-amplification catharinensis porosa.The average number alleles per locus was 10.2, considering all loci over populations odorifera.Observed expected...
Anthracnose has become one of the main threats to soybean production and is considered most important disease in some soybean-producing areas. Colletotrichum truncatum species commonly associated with anthracnose produces microsclerotia. However, role microsclerotia epidemiology not yet been described. The aim this study was determine whether C. can survive maintain pathogenicity for a period up 246 days, corresponding off-season cultivation Brazil. Therefore, two pathogenic isolates...