A5008266468- Wnt/β-catenin signaling in development and cancer
- Cancer-related gene regulation
- Neurobiology and Insect Physiology Research
- Cephalopods and Marine Biology
- Kruppel-like factors research
- Developmental Biology and Gene Regulation
- Retinal Development and Disorders
- Cellular Mechanics and Interactions
- Cell Adhesion Molecules Research
- Physiological and biochemical adaptations
- Planarian Biology and Electrostimulation
- Echinoderm biology and ecology
- 3D Printing in Biomedical Research
- Congenital heart defects research
- Photoreceptor and optogenetics research
- Pancreatic function and diabetes
- Pluripotent Stem Cells Research
- Adenosine and Purinergic Signaling
- Microtubule and mitosis dynamics
- Invertebrate Immune Response Mechanisms
- Barrier Structure and Function Studies
- Nuclear materials and radiation effects
- Infectious Diseases and Tuberculosis
- Steroid Chemistry and Biochemistry
- Photochromic and Fluorescence Chemistry
Kitasato University
2009-2024
Yokohama City University
2000-2022
Keio University
2019
Kwansei Gakuin University
2009
Nagoya University
1987-2006
Doheny Eye Institute
1993-1998
University of Southern California
1994-1998
ABSTRACT Cell adhesion and several other properties of a recently identified cadherin-related protein, protocadherin Pcdh2, were characterized. A chimeric Pcdh2 in which the original cytoplasmic domain was replaced with E-cadherin expressed mouse L cells. The protein had molecular mass about 150 kDa localized predominantly at cell periphery, as wild-type Pcdh2. In conventional aggregation assay, transfectants showed activity comparable to that classical cadherins. This Ca2+-dependent...
ABSTRACT Several properties of cadherin-4 and cadherin-5 were char- acterized by using the cDNA transfection approach. The proteins both cadherins had a relative molecular mass about 130 kDa present at cell periphery, especially cell-cell contact sites. These easily digested with trypsin, Ca2+ protected cadherin- 4, but not cadherin-5, from digestion. In immunopre- cipitation, co-precipitated two major 105 95 kDa, respectively. are likely to correspond α- β-catenins. Cadherin-5 only one...
Journal Article Desmocollin-2 alone forms functional desmosomal plaques, with the plaque formation requiring juxtamembrane region and plakophilins Get access Miwako Fujiwara, Fujiwara 1Department of Bioscience, School Science Technology, Kwansei Gakuin University, Sanda-shi, Hyogo-ken 669-1337, Japan; Search for other works by this author on: Oxford Academic PubMed Google Scholar Azusa Nagatomo, Nagatomo Megumi Tsuda, Tsuda Shuichi Obata, Obata 2Department Liberal Arts, Kitasato...
AbstractTo study the diversity of protocadherins, a rat brain cDNA library was screened using for cytoplasmic domain human protocadherin Pcdh2 as probe. The resultant clones contained three different types. One type corresponds to Pcdh2; other two types are distinct from but contain same sequence in their domains and part 3′ flanking sequence. To clarify structure proteins defined by new clones, putative entire coding corresponding one determined. overall is essentially Pcdh2, indicating...
Abstract The newt, a group of urodele amphibians, has outstanding ability to repeatedly regenerate various body parts, even in the terrestrial life-stage. In this animal, when limb is amputated, cell mass named blastema appears on stump and eventually gives rise new functional limb. Erythrocytes (red blood cells) most non-mammalian vertebrates, including preserve their nucleus throughout life-span, although physiological roles such nucleated erythrocytes, other than oxygen delivery, are not...
When cybrids with a point mutation, which locates in the tRNALeu(UUR) gene of mtDNA and causes mitochondrial encephalomyopathy (MELAS syndrome), were exposed to high concentration oxygen (95%), peroxide production markedly increased by 6 h exposure, whereas was similar among under normal oxygen. The exposure enhanced particularly proportions mutant low respiratory capacities. appearance apoptotic cells impaired function due mutation. An antioxidant NAC successfully suppressed both apoptosis....
The accumulation of classical cadherins is essential for their function, but the mechanism poorly understood. Hence, we investigated E- and N-cadherin formation cell junctions in epithelial cells. Immunostaining revealed a scattered dot-like throughout lateral membrane MDCK II other Mutant E-cadherin lacking β-catenin binding site accumulated granularly at cell–cell contact sites showed weak aggregation activity cadherin-deficient cells, MIA PaCa2 p120-catenin exhibited punctate strong...
The role of p120-catenin in the function classical cadherins is still enigmatic despite various studies. To elucidate its role, we examined effect on N-cadherin-mediated localization junctional proteins epithelial cells this study. Cadherin-deficient MIA PaCa-2 did not show linear tight junction ZO-1 and occludin. When N-cadherin was expressed these cells, however, resultant transfectant revealed strong cell adhesion activity ZO-1, occludin, lateral membrane. p120-catenin-binding site...
It is well known that extracellular ATP (ATP o ) elevates the intracellular Ca 2+ concentration ([Ca ] i by inducing influx or mobilizing from internal stores via activation of purinoceptors in plasma membrane. This study shows also activates membrane pumps (PMCPs) to bring elevated [Ca back resting level human embryonic kidney‐293 (HEK‐293) cells. The duration ‐induced transients was significantly increased PMCP blockers, La 3+ orthovanadate. In contrast, replacement Na + with NMDG , a...
Daptomycin (DAP) is one of the most potent antibiotics used for treatment methicillin-resistant Staphylococcus aureus (MRSA) infections. Due to an increase in its administration combating MRSA infections, DAP non-susceptible (DAP-NS) strains have recently been reported clinical settings. The presence single nucleotide polymorphisms (SNPs) multiple peptide resistance factor ( mprF ) gene frequently cause evolution DAP-NS strains; however, there are some variations SNPs that could lead DAP-NS....
During vertebrate cardiac development, the heart tube formed by fusion of right and left presumptive mesoderms (PCMs) undergoes looping toward right, resulting in an asymmetrical heart. Here, we examined PCMs with regard to heart-tube using right- left-half newt embryos (Cynops pyrrhogaster ). In half embryos, rightward (normal) loop was from PCM, irrespective timing its separation, while leftward (reversed) separated stage 18. addition, direction inverted right-half bisected after Incision...
Gene editing methods were applied to the study of E-cadherin function in epithelial cells. The gene DLD-1 cells was ablated using TALEN. resultant showed round fibroblast-like morphology and had almost no Ca2+-dependent cell aggregation activity. re-expression knockout restored strong cell-cell adhesion activity, indicating that retained ability support cadherin function. partial localization desmoplakin ZO-1 at intercellular contact sites. transfectants expressing mutant lacking cytoplasmic...
This study investigated the development of Ca 2+ signaling mechanisms and their role in initiating morphogenetic cell movement presumptive ectoderm Japanese newt ( Cynops pyrrhogaster ) during gastrulation. Histochemical staining using fluorescently labeled ryanodine dihydropyridine probes revealed that receptor (L‐type channels) appeared stage 12b embryos, while receptors were expressed both 11 embryos. Transmission electron microscopy embryos showed abundant peripheral couplings, which are...
This study investigated the three-dimensional (3D) cellular interactions and tunneling nanotubes (TNTs) during fetal mouse skin regeneration on embryonic days 13 (E13) 15 (E15). We aimed to understand spatial relationships among cell types involved in assess potential role of TNTs. Full-thickness incisions were performed E13 E15 embryos. Wound sites collected, embedded epoxy resin, processed for 3D reconstruction (1 μm thickness sections), subjected whole-mount immunostaining. conducted...
The G protein messages in Octopus vulgaris photoreceptor were isolated and characterized with molecular biological techniques. Four classes of α subunit cDNA from an octopus eye library: OvGαi, OvGαo, OvGαq OvGαs. Northern blot analysis tissues showed abundant expression the eye, specific OvGαo neural tissues. OvGαi was expressed all studied. In situ hybridization revealed that message almost cells. Based on these results, a possible phototransduction pathway visual cells is discussed.