A5079444362- Immune Cell Function and Interaction
- Cancer Immunotherapy and Biomarkers
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- CAR-T cell therapy research
- Immune cells in cancer
- HIV Research and Treatment
- Immune responses and vaccinations
Stanford University
2024
University of Pennsylvania
2022
UPMC Hillman Cancer Center
2022
Cancer Research Institute
2022
New York University
2014
APOBEC3A (A3A), one of the seven-member APOBEC3 family cytidine deaminases, lacks strong antiviral activity against lentiviruses but is a potent inhibitor adeno-associated virus and endogenous retroelements. In this report, we characterize biochemical properties mammalian cell-produced catalytically active E. coli-produced A3A. The enzyme binds to single-stranded DNA with Kd 150 nM forms dimeric monomeric fractions. A3A, unlike APOBEC3G (A3G), deaminates substrates nonprocessively. Using...
SUMMARY Availability of effective anti-tumor T cells is limited by cancer immunoediting, which depletes neoantigens, and central tolerance, eliminates developing with high-affinity cell receptors (TCRs) against tumor self-antigens. Remaining tumor-reactive are often exhausted after immune checkpoint blockade (ICB). Whether endogenous high- affinity TCRs self-antigens can be generated to circumvent exhaustion reject neoantigen-poor tumors unclear. We show that transiently interrupting...