A5027057580- Acute Myeloid Leukemia Research
- Histone Deacetylase Inhibitors Research
- Epigenetics and DNA Methylation
- Viral Infectious Diseases and Gene Expression in Insects
- Protein Degradation and Inhibitors
- Virus-based gene therapy research
- Developmental Biology and Gene Regulation
- CAR-T cell therapy research
- Genomics and Chromatin Dynamics
- Cancer-related gene regulation
- RNA Interference and Gene Delivery
- T-cell and B-cell Immunology
- Glycosylation and Glycoproteins Research
- Hematopoietic Stem Cell Transplantation
- Cytomegalovirus and herpesvirus research
- interferon and immune responses
- Viral Infections and Immunology Research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Systemic Lupus Erythematosus Research
- Molecular Biology Techniques and Applications
- Cervical Cancer and HPV Research
- Acute Lymphoblastic Leukemia research
- Cytokine Signaling Pathways and Interactions
- Urological Disorders and Treatments
- Cancer Genomics and Diagnostics
King's College London
2015-2021
London Cancer
2015
Cardiff University
2015
University College London
2014-2015
CRUK Lung Cancer Centre of Excellence
2014
Queen's University Belfast
2009-2013
Royal Holloway University of London
2000
A number of epigenetic alterations occur in both the virus and host cellular genomes during human papillomavirus (HPV)-associated carcinogenesis, investigations such alterations, including changes chromatin proteins histone modifications, have potential to lead therapeutic reversion. We report here that transformed HPV16 E6/E7-expressing primary foreskin keratinocytes (HFKs) (E6/E7 cells) demonstrate increased expression PRC2 methyltransferase EZH2 at mRNA protein levels but do not exhibit...
To evaluate the impact of DNMT3A mutations on outcome in younger patients with cytogenetic intermediate-risk acute myeloid leukemia.
The cytogenetically normal subtype of acute myeloid leukemia is associated with an intermediate risk which complicates therapeutic options. Lower overall HOX/TALE expression appears to correlate more favorable prognosis/better response treatment in some leukemias and solid cancer. functional significance the gene chemotherapy not known. Three independent microarray datasets obtained from large cohorts patients along quantitative polymerase chain reaction validation were used identify a...
The incidence of refractory acute myeloid leukemia (AML) is on the increase due in part to an aging population that fails respond traditional therapies. High throughput genomic analysis promises better diagnosis, prognosis, and therapeutic intervention based improved patient stratification. Relevant preclinical models are urgently required advance drug development this area. collaborating oncogenes, HOXA9 MEIS1, frequently co-overexpressed cytogenetically normal AML (CN-AML), a conditional...
Lentiviral vectors (LVs) have recently witnessed an increasing demand in research and clinical applications. Their current purification processes represent the main bottleneck their widespread use, as methods used are cumbersome yield low recoveries. We aimed to develop a one-step method specifically purify LVs, with high yields reduced levels of impurities, using biotin-streptavidin system. Herein, packaging HEK293T cells were genetically engineered cyclical biotin-mimicking peptide...
Summary Older adult patients (≥60 years) with acute myeloid leukaemia ( AML ) are generally considered to be poor‐risk and there is limited information available regarding risk stratification based on molecular characterization in this age group, particularly for the double‐mutant CEBPA DM genotype. To investigate whether a favourable‐risk genotype can identified, we investigated , NPM1 FLT3 status prognostic impact cohort of 301 aged 60 years or more intermediate‐risk cytogenetics, all...
The Hox family are master transcriptional regulators of developmental processes, including hematopoiesis. regulators, caudal homeobox factors (Cdx1-4), and Meis1, along with several individual proteins, implicated in stem cell expansion during embryonic development, gene dosage playing a significant role the overall function integrated network. To investigate this network normal aberrant, early hematopoiesis, we employed an vitro differentiation system, which recapitulates mouse Expression...
High expression of the HOXA cluster correlates with poor clinical outcome in acute myeloid leukemias, particularly those harboring rearrangements mixed-lineage-leukemia gene (MLLr). Whilst decreased acts as a readout for candidate experimental therapies, necessity leukemia maintenance has not been fully explored. Primary leukemias were generated hematopoietic stem/progenitor cells from Cre responsive transgenic mice conditional deletion Hoxa locus. resulted reduced proliferation and colony...