Cyclophilin A has been found to be involved in many inflammatory diseases via its receptor, cluster of differentiation 147 (CD147). This study was designed estimate the potential role cyclophilin A/CD147 subarachnoid hemorrhage-induced early brain injury.Controlled vivo laboratory study.Animal research laboratory.Two hundred ninety adult male Sprague-Dawley rats weighing 300-350 g.A prechiasmatic cistern single-injection model used produce experimental hemorrhage rats. The expressions and...

The protein kinase R (PKR)-like endoplasmic reticulum (PERK) signaling pathway was reported to exert an important role in neuronal apoptosis. present study designed investigate the roles of PERK secondary brain injury (SBI) induced by intracerebral hemorrhage (ICH) and its potential mechanisms. Sprague-Dawley rats were used establish ICH models injecting autologous blood (100 μl), cultured primary rat cortical neurons exposed oxyhemoglobin (10 μM) mimic vitro. antagonist, GSK2606414,...

Surgical brain injury (SBI) triggers microglia to release numerous inflammatory factors, leading edema and neurological dysfunction. Reducing neuroinflammation protecting the blood-brain barrier (BBB) are key factors improve function prognosis after SBI. Na + -K -Cl – cotransporter 1 (NKCC1) nuclear factor κB (NF-κB) have been implicated in secretion of cytokines by injury. This study aimed establish role NKCC1 inducing inflammation SBI, as well determine whether controls interleukin-1β...

Leucine-rich repeat kinase 2 (LRRK2) is widely expressed in the brain and exerts neurotoxicity Parkinson's disease. The p38/Drosha signaling activation has been reported to increase cell death under stress. This study was designed investigate potential role mechanism of LRRK2 secondary injury after traumatic (TBI). A total 130 male Sprague-Dawley rats were examined using a weight-drop model TBI. received specific inhibitor PF-06447475 or pDNA alone combination with Drosha pDNA. Real-time...

Blood-brain barrier (BBB) damage is closely related to morbidity and mortality in patients with traumatic brain injury (TBI). Inhibition of VEGF effectively protects BBB integrity clinical ischemic stroke. Protecting integrity, reducing edema alleviating post-TBI secondary are key a favorable patient prognosis. MMP-9 affects by destroying the tight junction vascular endothelial cells inhibiting transport enzymatic systems. The present study aimed examine possible interplay between TBI. A TBI...

Traumatic brain injury (TBI) can lead to the disruption of endoplasmic reticulum (ER) homeostasis in neurons and induce ER stress. Transmembrane protein 2 (TMEM2) may regulate stress through p38/ERK signaling pathway, independent classic unfolded response (UPR) pathway. The present study examined expression TMEM2 following TBI a rat model, an aim determine whether mitogen‑activated kinase (MAPK) pathway is controlled by TMEM2/CD44 mitigate secondary injury. For this purpose, 89...

Surgical brain injury (SBI) can disrupt the function of blood‑brain barrier (BBB), leading to edema and neurological dysfunction. Thus, protecting BBB mitigating cerebral are key factors in improving prognosis patients with SBI. The inhibition WNK lysine deficient protein kinase/STE20/SPS1‑related proline/alanine‑rich kinase (SPAK) signaling ameliorates edema, this pathway regulates phosphorylation downstream Na+‑K+‑Cl‑ cotransporter 1 (NKCC1). Therefore, purpose present study was...

BackgroundHistone deacetylase 3 (HDAC3) restores chromatin nucleosomes to a transcriptional repression state, thereby inhibiting gene expression. Studies have found that HDAC3 expression is upregulated in variety of pathological states the central nervous system and related its neurotoxicity. However, role surgical brain injury (SBI) has not been thoroughly explored. Objective: To observe SBI outcome after suppression. Methods: Rat model was used, intraperitoneal injection RGFP966 (HDAC3...

Chronic renal failure (CRF) is associated with hypertriglyceridemia and elevated plasma VLDL IDL concentrations. These events can be due to either increased production or depressed catabolism of triglyceride-rich lipoproteins. Several studies have documented downregulation lipoprotein lipase, hepatic triglyceride the receptor, leading clearance concentration lipoproteins their remnants in CRF. However, effect CRF on biosynthetic pathway has not been explored. Diglycerol acyltransferase...

Pseudopregnancy (PP) in the rat is characterized by maintenance of corpora lutea and twice daily surges prolactin at 1500-2100 h 0100–0900 h. Four days after PP was induced cervical stimulation with a glass rod, rats were cannulated via right carotid artery one day later received an implant ovine (200–250 mg) or albumin (controls) into median eminence hypothalamus. Forty-five 54 implanted showed termination within 4 days. Ova recovered from 19 these whereas all controls remained...

The incidence of traumatic brain injury has been increasing annually. Annexin A7 is a calcium-dependent phospholipid binding protein. It can promote melting the cell membrane. Recent studies have shown that it plays an important role in atherosclerosis, other cardiovascular diseases, and variety tumors. However, few ANXA7 TBI performed. We here observed how changes after discuss whether associated with use antagonist intervention. Experimental results: 1. After TBI, levels were higher than...

Our previous study determined that prominent cerebral vasospasm (CVS) may occur in an vivo model of subarachnoid hemorrhage (SAH) rats. Matrix metalloproteinase 9 (MMP‑9) expression levels basilar arteries were upregulated a similar manner to the development CVS following SAH. To identify changes contractility cerebrovascular smooth muscle cells and MMP‑9 vitro SAH, rat isolated, cultured, then stimulated with hemolysate. Additionally, 2-[(4-phenoxyphenylsulfonyl)methyl]thiirane (SB-3CT),...

Mitochondrial dysfunction caused by mitochondrial DNA (mtDNA) damage and mutation is widely accepted as one of the pathological processes neurodegenerative diseases. As an mtDNA binding protein, transcription factor A (TFAM) maintains integrity through transcription, replication, nucleoid formation, perception, repair. In recent works, overexpression TFAM increased copy count, promoted function, improved neurological The role in diseases has been well explained. However, after surgical brain...