A5091059899- Acute Lymphoblastic Leukemia research
- T-cell and Retrovirus Studies
- HIV/AIDS drug development and treatment
- Lymphoma Diagnosis and Treatment
- DNA and Nucleic Acid Chemistry
- Carcinogens and Genotoxicity Assessment
- Cancer Treatment and Pharmacology
- Monoclonal and Polyclonal Antibodies Research
- Cytomegalovirus and herpesvirus research
- Biochemical and Molecular Research
- Animal Genetics and Reproduction
- Synthesis and Characterization of Heterocyclic Compounds
- Click Chemistry and Applications
- RNA Interference and Gene Delivery
- DNA Repair Mechanisms
- Adenosine and Purinergic Signaling
- Childhood Cancer Survivors' Quality of Life
- Virus-based gene therapy research
- Viral-associated cancers and disorders
- Nitric Oxide and Endothelin Effects
- Neuropeptides and Animal Physiology
- Acute Myeloid Leukemia Research
- T-cell and B-cell Immunology
- Glycosylation and Glycoproteins Research
- Pneumocystis jirovecii pneumonia detection and treatment
Charles University
1999-2011
Czech Academy of Sciences, Institute of Molecular Genetics
2009
Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry
2001-2005
Czech Academy of Sciences
1995-1999
The University of Tokyo
1997
Institute of Pharmacology
1995
Research Institute for Pharmacy and Biochemistry (Czechia)
1992
Although polyomavirus JC (JCV) is the proven pathogen of progressive multifocal leukoencephalopathy, fatal demyelinating disease, this virus ubiquitous as a usually harmless symbiote among human beings. JCV propagates in adult kidney and excretes its progeny urine, from which DNA can readily be recovered. The main mode transmission parents to children through long cohabitation. In study, we collected substantial number urine samples native inhabitants 34 countries Europe, Africa, Asia. A...
Based on the powerful virostatic potency and cytostatic activity of adenine, 2,6-diaminopurine, guanine derivatives acyclic phosphonate nucleotide analog (S)-1-(3-hydroxy-2-phosphonomethoxypropyl) 9-(2-phosphonomethoxyethyl) series, we examined inhibitory potencies their diphosphates [(S)-9-(3-hydroxy-2-phosphonomethoxypropyl)adenine diphosphate (HPMPApp), 9-(2-phosphonomethoxyethyl)adenine diphosphate, 9-(2-phosphonomethoxyethyl)-2,6-diaminopurine (PMEDAPpp),...
Abstract BACKGROUND The translocation t(12;21)(p13;q22), which produces the TEL/AML1 fusion gene, is most frequent chromosomal abnormality in patients with childhood acute lymphoblastic leukemia (ALL) and generally associated a favorable prognosis. Furthermore, real‐time quantitative‐polymerase chain reaction (RQ‐PCR)‐based detection of represents an accurate technique for reproducible assessment minimal residual disease (MRD). METHODS authors employed RQ‐reverse transcriptase‐PCR...
ABSTRACT Tenofovir diphosphate (PMPApp) is a weak inhibitor of DNA polymerases (pol) α, δ, and ε*, with values for the K i PMPApp ( ) relative to m dATP 10.2, 15.2, respectively. Its incorporation into was about 1,000-fold less efficient than that dATP, 350-, 2,155-, 187-fold higher pol
The modulatory effect of FK 506 and cyclosporin A (CsA) on the expression inducible nitric oxide synthase (iNOS) in macrophages mechanisms their action were analysed. Isolated rat peritoneal cultured for 12 or 24 h with without lipopolysaccharide (LPS) (5 μg/ml) absence presence CsA (0.1 1 μg/ml). Total RNA from was isolated gene iNOS assessed by using RT-PCR. concentration NO2− culture supernatants taken as a measure (NO) production. reduced LPS-induced increase levels 68% 81%,...
OBJECTIVE--To investigate the formation of nitric oxide (NO) by peritoneal macrophages in three inbred strains rats differing both their susceptibility to induction adjuvant arthritis (AA) and severity disease. METHODS--AA was induced intraplantar injection Mycobacterium tuberculosis (M.tb) paraffin oil. Isolated were cultured for 24 hours supernatants assayed nitrite using Griess reagent. RESULTS--All LEW BN became diseased, but F344 strain included responders non-responders. No significant...
Using a single isolation procedure and selective assays for the determination of enzyme activity, highly purified DNA-polymerases α, δ ε were isolated from lymphoma Sprague-Dawley inbred rats. For pol α subunit composition was 170, 70, 57 53 kDa with sedimentation coefficient 8.7 S native molecule; delta consists two polypeptides (133 46 kDa; 8.2 S), while is polypeptide (140 kDa) its 7.0 S. Comparison interaction individual enzymes known inhibitors proliferating cell nuclear antigen (PCNA)...
O-Phosphonatomethylcholine, an isopolar phosphocholine analogue with a phosphonomethyl ether group replacing phosphomonoester residue, was prepared by reaction of diisopropyl 2-chloroethoxymethylphosphonate dimethylamine followed quaternization the thus-obtained 2-dimethylaminoethoxymethylphosphonate iodomethane; ester groups in quaternary intermediate were cleaved bromotrimethylsilane. Replacement sequence morpholine and/or pyrrolidine gave N-methylmorpholinium or N-methylpyrrolidinium...