A5086350685- Endoplasmic Reticulum Stress and Disease
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Machine Learning in Bioinformatics
- Cellular transport and secretion
- Mitochondrial Function and Pathology
- Adipose Tissue and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Folate and B Vitamins Research
- Transgenic Plants and Applications
- Marine and coastal plant biology
- Advanced Proteomics Techniques and Applications
- Protist diversity and phylogeny
- Porphyrin Metabolism and Disorders
- RNA Research and Splicing
- Bioinformatics and Genomic Networks
- Algal biology and biofuel production
- Biochemical and Molecular Research
- ATP Synthase and ATPases Research
- Metabolism and Genetic Disorders
- Pancreatic function and diabetes
University of Geneva
2008-2021
Geneva College
2020
SIB Swiss Institute of Bioinformatics
2012-2020
Centre National de la Recherche Scientifique
2006
Sorbonne Université
2006
Abstract The emergence of small open reading frame (sORF)-encoded peptides (SEPs) is rapidly expanding the known proteome at lower end size distribution. Here, we show that mitochondrial proteome, particularly respiratory chain, enriched for proteins. Using a prediction and validation pipeline SEPs, report discovery 16 endogenous nuclear encoded, mitochondrial-localized SEPs (mito-SEPs). Through functional prediction, proteomics, metabolomics metabolic flux modeling, demonstrate BRAWNIN, 71...
Mammalian cells secrete a large number of small proteins, but their mode translocation into the endoplasmic reticulum is not fully understood. Cotranslational was expected to be inefficient due time window for signal sequence recognition by particle (SRP). Impairing SRP pathway and reducing cellular levels translocon component Sec62 RNA interference, we found an alternate, Sec62-dependent path in mammalian required efficient proteins with N-terminal sequences. The occurs posttranslationally...
The signal recognition particle (SRP) is a ubiquitous cytoplasmic ribonucleoprotein complex required for the cotranslational targeting of proteins to endoplasmic reticulum (ER). In eukaryotes, SRP has arrest elongation nascent chains during ensure efficient translocation preprotein, and this function dependent on SRP9/14. Here we present results mutational study human protein h9/14 that identified characterized regions single residues essential activity. Effects mutations were assessed both...
The methionine salvage pathway is widely distributed among some eubacteria, yeast, plants and animals recycles the sulfur-containing metabolite 5-methylthioadenosine (MTA) to methionine. In eukaryotic cells, takes place in cytosol usually involves six enzymatic activities: MTA phosphorylase (MTAP, EC 2.4.2.28), 5′-methylthioribose-1-phosphate isomerase (mtnA, 5.3.1.23), 5′-methylthioribulose-1-phosphate dehydratase (mtnB, EC: 4.2.1.109), 2,3-dioxomethiopentane-1-phosphate enolase/phosphatase...
The signal recognition particle (SRP) is a conserved ribonucleoprotein that targets membrane and secreted proteins to translocation channels in membranes. In eukaryotes, the Alu domain, which comprises 5′ 3′ extremities of SRP RNA bound SRP9/14 heterodimer, thought interact with ribosome pause translation elongation during docking. We present 3.2 Å resolution crystal structure chimeric comprising from archaeon Pyrococcus horikoshii human binding SRP9/14. reveals how intricate tertiary...
Abstract About 10% of human proteins have no annotated function in protein knowledge bases. A workflow to generate hypotheses for the these uncharacterized has been developed, based on predicted and experimental information properties, interactions, tissular expression, subcellular localization, conservation other organisms, as well phenotypic data mutant model organisms. This applied seven (C6orf118, C7orf25, CXorf58, RSRP1, SMLR1, TMEM53 TMEM232) frame a course-based undergraduate research...
Abstract The emergence of small open reading frame (sORF)-encoded peptides (SEPs) is rapidly expanding the known proteome at lower end size distribution 1,2 . Here, we show that mitochondria enriched for proteins smaller than 100 a.a. (defined as SEPs). Using a mitochondrial prediction and validation pipeline open-reading-frame (SEPs), report discovery 16 endogenous SEPs (mito-SEPs) associated with oxidative phosphorylation (OXPHOS). Through functional prediction, proteomics, metabolomics...