A5018577391- Bone health and osteoporosis research
- Sex and Gender in Healthcare
- Topological and Geometric Data Analysis
- Bone Tissue Engineering Materials
- Medical Imaging and Analysis
- Mesenchymal stem cell research
- Cell Image Analysis Techniques
- Leprosy Research and Treatment
- Spine and Intervertebral Disc Pathology
- Bone Metabolism and Diseases
- Connective tissue disorders research
- Extracellular vesicles in disease
- AI in cancer detection
- Medical Imaging Techniques and Applications
- RNA Interference and Gene Delivery
- Vitamin D Research Studies
- Scoliosis diagnosis and treatment
- Electrospun Nanofibers in Biomedical Applications
- MicroRNA in disease regulation
- Bone health and treatments
- Cancer-related molecular mechanisms research
- Diversity and Career in Medicine
- Virus-based gene therapy research
- Graphene and Nanomaterials Applications
- RNA Research and Splicing
University College London
2023-2024
Royal Veterinary College
2024
University of Southampton
2019-2023
ABSTRACT The optimal biomechanical function of the spine depends on hierarchical structures spanning whole joint to molecular scales. vertebral endplates experience complex, location specific loading from intervertebral discs, and their behaviour is governed by microarchitecture, mineralisation, prestrain constituent bone cartilage. Here we use a combination synchrotron X-ray tomography, digital volume correlation, wide-angle diffraction investigate relationships between microstructure...
ABSTRACT Osteoblast (OB) lineage cells are an important source of vascular endothelial growth factor (VEGF), which is critical for bone and repair. During development, pubertal differences in males females exist, but little known about whether VEGF signaling contributes to skeletal sexual dimorphism. We have found that mice, conditional disruption osteocalcin-expressing (OcnVEGFKO) exerts a divergent influence on morphological, cellular, whole properties between sexes. Furthermore, we...
Despite knowledge that sexually dimorphic mechanisms regulate bone homeostasis, sex often remains unreported and unconsidered in preclinical experimental design. Failure to report could lead inappropriate generalizations of research findings less effective translation into clinical practice. Preclinical bias (preferential selection one sex) is present across other fields, including neuroscience immunology, but uninvestigated skeletal research. For context, we first summarized key literature...
Abstract We present a topological method for the detection and quantification of bone microstructure from non-linear microscopy images. Specifically, we analyse second harmonic generation (SHG) two photon excited autofluorescence (TPaF) images tissue which capture distribution matrix (fibrillar collagen) structure autofluorescent molecules, respectively. Using persistent homology statistics with signed Euclidean distance transform filtration on binary patches images, are able to quantify...
Mineralization of bone is achieved by the sequential maturation immature amorphous calcium phase to mature hydroxyapatite (HA) and central in process development repair. To study normal dysregulated mineralization vitro, substrates are often coated with poly-l-lysine (PLL) which facilitates cell attachment. This has used Raman spectroscopy investigate effect PLL coating on osteoblast (OB) matrix composition during differentiation, a focus collagen specific proline hydroxyproline precursors...
Collagen assembly during development is essential for successful matrix mineralisation, which determines bone quality and mechanocompetence. However, the biochemical structural perturbations that drive pathological skeletal collagen configuration remain unclear. Deletion of vascular endothelial growth factor (VEGF; also known as VEGFA) in bone-forming osteoblasts (OBs) induces sex-specific alterations extracellular (ECM) conformation mineralisation coupled to changes, are augmented males....
Abstract Lower back pain is linked to vertebral biomechanics, with endplates (VEPs) playing a key role in load transfer and distribution. Synchrotron computed tomography (sCT) allows for detailed visualisation of the microstructure intact VEPs under near-physiological loads and, when coupled digital volume correlation (DVC), can be used quantify three-dimensional (3D) strain fields nanoscale resolution. Herein, we spatially couple DVC data an image-based finite element model (FEM) determine...
Introduction Chondrocytes are continuously exposed to loads placed upon them. Physiological pivotal the maintenance of articular cartilage health, while abnormal contribute pathological joint degradation. Similarly, growth plate is subject various during and development. Due high-water content cartilage, hydrostatic pressure considered one main biomechanical influencers on chondrocytes has been shown play an important role in mechano-regulation cartilage. Methods Herein, we conducted RNAseq...
ABSTRACT We present a topological method for the detection and quantification of bone microstructure from non-linear microscopy images. Specifically, we analyse second harmonic generation (SHG) two photon excited autofluorescence (TPaF) images tissue which capture distribution matrix (fibrillar collagen) structure autofluorescent molecules, respectively. Using persistent homology statistics with signed Euclidean distance transform filtration on binary patches images, are able to quantify...