A5085950028- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Polysaccharides and Plant Cell Walls
- DNA and Nucleic Acid Chemistry
- DNA Repair Mechanisms
- Carbohydrate Chemistry and Synthesis
- Spectroscopy and Quantum Chemical Studies
- Asthma and respiratory diseases
- Wheat and Barley Genetics and Pathology
- Photochemistry and Electron Transfer Studies
- Crystallography and molecular interactions
- Microbial Metabolites in Food Biotechnology
- Polish-Jewish Holocaust Memory Studies
- Pharmacological Effects of Natural Compounds
- Synthetic Organic Chemistry Methods
- Molecular Sensors and Ion Detection
- DNA and Biological Computing
- Education and Cultural Studies
- Escherichia coli research studies
- Plant Growth Enhancement Techniques
- Cancer Genomics and Diagnostics
- Sugarcane Cultivation and Processing
- Fetal and Pediatric Neurological Disorders
- Advanced Proteomics Techniques and Applications
- Protein Tyrosine Phosphatases
University of Guelph
2025
University of Gdańsk
2020-2023
Institute of Technology and Life Sciences
2022
La Roche College
2009-2015
Górnośląskie Centrum Medyczne
2014
Wojewódzki Szpital Zespolony
2013
The King's College
2010
Vanderbilt University
2000-2005
Oregon Health & Science University
2005
The University of Texas Medical Branch at Galveston
2002
Complex mixtures of DNA encoded small molecules may be readily interrogated via high-throughput sequencing. These libraries (DELs) are commonly used to discover that interact with pharmaceutically relevant proteins. The chemical diversity displayed by the library is key successful discovery potent, novel, and drug-like matter. molecule moieties DELs generally synthesized though a multistep process, each step accomplished while it simultaneously attached an encoding oligomer. Hence, often...
To initiate studies designed to identify the mutagenic spectrum associated with butadiene diepoxide-induced N2-N2 guanine intrastrand cross-links, site specifically adducted oligodeoxynucleotides were synthesized in which bases centrally located within context of human ras 12 codon. The two stereospecifically modified DNAs and corresponding unmodified DNA ligated into a single-stranded M13mp7L2 vector transfected intoEscherichia coli. Both stereoisomeric forms (R,R S,S) cross-links resulted...
Pectobacterium parmentieri is a pectinolytic plant pathogenic bacterium causing high economic losses of cultivated plants. The highly devastating potential this phytopathogen results from the efficient production cell wall-degrading enzymes, i.e., pectinases, cellulases and proteases, in addition to impact accessory virulence factors such as motility, siderophores, biofilm lipopolysaccharide (LPS). LPS belongs pathogen-associated molecular patterns (PAMPs) plays an important role...
Pectobacterium brasiliense is a widespread plant pathogenic bacterium classified to the Pectobacteriaceae family, which causes significant economic losses because of developed soft rot and blackleg symptoms on potatoes wide spectrum crops, vegetables, ornamentals. One key virulence factors lipopolysaccharide due its involvement in efficient colonisation tissues overcoming host defence mechanisms. Thus, we structurally characterised O-polysaccharide from LPS P. strain IFB5527 (HAFL05) using...
Soft rot and blackleg diseases, caused by pectinolytic bacteria from the numerous species of Dickeya Pectobacterium, pose a serious threat to world potato production. Besides, infections triggered these lead huge economic losses in cultivation other crops, vegetables, ornamentals. Strains belonging genus Pectobacterium tend be isolated various environments such as rotten or asymptomatic plants, weeds, soil water. The main virulence factors phytopathogenic involve plant cell wall degrading...
The inhibition of LTB(4) binding to and activation G-protein-coupled receptors BLT1 BLT2 is the premise a treatment for several inflammatory diseases. In lead optimization effort starting with leukotriene B(4) (LTB(4)) receptor antagonist (2), members series 3,5-diarylphenyl ethers were found be highly potent inhibitors receptors, varying levels selectivity depending on substitution. addition, compounds 33 38 from this have good in vitro ADME properties, oral bioavailability, efficacy after...
A general protocol for preparation of oligonucleotides containing intrastrand cross-links between the exocyclic amino groups adjacent deoxyguanosines has been developed. series 2, 3, and 4 methylene was incorporated site-specifically into an 11-mer (5'-GGCAGGTGGTG-3', cross-linked positions are underlined) via a reaction oligonucleotide 2-fluoro-O6-trimethylsilylethyl deoxyinosines appropriate diamine (ethylenediamine, 1,3-diaminopropane, 1,4-diaminobutane). These...
Pectinolytic bacteria from the genus Pectobacterium cause high economic losses in various crops, vegetables, and ornamentals including potato. Thus far, these strains have been isolated distinct environments such as rotten or asymptomatic plants, soil, waterways. The prevalence of soft rot Pectobacteriaceae different depths Pomeranian lakes was performed by a qualified scuba diver over 2 years monitoring. It allowed for isolation broad characterization strain newly established species...
Abstract The National Toxicology Program has recently classified 1,3‐butadiene (BD) as a human carcinogen. BD is metabolized to the intermediates 1,2‐epoxy‐3‐butene (EB), 1,2:3,4‐diepoxybutane (DEB), and 1,2‐dihydroxy‐3,4‐epoxybutane. All three metabolites have been implicated in producing specific types of DNA damage genotoxic agents mice, rat, cells. This study focused on EB‐induced N1 deoxyinosine lesions that are formed by deamination deoxyadenosine following reaction epoxide at N 1...
A straightforward and cost-effective synthesis of 5-aryl-3-alkylsulfonyl-phenols by a sequential scaffold derivatization strategy has been developed. The procedure is suitable for parallel small libraries around the biphenyl privileged core having an unusual 1,3,5-substitution pattern. exemplified pilot library 30 compounds.
Hydroxyethyl adducts arising by the reactions of simple epoxides at N1 position adenine nucleosides can deaminate to give inosine analogues which, if formed in DNA, are suspected being highly mutagenic. A method has been developed for synthesis oligonucleotides containing N1-adducted 2'-deoxyinosines. The 2'-deoxyinosine adduct 3,4-epoxy-1-butene was prepared from (±)-4-acetoxy-3-bromo-1-butene and tetraisopropyldisiloxanediyl-protected with base. derivative then incorporated into...
This paper investigates one of the “posthumanist” propositions redefining notion community. It argues that in order to expand community as regards nonhuman animals (Haraway) and technology, things (Latour) it is necessary go beyond dichotomy means ends, instrumental communicative action.
The synthesis of methyl 2-amino-6-methoxynicotinate, a valuable building block for the preparation fused 2-pyridones, is reported. optimized includes sequential microwave-induced regioselective 6-methoxylation, esterification, followed by reaction with p-methoxybenzylamine, and final deprotection under flow hydrogenation conditions. Two key steps in reported are methoxylation microfluidic that afford improved regioselectivity purity profile products.
The solution structure of the N1-[1-hydroxy-3-buten-2(R)-yl]-2'-deoxyinosine adduct arising from alkylation adenine N1 by butadiene epoxide (BDO), followed deamination to deoxyinosine, was determined in oligodeoxynucleotide 5'-d(CGGACXAGAAG)-3'·5'-d(CTTCTTGTCCG)-3'. This contained BDO at second position codon 61 human N-ras protooncogene (underlined) and named ras61 R-N1-BDO-(61,2) adduct. 1H NMR revealed a weak C5 H1' X6 H8 nuclear Overhauser effects (NOE), an intense NOE. Simultaneously,...