A5065935969- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Digital Holography and Microscopy
- Optical Coherence Tomography Applications
- Advanced Fiber Laser Technologies
- Advanced Electron Microscopy Techniques and Applications
- Photonic Crystal and Fiber Optics
- Mitochondrial Function and Pathology
- Optical Network Technologies
- Photoacoustic and Ultrasonic Imaging
- Advanced Fiber Optic Sensors
- Imbalanced Data Classification Techniques
- Electron Spin Resonance Studies
- Near-Field Optical Microscopy
- Lipid Membrane Structure and Behavior
- Metabolomics and Mass Spectrometry Studies
- ATP Synthase and ATPases Research
- Image Processing Techniques and Applications
- Rough Sets and Fuzzy Logic
- Data Mining Algorithms and Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Optical Imaging and Spectroscopy Techniques
Peking University
2022-2025
University of Electronic Science and Technology of China
2021-2022
National Engineering Research Center of Electromagnetic Radiation Control Materials
2022
Changchun Institute of Technology
2021
Abstract Open-3DSIM is an open-source reconstruction platform for three-dimensional structured illumination microscopy. We demonstrate its superior performance artifact suppression and high-fidelity relative to other algorithms on various specimens over a range of signal-to-noise levels. also offers the capacity extract dipole orientation, paving new avenue interpreting subcellular structures in six dimensions ( xyzθλt ). The available as MATLAB code, Fiji plugin Exe application maximize...
Abstract Fluorescence microscopic imaging is essentially a convolution process distorted by random noise, limiting critical parameters such as speed, duration, and resolution. Though algorithmic compensation has shown great potential to enhance these pivotal aspects, its fidelity remains questioned. Here we develop physics-rooted computational resolution extension denoising method with ensured fidelity. Our approach employs multi-resolution analysis (MRA) framework extract the two main...
Abstract Mitochondrial membrane environmental dynamics are crucial for understanding function, yet high‐resolution observation remains challenging. Here, HBimmCue is introduced as a fluorescent probe localized to inner mitochondrial (IMM) that reports lipid polarity and order changes, which correlate with cellular respiration levels. Using fluorescence lifetime imaging microscopy (FLIM), IMM heterogeneity uncovered across scales, from nanoscale structures within individual mitochondria mouse...
Resolving complex three-dimensional (3D) subcellular dynamics noninvasively in live tissues demands imaging tools that balance spatiotemporal resolution, field-of-view and phototoxicity. Image scanning microscopy (ISM), as an advancement of confocal laser microscopy, provides a 2-fold 3D resolution enhancement. Nevertheless, the relatively low speed has been major obstacle for ISM to be further employed vivo biological tissues. Our proposed solution, multi-confocal image (MC-ISM), aims...
Abstract Despite the grand advances in fluorescence microscopy, photon budget of fluorescent molecules remains fundamental limiting factor for major imaging parameters, such as temporal resolution, duration, contrast, and even spatial resolution. Computational methods can strategically utilize photons against noise, to break abovementioned limits. Here, we propose a multi-resolution analysis (MRA) approach recharacterize extract two main characteristics images: (1) high contrast across edge,...
Abstract In recent years, notable progress has been achieved in both the hardware and algorithms of structured illumination microscopy (SIM). Nevertheless, advancement 3DSIM impeded by challenges arising from speed intricacy polarization modulation. this study, we introduce a high-speed modulation system, leveraging maintaining capabilities digital micro-mirror device (DMD) conjunction with an electro-optic modulator. The DMD-3DSIM system yields 2-fold enhancement lateral (133 nm) axial (300...
Principal component analysis (PCA), a common dimensionality reduction method, is introduced into SIM to identify the frequency vectors and pattern phases of illumination with precise subpixel accuracy, fast speed, noise-robustness, which promising for real-time long-term live-cell imaging.
Under super-resolution imaging conditions, this high hydrophobic far-red probe with a perfluoroalkyl chain revealed the changes clearly in mitochondrial cristae morphology during autophagy induced by starvation or apoptosis.
Abstract A fundamental challenge in fluorescence microscopy is the defocused background caused by scattering light, optical aberration, or limited axial resolution. Severe defocus backgrounds will submerge in-focus information and cause artifacts following processing. Here, we leverage a priori knowledge about dark channels of biological structures dual frequency separation to develop single-frame removal algorithm. It stably improves signal-to-background ratio structural similarity index...
In recent years, notable progress has been achieved in both the hardware and algorithms of structured illumination microscopy (SIM). Nevertheless, advancement three-dimensional (3DSIM) impeded by challenges arising from speed intricacy polarization modulation. We introduce a high-speed modulation 3DSIM system, leveraging polarization-maintaining capabilities digital micromirror device (DMD) conjunction with an electro-optic modulator. The DMD-3DSIM system yields twofold enhancement lateral...
We demonstrate numerically a novel repeater-based three-stage cascaded soliton self-frequency shift (SSFS) structure including germania-core fiber, an Er <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3+</sup> ZBLAN fiber amplifier, and indium fluoride (InF <sub xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> ) or TeO xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> -Bi O -ZnO-Na (TBZN) fiber. Wide wavelength tunability of 2–4.4 μm 2–5.0...
We numerically investigate a Raman soliton source cascaded by fluorotellurite fiber and chalcogenide fiber. By using 2.8 μm laser with pulse width of 137 fs as the pump light, widely tunable solitons spanning from to 8.1μm are observed in nonlinear media 5 cm 10 The red-shift at 8.1 is 132 fs. For first time we propose idea coupling tellurite further enhance SSFS effect mid infrared region (MIR). Our simulated results show possibility generating long wavelength use
We investigate theoretically the mid-infrared (MIR) Raman soliton self-frequency shift (SSFS) process in TeO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> -BaF -Y O xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> (TBY) fiber and AsSe -As S xmlns:xlink="http://www.w3.org/1999/xlink">5</sub> fiber. The numerical analysis of SSFS TBY is performed, revealing impacts pumping wavelength core diameter on effect. A new quantity measuring...
Abstract With optical section and defocus removal effect, three-dimensional structured illumination microscopy (3DSIM) can get a whole sight of intracellular organelle. Here, Open-3DSIM is reported as an open-source reconstruction platform with double improvement on lateral axial resolution. MATLAB code, ImageJ version Exe application are provided for biologists engineers to maximize its user-friendliness prompt further development. Through adaptive parameter estimation spectrum filter...
Abstract Fluorescence microscopic imaging is essentially a convolution process, with noise added during the photon conversion or readout confining speed, duration, and resolution. Aiming at recovering biological information from strong level to improve these key parameters, here we propose multi-resolution analysis (MRA) approach extract two main characteristics of fluorescence images: (1) high contrast across edge, (2) continuity along edge. By regularizing framelet curvelet domain...
In order to improve the accuracy and rapidity of risk early warning active distribution network, this paper adopts idea data mining proposes an improved Apriori algorithm for risks in network. This method takes weather, equipment reasons as research objects, through mathematical analysis, based on seasonal classification, which greatly improves efficiency algorithm, thus improving response speed system. At same time, a failure mode library typical environmental scenarios has been...