All known virulence genes of Listeria monocytogenes are under positive regulation by the transcription factor PrfA. Previous work employing L. strain NCTC7973 suggested that disaccharide cellobiose might serve as a specific ‘signature molecule’ which functions to prevent activation PrfA‐controlled regulon in soil environment. We have examined three other strains, 10403S, LO28 and EGD, all commonly regarded wild‐type isolates, find is anomalous with respect effect carbohydrates on expression...

The spo0A genes of Clostridium beijerinckii NCIMB 8052 and cellulolyticum ATCC 35319 were isolated characterized. C‐terminal DNA‐binding domains the predicted products from these two organisms, as well 16 other taxonomically diverse species Bacillus , show extensive amino acid sequence conservation (56% identity, 65% similarity over 104 residues). A 12‐amino‐acid motif (SRVERAIRHAIE) that forms putative DNA recognition helix is particularly highly conserved, suggesting a common target....

Functioning of the spoIIE locus Bacillus subtilis is required for formation a normal polar septum during sporulation and activation transcription factor σ F , which directs early forespore‐specific gene expression. We have determined DNA sequence wild type several mutant alleles B. sequenced substantial portion its presumptive homologue in megaterium . show that encodes single large protein with predicted molecular mass 92 kDa. Each five point‐mutation alleles, traditionally defined locus,...

New vectors were constructed for efficient transposon Tn917-mediated mutagenesis of poorly transformable strains Streptococcus mutans(pTV1-OK) and subsequent recovery interrupted genes in Escherichia coli (pT21delta2TetM). In this report, we demonstrate the utility Tn917 a strain S. mutans (JH1005) by showing (i) conditional replication pTV1-OK, repA(Ts) derivative broad-host-range plasmid pWVO1 harboring Tn9l7, JH1005 at permissive temperature (30 degrees C) versus that nonpermissive (45...

Between

Summary Spo0A is a phosphorylation‐activated transcription factor of Bacillus subtilis. It member the response regulator super‐family bacterial signal transduction proteins and controls many changes in gene expression that occur during transition into stationary phase initiation speculation. To identify domains most critical for determining its structural functional features, presumptive homologues spo0A were characterized collection eight species six Clostridium representing...

The kinetics of formate metabolism in Methanobacterium formicicum and Methanospirillum hungatei were studied with log-phase formate-grown cultures. progress degradation was followed by the formyltetrahydrofolate synthetase assay for fitted to integrated form Michaelis-Menten equation. K m V max values 0.58 mM 0.037 mol h −1 g (dry weight), respectively. lowest concentration metabolized 26 μM. 0.22 0.044 15 apparent dehydrogenase cell-free extracts 0.11 mM. H 2 uptake cultures 6 μM dissolved...

The 18.1-kilobase plasmid pSE211 integrates into the chromosome of Saccharopolyspora erythraea at a specific attB site. Restriction analysis integrated plasmid, pSE211int, and adjacent chromosomal sequences allowed identification attP, attachment Nucleotide sequencing attB, attL, attR revealed 57-base-pair sequence common to all sites with no duplications or in state, indicating that integration takes place through conservative, reciprocal strand exchange. An indicated presence putative gene...

The molecular structure of S2Cl2 has been determined accurately by electron diffraction. ClSSCl chain configuration the dimensions: S—Cl = 2.057 ± 0.002 Å, S—S 1.931 0.005 SSCl 108.2 0.3°, ϕ(the dihedral angle between two planes)= 84.8 1.3°. bond distances occur within a single peak radial distribution curve; resulting correlation problem was solved carrying out refinements with constrained amplitudes. study confirms absence pyramidal isomer S SCl2, but existence SClClS cannot be ruled...

A cohort of 1,708 dry-cleaning workers identified from union records was exposed to perchloroethylene (PCE), a known animal carcinogen and probable human carcinogen, for at least 1 year before 1960. Many also had exposure Stoddard solvent, petroleum-based solvent.Vital status updated through 1996 life table analyses conducted.The excess cancer mortality (271 deaths, standardized ratio [SMR] 1.25, 95% confidence interval [CI] 1.11-1.41). Elevated SMRs tongue, bladder, esophagus, intestine,...

Copper (II) acetylacetonate (Cu(acac)2) vapor decomposition and subsequent copper (I) oxide particle formation were studied in a vertical laminar flow reactor the presence of hydrogen water nitrogen atmosphere. The does not significantly affect on rate. most reactive conditions for precursor appear when is introduced into system. A mechanism Cu(acac)2 has been proposed. reaction pathway can be divided three steps: gaseous hydrate complex; proton transition from coordinated to ligand further...

The mortality in a cohort of workers at vinyl chloride polymerization plant has been updated, extending the period observation original study from 1974 to 1986. Workers this may have exposed monomer and/or polyvinyl dust, or had no exposure either substance. Seventy-six percent work force worked jobs with potential monomer. Among total cohort, statistically significant excess risks were observed for liver, lung, and brain cancer. For subcohort monomer, standardized ratio (SMR) liver cancer...

Mortality patterns were studied in 1,165 workers exposed to sulfuric acid mist and other mists (primarily hydrochloric mist) steel-pickling operations. Standardized mortality ratio (SMR) analysis of the full “any exposure” cohort (n = 1,165), with use U.S. death rates as a standard, showed that lung cancer was significantly elevated, 1.64 [95% confidence interval (CI) 1.14–2.28, based on 35 observed deaths]. The for only (n=722) lower (SMR 1.39), but further restriction time 20 years more...

An 11.3-kilobase-pair plasmid, designated pSE101, exists in Saccharopolyspora erythraea NRRL 2338 as an integrated sequence (pSE101int) at a unique chromosomal location and the free form less than average of 1 copy per 10 chromosomes. The plasmid is missing from S. 2359. Restriction maps forms pSE101 showed point-to-point correspondence. Plasmid pECT2 was constructed by ligation pBR322, gene for thiostrepton resistance (tsr). When introduced polyethylene glycol-mediated transformation into...

A previous mortality study among four organochlorine pesticide manufacturers was updated through 1987. The pesticides included chlordane; heptachlor and endrin; aldrin, dieldrin dichlorodiphenyltrichloroethane. for all causes malignant neoplasms at each of the plants lower than expected. There a statistically significant increase in liver biliary tract cancer workers plant 3 (5 observed, standardized ratio 393, 95% confidence interval 1.27-9.20). These results are somewhat consistent with...

Crystalline nanometer-sized Cu 2 O and CuO particle formation was studied by vapor thermal decomposition of copper (II) acetylacetonate in a vertical laminar flow reactor at ambient pressure. Experiments were carried out 3 furnace temperature profiles (maximum values t furn = 432, 596, 705°C) with carrier gases (oxygen/nitrogen mixture ratios 0.5/99.5 10.0/90.0). The results computational fluid dynamics simulations are presented. introduction oxygen into the system found to increase rate...