A5100747415- Monoclonal and Polyclonal Antibodies Research
- Lung Cancer Diagnosis and Treatment
- Immune Cell Function and Interaction
- Bacteriophages and microbial interactions
- Radiomics and Machine Learning in Medical Imaging
- Ophthalmology and Eye Disorders
- Ginger and Zingiberaceae research
- Viral Infectious Diseases and Gene Expression in Insects
- Cancer Immunotherapy and Biomarkers
- T-cell and B-cell Immunology
Pusan National University Yangsan Hospital
2015
Traditional hybridoma and B cell cloning antibody discovery platforms have inherent limits in immune repertoire sampling depth. One consequence is that monoclonal (mAb) leads often lack the necessary affinity for therapeutic applications, thus requiring labor-intensive time-consuming vitro engineering optimization steps. Here, we show high-affinity variants of mouse-derived mAbs can be rapidly obtained by testing somatic sequence deep sequencing variable regions repertories from immunized...
Since the invention of Hybridoma technology by Milstein and Köhler in 1975, its application has greatly advanced antibody discovery process. The enables both functional screening long-term archival immortalized monoclonal producing B cells. Despite dependable cryopreservation for hybridoma cells, practicality storage been outpaced recent progress robotics automations, which routine identification thousands antigen specific clones. Such throughput increase imposes two nascent challenges...
Zerumbone is the main ingredient extracted from wild ginger Zingiber zermbet Smith. It known that root of contains most zerumbone. has been reported to function in several biological environments, including tumors and inflammation. In addition, it affect functions types cells, immune cells. this study, was confirmed migration phenomenon induced by SDF-1α inhibited zerumbone using T cells isolated mouse spleen. On other hand, proliferation through antiCD3 anti-CD28 antibodies not affected...