A5077081921- Virus-based gene therapy research
- Poxvirus research and outbreaks
- Immunotherapy and Immune Responses
- Herpesvirus Infections and Treatments
- interferon and immune responses
- Animal Virus Infections Studies
- Immune Response and Inflammation
- Graphene and Nanomaterials Applications
- T-cell and B-cell Immunology
- Influenza Virus Research Studies
- Autophagy in Disease and Therapy
- Heat shock proteins research
- Viral gastroenteritis research and epidemiology
- Nanoparticles: synthesis and applications
- RNA regulation and disease
- Mosquito-borne diseases and control
- Immune Cell Function and Interaction
- vaccines and immunoinformatics approaches
- Brucella: diagnosis, epidemiology, treatment
- Gut microbiota and health
- Selenium in Biological Systems
- Carbon and Quantum Dots Applications
- Antimicrobial Peptides and Activities
- Infectious Diseases and Mycology
- Nail Diseases and Treatments
Warsaw University of Life Sciences
2015-2024
Ross University School of Veterinary Medicine
2020-2023
Public Health Dayton & Montgomery County
2010
The aim of the present study was to evaluate cytotoxicity pristine graphene monolayer and its utility as a scaffold for murine fibroblast L929 cell line. Cell viability, morphology, cytoskeleton architecture (microfilaments microtubules), adhesion migration into scratch-wound area were determined using graphene-coated microscopic slides. We found that fibroblasts cultured on exhibited changes in attachment, motility organization. Graphene have no increased proliferation within 24 h culture....
In tissue engineering, the possibility of a comprehensive restoration tissue, structure or portion organ is largely determined by type material used. A wide range materials such as graphene and other carbon nanocompounds which have different physical chemical properties can be expected to react differently upon contact with biomolecules, cells tissues. This mini-review describes current knowledge on biocompatibility its derivatives variety mammalian cells, osteoblasts, neuroendocrine...
Abstract Gastric and colorectal cancer are among the most frequently diagnosed malignancies of gastrointestinal tract. Searching for methods therapy that complements treatment or has a preventive effect is desirable. Bacterial metabolites safe human health, which have postbiotic effect, interest recently. The study aimed to preliminary assessment safety, antimicrobial, anti-cancer activity cell-free Gluconobacter oxydans strains isolated from Kombucha beverages as an example potential acetic...
This study investigates the effect of graphene scaffold on morphology, viability, cytoskeleton, focal contacts, mitochondrial network morphology and activity in BALB/3T3 fibroblasts provides new data biocompatibility “graphene-family nanomaterials”. We used monolayer applied onto glass cover slide by electrochemical delamination method regular slide, as a reference. The growing was unaltered, cell viability 95% compared to control cells non-coated slide. There no significant difference size...
Ectromelia virus (ECTV) is an orthopoxvirus responsible for mousepox, a lethal disease of certain strains mice that similar to smallpox in humans, caused by variola (VARV). ECTV, VARV, exhibits narrow host range and has co-evolved with its natural host. Consequently, ECTV employs sophisticated host-specific strategies control the immune cells are important induction antiviral response. In present study we investigated influence infection on functions murine GM-CSF-derived bone marrow...
Mitochondria are multifunctional organelles that participate in numerous processes response to viral infection, but they also a target for viruses. The aim of this study was define subcellular events leading alterations mitochondrial morphology and function during infection with ectromelia virus (ECTV). We used two different cell lines combination immunofluorescence techniques, confocal electron microscopy, flow cytometry address changes following infection. Early L929 fibroblasts RAW 264.7...
Several studies have provided evidence that complex relationships between autophagic and apoptotic cell death pathways occur in cancer virus-infected cells. Previously, we demonstrated infection of macrophages with Moscow strain ectromelia virus (ECTV-MOS) induces apoptosis under vitro vivo conditions. Here, found autophagy was induced RAW 264.7 cells during ECTV-MOS. Silencing beclin 1, an autophagy-related gene, reduced the percentage late macrophages. Pharmacological modulation by...
Cathepsins are a group of endosomal proteases present in many cells including dendritic (DCs). The activity cathepsins is regulated by their endogenous inhibitors – cystatins. crucial to antigen processing during viral and bacterial infections, as such prerequisite presentation the context major histocompatibility complex class I II molecules. Due involvement DCs both innate adaptive immune responses, quest understand impact poxvirus infection on host cells, we investigated influence...
Ectromelia virus (ECTV, the causative agent of mousepox), which represents same genus as variola (VARV, responsible for smallpox in humans), has served years a model studying mechanisms poxvirus-induced disease. Despite increasing knowledge on interaction between ECTV and its natural host-the mouse-surprisingly, still little is known about cell biology infection. Because pathogen with cytoskeleton growing area research virus-host interplay, aim present study was to evaluate consequences...
Ectromelia virus (ECTV) belongs to the Orthopoxvirus genus of Poxviridae family and is a natural pathogen mice. Certain strains mice are highly susceptible ECTV infection develop mousepox, lethal disease similar smallpox humans caused by variola virus. Currently, mousepox model one available small animal models for investigating pathogenesis generalized viral infections. Resistance susceptibility in controlled many genetic factors associated with multiple mechanisms immune response,...
Since smallpox vaccination was discontinued in 1980, there has been a resurgence of poxvirus infections, particularly the monkeypox virus. Without global recommendation to use vaccine, population is not immune, posing severe threat public health. Given these circumstances, it crucial understand relationship between poxviruses and their hosts. Therefore, this study focuses on ectromelia virus, causative agent mousepox, which serves as an excellent model for studying pathogenesis....
Mycophenolic acid (MPA) is an active metabolite of mycophenolate mofetil, a new immunosuppressive drug effective in the treatment canine autoimmune diseases. The impact MPA on immunity ambiguous and its influence immune system unknown. aim study was to determine markers changes stimulated peripheral lymphocytes after with vitro. Twenty nine healthy dogs were studied. Phenotypic functional analysis performed blood mononuclear cells cultured mitogens different concentrations– 1 μM (10−3...
We describe here a contribution of virus-induced actin tails and filopodia in transmission Ectromelia virus (ECTV) infection permissive cells detected by the immunofluorescence confocal microscopy. Immunoblot analysis revealed profoundly decreased beta-actin levels during ECTV replicative cycle infected 24 hrs post (p.i.). These results provided basis for further motion as well impact on cytoskeletal proteins.
Mitochondria are extremely important organelles in the life of a cell. Recent studies indicate that mitochondria also play fundamental role cellular innate immune mechanisms against viral infections. Moreover, able to alter their shape continuously through fusion and fission. These tightly regulated processes activated or inhibited under physiological pathological (e.g. infection) conditions help restore homeostasis. However, many types viruses, such as orthopoxviruses, have developed...
A microbioanalytical method of stem cell (SC) differentiation towards cardiac cells using a newly developed digitally controlled microdispenser.