- Chromium effects and bioremediation
- Metal Extraction and Bioleaching
- Chemical Reactions and Isotopes
- Wastewater Treatment and Nitrogen Removal
- Mine drainage and remediation techniques
- Radioactive element chemistry and processing
- Microbial Community Ecology and Physiology
- Anaerobic Digestion and Biogas Production
- bioluminescence and chemiluminescence research
- Microbial Fuel Cells and Bioremediation
- Polar Research and Ecology
- Adsorption and biosorption for pollutant removal
- Chemical Analysis and Environmental Impact
- Microbial bioremediation and biosurfactants
- Methane Hydrates and Related Phenomena
- Fungal and yeast genetics research
- Metalloenzymes and iron-sulfur proteins
- Microbial Inactivation Methods
- Polyamine Metabolism and Applications
- Chemical Synthesis and Characterization
- Calcium Carbonate Crystallization and Inhibition
- Enzyme Production and Characterization
- Marine and coastal plant biology
- Food Industry and Aquatic Biology
- Graphene and Nanomaterials Applications
Lviv University
2011-2025
State Institution National Antarctic Scientific Center
2025
This study explores the biodegradation of polyhydroxybutyrate (PHB), polylactide (PLA), and their blends by 11 bacterial species (including Antarctic strains) 6 fungal species. Aeration significantly enhanced PHB degradation mold fungi (Aspergillus oryzae, Penicillium chrysogenum) bacteria (Paenibacillus tundrae, Bacillus mycoides), while Aspergillus awamori was most effective under non-aerated conditions. For PLA, peaked aeration with chrysogenum subtilis. PHB/PLA degraded slower overall,...
The study’s primary purposes were establishing the number of microorganisms that exhibit hydrolytic activity in Antarctic soil and mosses samples, isolation metal-resistant strains bacteria, description their physiological biochemical properties. Samples collected during XXIII Ukrainian Expedition 2019 used. colony-forming units exhibiting proteolytic, amylolytic, cellulase, lipolytic was studied. Pure bacterial cultures isolated using standard microbiological methods. Determination...
As a result of human activity aromatic hydrocarbons enter the environment in large quantities, contaminating it. Dropping insufficiently treated wastewater drains considerably decrease quality water. Quite effective biological methods purification contaminated are usage microorganisms. Prospective microorganisms for sewage treatment sulfate-reducing bacteria. The purpose work was to investigate ability bacteria use xylene and toluene as source carbon under different cultivation conditions....
The review briefly describes the toxic effects of heavy metals towards microorganisms and resistance mechanisms to metals. processes fermentative non-fermentative reduction oxidized forms by sulfate-reducing bacteria factors that have influence these are described. Some sulfate-reducing using as terminal electrons acceptors Data about usage in bioremediation presented. attention is paid psychrophylic processes. Keywords: metals, dissimilatory sulfate reduction, bacteria, bioremediation.
Such compounds as of hexavalent chromium, nitrates and nitrites are one the most distributed pollutants environment. The Cr (VI) found in soil, water territories adjacent to mining enterprises elevated concentrations. chromium has toxic, mutagenic carcinogenic effect on living forms. Some sulfur-reducing bacteria attractable for scientists attention like potential purification agentsto clear wastewater from organic inorganic pollutants. In this way,the purpose our work is study patterns...
The aim of this work was to study the patterns utilization sulfate and nitrate ions by bacteria Desulfomicrobium sp. CrR3 Desulfovibrio desulfuricans Ya-11 under different cultivation conditions. Chromium-resistant sulfate-reducing D. were used. Bacteria grown in Posgate C medium at 30°C 25 ml test tubes anaerobic To ability use various substances as electron acceptors, they incubated potassium phosphate buffer (10 mM, pH 7) with sulfate, nitrite concentrations 1, 5 10 mM. At (1, mM),...
The ability of Desulfovibrio desulfuricans Ya-11 bacteria to use nitrate as terminal electron acceptor was studied. level biomass accumulation in the medium with changed depending on initial NO 3 – concentration. At concentration 5 mM bacterial same sulfate. intermediate product nitrates reduction D . is nitrite, and final one ammonium. High concentrations inhibit process dissimilatory sulfate reduction. Washed cells fully reduced nitrite psychrophyllic sulfate-reducing Desulfobacter sp....
In the presence of sulfate in medium? sulfate-reducing bacteria Desulfomicrobium sp. use it as a final electron acceptor with it's reduction to hydrogen sulfide. Except sulfate, Desulfomicrobium are able nitrate, сhromate, trivalent iron – Fe (III), fumarate and elemental sulfur acceptors process organic compounds oxidation. Chromate nitrate provide growth which does not significantly differ from bacterial medium sulfate. Under these conditions, Cr +6 is reduced +3 , nitrates nitrite...
The usage of sodium sulfate and nitrate in the presence potassium biсhromate medium by sulfate-reducing bacteria Desulfomicrobium sp. CrR3 was studied. It shown that 5 mM nitrate, 0.5 bichromate were optimal conditions for bacterial growth. concentration provides better growth microorganisms than sulfate. An increase concentrations to 15 1 led slower In medium, chromium used first, herewith utilisation inhibited. Influence on reduction depends medium. found hydrogen sulfide formation started...
The aim of the study was to investigate number different groups microorganisms in soil samples from sites feed and nesting activity Larus dominicanus (Galindez Island, maritime Antarctic) characterize physiological biochemical properties isolated microorganisms. Methods. In work standard microbiological research methods were used (cultural, microscopy methods, determination enzymatic activity). Genomic DNA by soft lysis. 16 S rRNA gene amplified using universal primers 27F 1492R....
In this review, the peripheral pathways for decomposition of aromatic compounds by bacteria are considered. Aromatic can be degraded with participation under aerobic and anaerobic conditions. presence oxygen, metabolized genera Pseudomonas , Rhodococcus Nocardia Micrococcus Bacillus Arthrobacter others. Under conditions, a benzene core is carried out sulfate-reducing, nitrate-reducing fermenting bacteria. Degradation complex long-term process, which in natural conditions depends on biotic...
This review summarizes current information on the extent of environmental pollution by xenobiotics aromatic nature, and their toxic effects living organisms. Phenols, monocyclic (benzene, toluene, xylene etc) polycyclic hydrocarbons (naphthalene, anthracene, phenanthrene, biphenуls, pyrene, benzpyrene, pyridine etc.) are most dangerous pollutant compounds. A variety microorganisms capable to destroy compounds is described. The biodegradation pollutants carried out denitrifying,...
Aim. To investigate the cultural, morphological, physiological and biochemical properties of isolated chemoorganotrophic heavy metal resistant isolates bacteria from different samples obtained during Ukrainian Antarctic expedition in 2019 for further selection most to compounds biochemically active substances. Methods. Pure cultures were using tryptone soy agar nutrient agar. Obtained sown on media containing Cu(II) (0.3, 0.1, 1.5, 8, 78 mM), Pb(II) (0.00009, 0.0005, 0.005, 0.05, 0.5 Cr(VI)...
The usage of microorganisms to clean the environment from xenobiotics, in particular chlorine-containing ones, is a promising method detoxifying contaminated environment. Sulfate-reducing bacteria Desulfovibrio desulfuricans Ya-11, isolated Yavoriv Lake, and Desulfotomaculum AR1, Lviv sewage treatment system, are able grow under conditions environmental contamination by aromatic compounds substances. Due their high redox potential, chlorate perchlorate ions can be ideal electron acceptors...
Sulphate-reducing bacteria Desulfomicrobium sp. CrR3 and Desulfotomaculum. are able to use fumarate as electron donor acceptor. When they an acceptor succinate accumulates in the medium. If serves donor, minor amounts of citrate, isocitrate acetate detected except succinate. In case simultaneous introduction fumarate, SO4(2-) Cr2O7(2-), last inhibits usage SO4(2-).
The aim of this work was to investigate some physiological groups microorganisms which are components wastewater microbiocenoses. Microorganisms were grown in Petri dishes containing 20–30 ml agar selective medium and 25 tubes at a temperature +30 ºC. media were: wort for microscopic fungi yeasts, Hutchinson the cellulose-destroying microorganisms, starch-ammonium that can utilize mineral nitrogen forms, Postgate B sulfate-reducing bacteria, Vinogradsky nitrifying Ashby nitrogen-fixing...
Influence of hydrogen sulfide on the cell wall proteins composition Saccharomyces cerevisiae yeasts is investigated. H 2 S considerably destroys outer mannoprotein layer wall. The content most covalently bound proteins, particularly, with molecular weights 17, 24, 32, 37 and 53 kDa, decreased during yeasts' cultivation 10 mM for one day. reducing sugars in medium increased at presence sulfide. At concentration 20 30 none were observed. Among non-covalently S. cerevisiae, increase 110, 64,...
Bacteria performing dissimilatory sulfate reduction were isolated from Yaziv sulfur deposit wells. 12 cultures resistant to the presence of 1 mM Cr (VI) in medium. Addition 5 lead near two-fold biomass and hydrogen sulfide content decrease. At absence medium bacteria use as final electron acceptor. latter decreased due its by sulfide. (ІІI) at concentrations 1–5 doesn't does not have considerable inhibitory effect on sulfate-reducing activity. The ability detoxify usage acceptor sulfide,...
Immobilization of microorganisms is an effective method intensification wastewater purification form chromate-containing compounds. This paper presents a sulfate-reducing bacteria immobilization in agar developed order to determine the effectiveness water from toxic hexavalent chromium Dependence influence different factors on reduction compounds by immobilized cells Desulfomicrobium sp. CrR3 has been studied. Both free and agar-immobilized almost completely reduced Cr(VI) at initial...
Sulfur-reducing bacteria are promising agents for the development of new methods wastewater treatment with removal ions heavy metals and organic compounds. Study effect various environmental factors on growth sulfidogenic activity sulfur-reducing allows one to investigate adaptability these microorganisms stress factors. The paper deals рН, different concentrations elemental sulfur, hydrogen sulfide presence electron acceptors Desulfuromonas sp. YSDS-3. calculation C/S ratio YSDS-3 was made,...
Purification of the aquatic environment from substances organic and inorganic matter is an urgent problem nowadays. Falling into water insufficiently treated effluents significantly impairs quality condition bodies in general. Aromatic compounds, particular toluene xylene, are quite common pollutants environment. In addition to compounds nature, wastewater contains many heavy metal ions. Among methods detoxification environment, biological ones play a leading role, as they cost-effective...
Introduction. Thermophilic sulfate-reducing bacteria attract attention of scientists as the potential agents purification wastewater polluted by sulfur and its compounds, heavy metal ions organic compounds. These oxidize different substrates using metals with variable valency electron acceptors transform them into non-toxic or less toxic forms for living organisms. However, contains high concentrations xenobiotics, particularly, that have negative influence on For this reason, it is...