A5100419634- Insect and Pesticide Research
- Insect and Arachnid Ecology and Behavior
- Plant and animal studies
- Insect-Plant Interactions and Control
- Plant Virus Research Studies
- Insect Pheromone Research and Control
- Insect Pest Control Strategies
- Bioactive Natural Diterpenoids Research
- Plant-based Medicinal Research
- Insect symbiosis and bacterial influences
- Bee Products Chemical Analysis
- Healthcare and Venom Research
- Plant and Fungal Interactions Research
- Berberine and alkaloids research
- Date Palm Research Studies
- HIV/AIDS drug development and treatment
- HIV Research and Treatment
- Immunotherapy and Immune Responses
- Psoriasis: Treatment and Pathogenesis
- Postharvest Quality and Shelf Life Management
- Culinary Culture and Tourism
- IoT and Edge/Fog Computing
- Wireless Body Area Networks
- Age of Information Optimization
- Potato Plant Research
Agricultural Research Service
2015-2024
United States Department of Agriculture
2012-2024
Southern University of Science and Technology
2024
Shenzhen Third People’s Hospital
2024
Xi’an University of Posts and Telecommunications
2024
Beltsville Agricultural Research Center
2008-2023
Yan'an University
2020-2021
Yanan University Affiliated Hospital
2020-2021
Xian Yang Central Hospital
2020
Guangzhou University of Chinese Medicine
2020
Over the last two winters, there have been large-scale, unexplained losses of managed honey bee (Apis mellifera L.) colonies in United States. In absence a known cause, this syndrome was named Colony Collapse Disorder (CCD) because main trait rapid loss adult worker bees. We initiated descriptive epizootiological study order to better characterize CCD and compare risk factor exposure between populations afflicted by not CCD.Of 61 quantified variables (including physiology, pathogen loads,...
Abstract Social insects are able to mount both group‐level and individual defences against pathogens. Here we focus on defences, by presenting a genome‐wide analysis of immunity in social insect, the honey bee Apis mellifera . We present models for each four signalling pathways associated with immunity, identifying plausible orthologues nearly all predicted pathway members. When compared sequenced Drosophila Anopheles genomes, bees possess roughly one‐third as many genes 17 gene families...
Recent losses in honey bee colonies are unusual their severity, geographical distribution, and, some cases, failure to present recognized characteristics of known disease. Domesticated bees face numerous pests and pathogens, tempting hypotheses that colony collapses arise from exposure new or resurgent pathogens. Here we explore the incidence abundance currently pathogens suffering Colony Collapse Disorder (CCD), otherwise weak colonies, strong across United States. Although pathogen...
SummarySummaryMethods are described for working with Nosema apis and ceranae in the field laboratory. For fieldwork, different sampling methods to determine colony level infections at a given point time, but also following temporal infection dynamics. Suggestions made how standardise trials evaluating treatments disease impact. The laboratory include means determining individual bee levels species determination, including light microscopy, electron molecular (PCR). cage trials, inoculation...
SummaryHoney bee virus research is an enormously broad area, ranging from subcellular molecular biology through physiology and behaviour, to individual colony-level symptoms, transmission epidemiology. The methods used in virology are therefore equally diverse. This article covers those that very particular virological bees, with numerous cross-referrals other BEEBOOK papers on more general methods, as well research. At the root of these realization viruses at their most primary level...
Colony collapse disorder (CCD), a syndrome whose defining trait is the rapid loss of adult worker honey bees, Apis mellifera L., thought to be responsible for minority large overwintering losses experienced by U.S. beekeepers since winter 2006-2007. Using same data set developed perform monofactorial analysis (PloS ONE 4: e6481, 2009), we conducted classification and regression tree (CART) in an attempt better understand relative importance interrelations among different risk variables...
RNA viruses impact honey bee health and contribute to elevated colony loss rates worldwide. Deformed wing virus (DWV) the closely related Varroa destructor virus-1 (VDV1), are most widespread viruses. VDV1 is known cause high of overwintering losses in Europe, however it was unknown United States (US). Using next generation sequencing, we identified pupae US. We tested 603 apiaries US 2016 found that present 66.0% them, making second prevalent after DWV, which 89.4% colonies. had highest...
The effects of sublethal pesticide exposure on queen emergence and virus titers were examined. Queen rearing colonies fed pollen with chlorpyrifos (CPF) alone (pollen-1) CPF the fungicide Pristine(®) (pollen-2). Fewer queens emerged when larvae from open foraging (i.e., outside) reared in pollen-1 or 2 compared those outside colonies. Larvae grafted pollen-2 had lower rates than Deformed wing (DWV) black cell found nurse bees viruses also detected larvae. However, we did not detect In...
Malnutrition is a major cause of colony losses. In managed hives, bees are fed protein supplements (PS) during pollen shortages. If were provided with natural forage instead PS, would they have lower pathogen levels and higher queen survival? We addressed this question by either providing colonies (Brassica rapa—rapini) or feeding them PS from November to February. Soluble concentrations in the than rapini as most amino acids. Nurse digested less pollen. Hemolymph titers nurse growth did not...
Abstract Honey bees, the primary managed insect pollinator, suffer considerable losses due to Deformed wing virus (DWV), an RNA vectored by mite Varroa destructor . Mite vectoring has resulted in emergence of virulent DWV variants. The basis for such changes is poorly understood. Most importantly, it remains unclear whether replication occurs mite. In this study, we exposed mites type A via feeding on artificially infected honey bees. significant, 357-fold increase load was observed these...
The impacts of invertebrate RNA virus population dynamics on virulence and infection outcomes are poorly understood. Deformed wing (DWV), the main viral pathogen honey bees, negatively bee health, which can lead to colony death. Despite previous reports reduction DWV diversity following arrival parasitic mite Varroa destructor, key vector, we found high genetic in infested United States colonies. Phylogenetic analysis showed that divergent US genotypes monophyletic origin were likely...
Sublethal exposure to fungicides can affect honey bees (Apis mellifera L.) in ways that resemble malnutrition. These include reduced brood rearing, queen loss, and increased pathogen levels. We examined the effects of oral boscalid pyraclostrobin on factors affecting colony nutrition immune function including pollen consumption, protein digestion, hemolymph titers, changes virus Because are respiratory inhibitors, we also measured ATP concentrations flight muscle. The were evaluated 3-...
Summary There is growing number of studies demonstrating a close relationship between insect gut microbiota and insecticide resistance. However, the contribution honey bee to host detoxification ability has yet be investigated. In order address this question, we compared expression cytochrome P450s (P450s) genes deficient (GD) workers conventional community (CV) mortality rates pesticide residue levels GD CV treated with thiacloprid or tau ‐fluvalinate. Our results showed that promotes P450...
Honey bee (Apis mellifera) colonies continue to experience high annual losses that remain poorly explained. Numerous interacting factors have been linked colony declines. Understanding the pathways linking pathophysiology with symptoms is an important step in understanding mechanisms of disease. In this study we examined specific pathologies associated honey bees collected from suffering Colony Collapse Disorder (CCD) and compared these apparently healthy colonies. We identified a set...