A5092184022- Peptidase Inhibition and Analysis
- S100 Proteins and Annexins
- Radiopharmaceutical Chemistry and Applications
- Ubiquitin and proteasome pathways
- Cellular Mechanics and Interactions
- Barrier Structure and Function Studies
- Cell Adhesion Molecules Research
- Cancer-related Molecular Pathways
- Cardiac Structural Anomalies and Repair
- Cancer Cells and Metastasis
- Biochemical and Molecular Research
Charles University
2023-2024
Bridge University
2024
Czech Academy of Sciences
2024
Military University Hospital Prague
2024
Masaryk University
2024
Brain metastases are a very common and serious complication of oncological diseases. Despite the vast progress in multimodality treatment, brain significantly decrease quality life prognosis patients. Therefore, identifying new targets microenvironment is desirable. Fibroblast activation protein (FAP) transmembrane serine protease typically expressed tumour-associated stromal cells. Due to its characteristic presence tumour microenvironment, FAP represents an attractive theranostic target...
T cells play a central role in cancer immunotherapy, yet the lack of standardized vitro models limits reproducibility and translational potential immune-cancer cell interaction studies. Existing often due to variability experimental conditions, immune sources, functional assays. To address this gap, we established robust reproducible co-culture model, which serves evaluate efficacy cell-based cytotoxicity using peripheral blood mononuclear (PBMCs) multiple lines. Healthy donor PBMCs...
Gliomagenesis induces profound changes in the composition of extracellular matrix (ECM) brain. In this study, we identified a cellular population responsible for increased deposition collagen I and fibronectin glioblastoma. Elevated levels fibrillar proteins were associated with expression fibroblast activation protein (FAP), which is predominantly found pericyte-like cells FAP+ present regions rich biopsy material produced substantially more vitro compared to other cell types GBM...
<title>Abstract</title> Purpose Gliomagenesis is associated with changes in extracellular matrix (ECM) composition. We investigate the role of fibroblast activation protein-positive (FAP+) pericyte-like cells ECM alterations glioblastoma and their impact on glioma cells. Methods Bioinformatic analysis, immunohistochemistry, ELISA were used to evaluate expression proteins FAP. FAP + isolated from human glioblastomas, production was quantified by using mass spectrometry analysis 3D matrices....
Abstract Background: Resistance to proteasome inhibitors (PI) bortezomib (BTZ) and carfilzomib (CFZ) is a major obstacle the successful treatment of multiple myeloma (MM); thus, identification novel therapeutic options an unmet medical need. ALK have been shown exhibit anti-MM activity in monotherapy or combination with CFZ. Our data showed that MM cells were negative for ALK; we aimed identify mechanism action inhibitor ceritinib synergy between Methods: A set PI-naïve PI-resistant was used...
Abstract BACKGROUND Brain metastases (BM) are a common complication of oncologic disease associated with dismal prognosis and there is an urgent need for novel therapeutic approaches. Fibroblast activation protein (FAP), transmembrane serine protease, emerging theranostic target in cancer due to its frequent upregulation the tumour microenvironment. FAP itself and/or FAP+ cells were shown promote progression several extracranial malignancies. However, very little known about expression role...