A5008151683- Tuberculosis Research and Epidemiology
- Bacteriophages and microbial interactions
- Ionic liquids properties and applications
- RNA and protein synthesis mechanisms
- Mycobacterium research and diagnosis
- Bacterial Genetics and Biotechnology
- Electrochemical Analysis and Applications
- Evolution and Genetic Dynamics
- Inorganic and Organometallic Chemistry
- Chemical and Physical Properties in Aqueous Solutions
- Cancer therapeutics and mechanisms
- Multicomponent Synthesis of Heterocycles
University of Michigan
2013-2017
State University of New York
2013
SUNY Brockport
2012
Since 1967, Rifampin (RMP, a Rifamycin) has been used as first line antibiotic treatment for tuberculosis (TB), and it remains the cornerstone of current short-term TB treatment. Increased occurrence Rifamycin-resistant (RIFR ) TB, ∼41% which results from RpoB S531L mutation in RNA polymerase (RNAP), become growing problem worldwide. In this study, we determined X-ray crystal structures Escherichia coli RNAPs containing most clinically important two other frequently observed RIFR mutants,...
Rifampin, a semisynthetic rifamycin, is the cornerstone of current tuberculosis treatment. Among many rifamycins, benzoxazinorifamycins have great potential for TB treatment due to their superior affinity wild-type and rifampin-resistant Mycobacterium RNA polymerases reduced hepatic Cyp450 induction activity. In this study, we determined crystal structures Escherichia coli polymerase complexes with two benzoxazinorifamycins. The ansa-naphthalene moieties bind in deep pocket β subunit,...
The structural and dynamic behavior of the ionic liquid 1-hexyl-3-methylimidazolium bis(trifluoromethylsulfonyl)amide ([C(6)mim][NTf(2)]) in chloroform has been studied by experimental measurements (1)H (19)F self-diffusion coefficients, viscosity, excess molar volume concentration range 0.001-1.0 mol·kg(-1) temperatures ranging from 15 to 45 °C. Within measurement uncertainty, coefficients are identical at same conditions temperature, indicating that even lowest measured concentrations...
Rifampin has been a cornerstone of tuberculosis (TB) treatment since its introduction. The rise multidrug-resistant and extensively drug-resistant TB makes the development novel therapeutics effective against these strains an urgent need. Site-specific mutations in target enzyme rifampin, RNA polymerase (RNAP) comprises majority (~97%) rifamycin-resistant (RifR) Mycobacterium (MTB). To identify inhibitors bacterial RNAP, vitro plasmid-based transcription assay that uses malachite green (MG)...
Rifampin has been a cornerstone of tuberculosis (TB) treatment since its introduction. The rise multidrug-resistant and extensively drug-resistant TB makes the development novel therapeutics effective against these strains an urgent need. Site-specific mutations in target enzyme rifampin, RNA polymerase (RNAP) comprises majority (~97%) rifamycin-resistant (RifR) Mycobacterium (MTB). To identify inhibitors bacterial RNAP, vitro plasmid-based transcription assay that uses malachite green (MG)...