Objective The purpose of this study was to delineate the cellular, mechanical and morphometric effects altered loading on mandibular condylar cartilage (MCC) subchondral bone. We hypothesized that will induce differentiation cells by accelerating lineage progression MCC. Materials Methods Four-week-old male Dkk3 XCol2A1XCol10A1 mice were randomly divided into two groups: (1) Loaded-Altered MCC induced forced mouth opening using a custom-made spring; (2) Control-served as an unloaded group....

Estrogen protects females from hepatocellular carcinoma (HCC). To determine whether this protection is mediated by classic estrogen receptors, we tested HCC susceptibility in receptor-deficient mice. In contrast to a previous study, found that diethylnitrosamine induces hepatocarcinogenesis significantly greater extent when lack Esr1, which encodes Receptor-α. Relative wild-type littermates, Esr1 knockout developed 9-fold more tumors. Deficiency of Esr2, Receptor-β, did not affect liver...

Human saliva has been identified as a novel, practical, and noninvasive source of biomarkers genetic materials. However, it is equally challenging due to the availability an abundance impurities in form microbes other proteinaceous compounds. The objective this study was develop robust, reproducible, economic method extracting high-yield high-quality RNA from whole human saliva.The modified TRIzol protocol developed extract (n = 14), followed by complementary DNA synthesis reverse...

Objective Bone morphogenetic protein 2 (BMP2) plays important roles in cartilage growth and development. Paradoxically, elevated levels of BMP2 leads to hypertrophic differentiation osteoarthritis cartilage. We examined the vivo loss cells expressing aggrecan mandibular condyle knee. Design Three-week-old flox/flox- CreER-positive mice their Cre-negative littermates were treated with tamoxifen raised until 3 or 6 months. also investigated direct effects on chondrocytes vitro. Cells from...

Structured Abstract Objective To study the effects of intermittent parathyroid hormone ( PTH [1‐34]) on mandibular condylar cartilage MCC ) and subchondral bone in adult female mice. Materials Methods Twenty‐two, 20‐week‐old mice were used for vivo experiments. The experimental (n=11) received daily intraperitoneal injections [1‐34] 3 weeks, while control (n = 11) 0.9% saline solution. Mice euthanized then micro‐computed tomography (micro‐ CT ); histology immunostaining carried out to assess...

The temporomandibular joint (TMJ) has the capacity to adapt external stimuli, and loading changes can affect position of condyles, as well structural cellular components mandibular condylar cartilage (MCC). This manuscript describes methods for analyzing these a method altering TMJ in mice (i.e., compressive static loading). evaluation illustrated here is simple morphometric approach that uses Digimizer software performed radiographs small bones. In addition, analysis leading alterations...

Objective To characterize the long-term effects of intermittent parathyroid hormone (I-PTH) on mandibular condylar cartilage (MCC) and subchondral bone temporomandibular joint, in vivo vitro. Materials Methods For experiments, sixteen 10-week-old mice were divided into 2 groups: (1) I-PTH ( n = 8)—subcutaneous daily injection PTH; (2) control group saline solution. Experiments carried out for 4 weeks. Mice injected with calcein, alizarin complexone, cell proliferation marker before...

The temporomandibular joint (TMJ) has the capacity to adapt external stimuli, and loading changes can affect position of condyles, as well structural cellular components mandibular condylar cartilage (MCC). This manuscript describes methods for analyzing these a method altering TMJ in mice (i.e., compressive static loading). evaluation illustrated here is simple morphometric approach that uses Digimizer software performed radiographs small bones. In addition, analysis leading alterations...

Objective To characterize the effects of parathyroid hormone (PTH) and alendronate (Alend) on osteochondral tissue temporomandibular joint (TMJ). Materials Methods Ninety-six male female transgenic reporter mice, 4 to 5 weeks old were divided into 6 groups: (1) Control group: Saline was injected daily for 14 days; (2) PTH: PTH (3) Alend: Alend every alternate days (4) Combined (5) then followed by injections in (6) wait days. There a waiting period 1 week before administration Mice with...

Abstract The Massachusetts Military Reservation (MMR) is a Superfund site where ground water has been contaminated by mixture of pollutants. Exposure to these chemicals public health concern and reproductive impairments have observed in population turtles (Chrysemys picta) endemic this site. We hypothesize that developmental exposure endocrine disrupting compounds originating from the MMR might lead abnormalities seen adult animals. Upon examination egg yolk at impacted site, we found...

In this study, we aimed to clone brain-derived Cyp19b and ovary-derived Cyp19a the P450 aromatase gene isoforms indicate expression levels of these genes in hypothalamus ovary tissues from reproductively arrested ovarian development (RA) non-arrested (RN) Alburnus tarichi Lake Van, Turkey. The were predominant ovary, respectively. level mRNA was significantly lower RA fish than RN (P<0.05). (P<0.05) while no difference found both According findings, suggest that represent a segment...