A5055236875- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Genetic factors in colorectal cancer
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Colorectal Cancer Treatments and Studies
- Molecular Biology Techniques and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Colorectal Cancer Screening and Detection
- Ferroptosis and cancer prognosis
- Microscopic Colitis
- Cancer, Lipids, and Metabolism
- Viral-associated cancers and disorders
- Advanced biosensing and bioanalysis techniques
- Parvovirus B19 Infection Studies
- PI3K/AKT/mTOR signaling in cancer
- Intellectual Property Rights and Media
- Cancer Diagnosis and Treatment
- Bone and Dental Protein Studies
- Vitamin D Research Studies
- Pancreatic and Hepatic Oncology Research
- Bone health and osteoporosis research
Ferdowsi University of Mashhad
2017-2025
Mashhad University of Medical Sciences
2012-2025
Imam Reza Hospital
2025
Czech Academy of Sciences, Institute of Biotechnology
2024
Efficient long-term cultivation of chicken primordial germ cells (cPGCs) is essential for various avian research and biotechnology applications. Our study aimed to address the challenge inconsistent culture success by investigating strain-specific variations optimizing conditions using two distinct media: Ovotransferrin-enriched medium (OTM) serum-supplemented (CSM). We demonstrated that each strain has unique nutritional requirements, with Hubbard cPGCs thriving in OTM Bovans favoring CSM....
Hereditary cancer syndromes account for 6-10% of all colorectal (CRC) cases and 20% early-onset CRC. Identifying novel pathogenic germline variants can impact genetic testing, counseling, surveillance. This study aimed to determine the prevalence associated with hereditary CRC in Iranian population. Whole exome sequencing (WES) was conducted on DNA from 101 patients Colorectal Cancer Registry (IHCCR). The cohort included 63 high-risk Lynch Syndrome (LS) 38 polyposis patients. Germline...
Abstract The cultivation and expansion of chicken primordial germ cells (cPGCs) are critical importance for both biotechnological applications the management poultry genetic biodiversity. feeder-free culture system has become most popular approach cPGCs. However, despite some success in cPGCs, reproducibility conditions across different laboratories remains a challenge. This study aimed to compare two defined enriched media growth cPGCs originating from Hubbard JA57 broiler. To this end,...
One of the most prominent questions in field transgenesis is 'Where genome to integrate a transgene?'. Escape from epigenetic silencing and promoter shutdown transgene needs reliable genomic safe harbor (GSH) loci. Advances engineering technologies combined with multi-omics bioinformatics data have enabled rational evaluation GSH loci host genome. Currently, no validated been evaluated chicken genome.Here, we analyzed experimentally examined two cells. To this end, putative including...
Abstract Background Hormone-dependent promoters are very efficient in transgene expression. Plasmid-based reporter assays have identified regulatory sequences of the Ovalbumin promoter that involved response to estrogen and shown deletion steroid-dependent element (SDRE) negative (NRE) leads a steroid-independent expression reporter. However, functional roles these elements within native genomic context not been evaluated. Results In this study, we show effects NRE on gene can be...
Colorectal cancer (CRC) is a common associated with poor outcomes, underscoring need for the identification of novel prognostic and therapeutic targets to improve outcomes. This study aimed identify genetic variants differentially expressed genes (DEGs) using genome-wide DNA RNA sequencing followed by validation in large cohort patients CRC.
The ability and efficiency of targeted nucleases to perform sequence replacements or insertions into the genome are limited. This limited for can be explained by dependency on DNA repair pathways, possibility cellular toxicity, unwanted activation proto-oncogenes. piggyBac (PB) transposase uses a very efficient enzymatic mechanism integrate fragments in random manner. In this study, we fused an RNA-guided catalytically inactive Cas9 (dCas9) PB used dual sgRNAs localize molecule specific...
Dentin matrix protein 1 (DMP1) is highly expressed in mineralized tooth and bone, playing a critical role mineralization phosphate metabolism. One important for the expression of DMP1 nucleus preosteoblasts up-regulation osteoblast-specific genes such as osteocalcin alkaline phosphatase1 . The present study aimed to investigate potential application human promoter an indicator marker osteoblastic differentiation.In study, we developed promoter-DsRed-GFP knock-in mesenchymal stem cell (MSCs)...
Introduction: Gastrointestinal cancers are among the most common worldwide. Since some viral infections carcinogenic, they may lead to gastrointestinal tract (GIT) neoplasms. This study aimed at investigating relationship of GIT with John Cunningham Virus (JCV), Herpes Simplex virus (HSV), Epstein-Barr (EBV), Cytomegalovirus (CMV), and Human Papillomavirus (HPV).
Colorectal cancer (CRC) imposes a significant healthcare burden globally, prompting the quest for innovative biomarkers to enhance diagnostic and therapeutic strategies. This study investigates G-protein signaling modulator (GPSM) family across several cancers presents comprehensive pan-cancer analysis of GPSM2 gene gastrointestinal (GI) cancers. Leveraging bioinformatics methodologies, we investigated expression patterns, protein interactions, functional enrichments, prognostic...
Abstract Background The cultivation and expansion of chicken primordial germ cells (cPGCs) is critical importance for both biotechnological applications the management poultry genetic biodiversity. feeder-free culture system has become most popular approach cells. However, despite some success in PGCs, reproducibility conditions across different laboratories remains a challenge. This study aimed to compare two defined enriched media growth cPGCs originating from Hubbard JA57 broiler. Methods...
Avian transgenesis has served as a suitable approach to generate bioreactors for the manufacturing of recombinant proteins. Production in chicken cells comes with significant advantages over other systems including providing human-like glycosylation on target In this regard, oviduct-specific ovalbumin promoter been one ideal candidates drive expression transgenes. Previous plasmid-based studies regulatory sequences have led researchers exploit elements out their native genomic context (ex...
To achieve a reliable and persistent expression over time, the transgene should be precisely integrated into genome safe harbor (GSH) loci. Little attention has been paid to find loci of chicken (Gallus gallus domesticus) genome. Identification characterization GSH that allow knocked-in genes could major area interest within field transgenic technology central development bioreactor livestock. Randomly transgenes might encountered position effects epigenetic silencing, so unstable phenotypes...