A5037906104- Parkinson's Disease Mechanisms and Treatments
- Metabolism and Genetic Disorders
- Alzheimer's disease research and treatments
- Glycosylation and Glycoproteins Research
- Enzyme Structure and Function
- Epigenetics and DNA Methylation
- Cancer, Hypoxia, and Metabolism
- Folate and B Vitamins Research
- RNA and protein synthesis mechanisms
- Pancreatic function and diabetes
- Botulinum Toxin and Related Neurological Disorders
- RNA modifications and cancer
- Monoclonal and Polyclonal Antibodies Research
- Diabetes and associated disorders
- Computational Drug Discovery Methods
- Cellular transport and secretion
- Biochemical and Molecular Research
- Prion Diseases and Protein Misfolding
- Spectroscopy Techniques in Biomedical and Chemical Research
- Heat shock proteins research
- Neurological disorders and treatments
- Genetic Neurodegenerative Diseases
- Genomics and Chromatin Dynamics
- Tuberculosis Research and Epidemiology
- RNA Research and Splicing
Qatar Foundation
2019-2024
Hamad bin Khalifa University
2019-2024
Indian Institute of Science Education and Research, Bhopal
2020-2024
Brazilian Center for Research in Energy and Materials
2017-2023
Brazilian Biosciences National Laboratory
2017-2023
Laboratório Nacional de Ciência e Tecnologia do Bioetanol
2017-2022
Council of Scientific and Industrial Research
2009-2015
The active transport of glycolytic pyruvate across the inner mitochondrial membrane is thought to involve two carrier subunits, MPC1 and MPC2, assembled as a 150 kDa heterotypic oligomer. Here, recombinant production human MPC through co-expression strategy first described; however, substantial complex formation was not observed, predominantly individual subunits were purified. In contrast MPC1, which co-purifies with host chaperone, we demonstrated that MPC2 homo-oligomers promote efficient...
Abstract Background PHF21A has been associated with intellectual disability and craniofacial anomalies based on its deletion in the Potocki-Shaffer syndrome region at 11p11.2 disruption three patients balanced translocations. In addition, de novo truncating mutations were reported recently. Here, we analyze genomic data from seven unrelated individuals provide detailed clinical descriptions, further expanding phenotype haploinsufficiency. Methods Diagnostic trio whole exome sequencing,...
X-ray crystallography has facilitated the majority of protein structures determined to date. Sequence-based predictors that can accurately estimate crystallization propensities would be highly beneficial overcome high expenditure, large attrition rate, and reduce trial-and-error settings required for crystallization.In this study, we present a novel model, BCrystal, which uses an optimized gradient boosting machine (XGBoost) on sequence, structural physio-chemical features extracted from...
Nanobodies (Nbs), the single‐domain antigen‐binding fragments of dromedary heavy‐chain antibodies (HCAb), are excellent candidates as therapeutic and diagnostic tools in synucleinopathies because their small size, solubility stability. Here, we constructed an immune nanobody library specific to monomeric form alpha‐synuclein (α‐syn). Phage display screening allowed identification a nanobody, Nbα‐syn01, for α‐syn. Unlike previously developed nanobodies, Nbα‐syn01 recognized N‐terminal region...
Amyloid fibrillation is the root cause of several neuro as well non-neurological disorders. Understanding molecular basis amyloid aggregate formation crucial for deciphering various neurodegenerative diseases. In our study, we have examined lysozyme process using nano-infrared spectroscopy (nanoIR). NanoIR enabled us to investigate both structural and chemical characteristics fibrillar species concurrently. The spectroscopic results indicate that transformed into a structure having mainly...
The timing of host cell lysis is a fundamental life history parameter in bacteriophages as it presents an evolutionary trade-off between maximizing intracellular phage replication and optimizing transmission to new cells, ultimately determining viral fitness dynamic bacterial populations. In the bacteriophage λ, time dependent on expression timekeeper protein, holin, which accumulates Escherichia coli inner membrane. Cell triggered when membrane concentration holin crosses critical threshold...
The riboflavin biosynthesis pathway has been shown to be essential in many pathogens and is absent humans. Therefore, enzymes involved synthesis are considered as potential antibacterial drug targets. enzyme 3,4-dihydroxy-2-butanone-4-phosphate synthase (DHBPS) catalyzes one of the two committed steps converts d-ribulose 5-phosphate (Ru5P) l-3,4-dihydroxy-2-butanone 4-phosphate formate. Moreover, DHBPS indispensable for Mycobacterium, Salmonella, Helicobacter species. Despite essentiality...
The prevalence of homocystinuria in Qatar is 1:1800, mainly due to a founder missense mutation p.R336C. cystathionine beta-synthase (CBS) R336C mutant was bacterially expressed, purified and its molecular properties were compared CBS wild type (WT) recombinant protein. Our data revealed that p.R336C results dramatic reduction (∼86%) enzymatic activity. Circular Dichroism experiments suggested the does not significantly alter secondary structure CD spectra also distinct differences thermal...
Abstract Motivation A global effort is underway to identify compounds for the treatment of COVID-19. Since de novo compound design an extremely long, time-consuming and expensive process, efforts are discover existing that can be repurposed COVID-19 new viral diseases. We propose a machine learning representation framework uses deep induced vector embeddings proteins as features predict compound-viral protein activity. The prediction model in-turn consensus rank approved against interest....
The need for the early detection of emerging pathogenic viruses and their newer variants has driven urgent demand developing point-of-care diagnostic tools. Although nucleic acid-based methods such as reverse transcription-quantitative polymerase chain reaction (RT-qPCR) loop-mediated isothermal amplification (LAMP) have been developed, a more facile robust platform is still required. To address this need, proof-of-principle study, we engineered prototype—the versatile, sensitive, rapid,...
J-domain proteins (JDPs) are obligate cochaperones of Hsp70s with a wide range functions in protein homeostasis. Although the is required for stimulation ATPase activity, functional specificity JDPs governed by domains or regions other than J-domain. Jjj3/Dph4, class III JDP, diphthamide (DPH) biosynthesis eukaryotes, including yeast and mammals. Dph4 has conserved N-terminal an uncharacterized C-terminal domain containing signature CSL zinc finger motif. Previously, we showed that ortholog