A5010991875- Molecular spectroscopy and chirality
- Spectroscopy and Quantum Chemical Studies
- Protein Structure and Dynamics
- DNA and Nucleic Acid Chemistry
- Advanced NMR Techniques and Applications
- Glycosylation and Glycoproteins Research
- Antimicrobial Peptides and Activities
- Axial and Atropisomeric Chirality Synthesis
- Protein purification and stability
- Microbial Natural Products and Biosynthesis
- Microbial Metabolism and Applications
- Neuroendocrine Tumor Research Advances
- Cancer Research and Treatments
- Chemical Synthesis and Analysis
- Molecular Spectroscopy and Structure
- Lanthanide and Transition Metal Complexes
- Photoreceptor and optogenetics research
- Radioactive element chemistry and processing
- Marine Sponges and Natural Products
- Photochemistry and Electron Transfer Studies
UiT The Arctic University of Norway
2022-2025
Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry
2019-2024
Czech Academy of Sciences
2024
Centre for Arctic Gas Hydrate, Environment and Climate
2024
Charles University
2019-2020
A marine-derived Pleosporales fungus, Uzbekistanica storfjordensis, was isolated from driftwood and described as a new species. The fungus cultivated in liquid media molecular networking-driven approach used to identify potential secondary metabolites. targeted compounds were using preparative HPLC-MS, through extensive spectroscopic analysis, eight ophiobolin-type sesterterpenes, bipolarolides H–O (1–8), identified. absolute configurations of the determined by ECD assessment. Bipolarolide L...
Vibrational circular dichroism (VCD) spectroscopy has been widely used to study (bio)molecules in solution. However, its solid-state applications have restricted due experimental limitations and artifacts. Having overcome some of them, the first VCD nucleoside crystals is now presented. A two-orders-of-magnitude enhancement signal was observed high molecular order resulting supramolecular chirality. This allowed obtain high-quality spectra within minutes using minute amounts samples. The...
The physical stability of peptide-based drugs is great interest to the pharmaceutical industry. Glucagon-like peptide 1 (GLP-1) a 31-amino acid hormone, analogs which are frequently used in treatment type 2 diabetes. We investigated GLP-1 and its C-terminal amide derivative, GLP-1-Am, both aggregate into amyloid fibrils. While off-pathway oligomers have been proposed explain unusual aggregation kinetics observed previously for under specific conditions, these not studied any detail. Such...
The absolute configuration of cyclic peptides with two chiral centers can be unambiguously assigned VCD. This is however not possible three or four centers. Hence, other techniques are needed to supplement
Protein fibrils are involved in a number of biological processes. Because their structure is very complex and not completely understood, different spectroscopic methods used to monitor aspects fibril structure. We have explored circularly polarized luminescence (CPL) induced lanthanide compounds indicate growth discriminate among types. For hen egg-white lysozyme polyglutamic acid-specific CPL, spectral patterns were obtained could be correlated with vibrational circular dichroism (VCD)...
Amyloidal protein fibrils occur in many biological events, but their formation and structural variability are understood rather poorly. We systematically explore fibril polymorphism for polyglutamic acid (PGA), insulin hen egg white lysozyme. The were grown the presence of "seeds", that is same or different protein. seeds concentrations higher than about 5 % total amount fully determined structure final fibrils. Fibril was monitored by vibrational circular dichroism (VCD) spectroscopy other...
Cationic, antimicrobial peptides (AMPs) are abundantly present in nature as host-defensive peptides, forming the backbone of natural defence many organisms. Their primary interaction with bacterial membranes is thought to be a binding outer layer, followed by disruption lipid membrane. Investigating mode-of-action AMPs an ever-growing field, where plethora different biophysical experiments used investigate this. Here, we explore experimental limitation NMR spectroscopy migrate towards...
Invited for the cover of this issue are Valery Andrushchenko, Monika Krupová, and co-workers at Institute Organic Chemistry Biochemistry (IOCB Prague) Czech Academy Sciences. The image depicts a "crystal city" illuminated by "chiral suns" shining left- right-circularly polarized light (L-CPL R-CPL), which reveals differences in structure chiral crystalline "skyscrapers". Designed Tomáš Belloň @ IOCB Prague. Read full text article 10.1002/chem.202201922.