Abstract Metagenomic analysis typically includes read-based taxonomic profiling, assembly, and binning of metagenome-assembled genomes (MAGs). Here we integrate these steps in Read Annotation Tool (RAT), which uses robust signals from MAGs contigs to enhance read annotation. RAT reconstructs profiles with high precision sensitivity, outperforming other state-of-the-art tools. In high-diversity groundwater samples, annotates a large fraction the metagenomic reads, calling novel taxa at...

The biodegradation of organic aromatic compounds in subsurface environments is often hindered by limited dissolved oxygen. While oxygen supplementation can enhance situ biodegradation, it poses financial and technical challenges. This study explores introducing low-oxygen concentrations anaerobic for efficient contaminant removal, particularly scenarios where coexisting pollutants are present. An innovative strategy alternating nitrate-reducing microaerobic conditions to stimulate proposed,...

Groundwater is the main source for drinking water production globally. unfortunately can contain micropollutants (MPs) such as pesticides and/or pesticide metabolites. Biological remediation of MPs in groundwater requires an understanding natural biodegradation capacity and conditions required to stimulate activity. Thus, biostimulation experiments are a valuable tool assess field microorganisms. To this end, samples were collected at abstraction aquifer two locations, five different depths....

2,4-Dichlorophenoxyacetic acid (2,4-D) is the third most applied pesticide in Brazil to control broadleaf weeds crop cultivation and pastures. Due 2,4-D's high mobility long half-life under anoxic conditions, this herbicide has probability for groundwater contamination. Bioremediation an attractive solution 2,4-D contaminated environments, but there limited understanding of anaerobic biodegradation. In study, methanogenic enrichment cultures were obtained from Amazonian top soil (0-40 cm)...

BTEX (benzene, toluene, ethylbenzene, xylene) are common pollutants often found in former gasworks sites together with some other contaminants like indene, indane and naphthalene (Ie, Ia, N). This study aimed to evaluate the inhibitory or stimulative substrate interactions between BTEX, Ie, N during aerobic biodegradation. For this, batch bottles, containing originally anaerobic subsurface sediments, groundwater indigenous microorganisms from a contaminated site, were spiked various...

Groundwater quality is crucial for drinking water production, but groundwater resources are increasingly threatened by contamination with pesticides. As pesticides often occur at micropollutant concentrations, they unattractive carbon sources microorganisms and typically remain recalcitrant. Exploring microbial communities in aquifers used production an essential first step towards understanding the fate of micropollutants groundwater. In this study, we investigated interaction between...

Aromatic compounds persist as hazardous contaminants in both aquatic and terrestrial environments, needing rapid effective remediation strategies. This study evaluated toluene benzene biodegradation under sulfate nitrate-reducing conditions column experiments, utilizing aquifer sediments from a contaminated site. Over period of 36 weeks, four glass columns were operated simultaneously an alternating flow-batch regime. Each received either nitrate or electron acceptor while being exposed to...

BTEX (benzene, toluene, ethylbenzene, xylene) are common pollutants often found in former gaswork sites together with some other contaminants like indene, indane and naphthalene (Ie, Ia, N). This study aimed to evaluate the inhibitory or stimulative substrate interactions between BTEX, Ie, N during aerobic biodegradation. For this, batch bottles, containing originally anaerobic subsurface sediments, groundwater indigenous microorganisms coming from a contaminated site, were spiked various...

Metagenomic analysis typically includes read-based taxonomic profiling, assembly, and binning of metagenome-assembled genomes (MAGs). Here we integrate these steps in Read Annotation Tool (RAT), which uses robust signals from MAGs contigs to enhance read annotation. RAT reconstructs profiles with high precision sensitivity, outperforming other state-of-the-art tools. In high-diversity groundwater samples, annotates a large fraction the metagenomic reads, calling novel taxa at appropriate,...

Abstract Groundwater is a key water resource, with 45.7% of all drinking globally being extracted from groundwater. Maintaining good groundwater quality thus crucial to secure water. Micropollutants, such as pesticides, threaten which can be mitigated by biodegradation. Hence, exploring microbial communities in aquifers used for production essential understanding micropollutants biodegradation capacity. This study aimed at the interaction between geochemistry, pesticide presence, and...

Abstract Groundwater is the most important source for drinking water in The Netherlands. quality threatened by presence of pesticides, and biodegradation a natural process that can contribute to pesticide removal. conditions are oligotrophic thus be limited development microbial communities capable biodegrading pesticides. For reason, bioremediation technologies such as bioaugmentation (BA) help enhance biodegradation. We studied effect BA using enriched mixed inocula two column bioreactors...

Aromatic compounds persist as harmful contaminants in aquatic and terrestrial environments, necessitating efficient remediation. In this study, column experiments were conducted using aquifer sediments from a contaminated site to assess toluene benzene biodegradation under sulfate nitrate-reducing conditions. Four glass columns operated parallel over 36-week period, each containing distinct redox conditions substrate compositions supplied varying orders. A compound-specific, redox-condition...