A5075127074- Immune Cell Function and Interaction
- Pediatric Hepatobiliary Diseases and Treatments
- Diabetes and associated disorders
- CRISPR and Genetic Engineering
- Liver Disease Diagnosis and Treatment
- CAR-T cell therapy research
- Liver Diseases and Immunity
- Pancreatic function and diabetes
Virginia Mason Medical Center
2022-2024
Benaroya Research Institute
2022-2024
Adoptive transfer of regulatory T cells (Tregs) is therapeutic in type 1 diabetes (T1D) mouse models. Tregs that are specific for pancreatic islets more potent than polyclonal preventing disease. However, the frequency antigen-specific natural extremely low, and ex vivo expansion may destabilize Tregs, leading to an effector phenotype. Here, we generated durable, engineered (EngTregs) from primary human CD4+ by combining FOXP3 homology-directed repair editing lentiviral cell receptor (TCR)...
Adoptive regulatory T (Treg) cell therapy is predicted to modulate immune tolerance in autoimmune diseases, including type 1 diabetes (T1D). However, the requirement for antigen (ag) specificity optimally orchestrate tissue-specific, Treg cell-mediated limits effective clinical application. To address this challenge, we present a single-step, combinatorial gene editing strategy utilizing dual-locus, dual-homology-directed repair (HDR) generate and specifically expand ag-specific engineered...