A5076292430- Chemical Synthesis and Analysis
- Neurobiology and Insect Physiology Research
- Nicotinic Acetylcholine Receptors Study
- Ion channel regulation and function
- Immunotherapy and Immune Responses
- Force Microscopy Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Receptor Mechanisms and Signaling
- Protein Structure and Dynamics
- Glycosylation and Glycoproteins Research
- RNA and protein synthesis mechanisms
- Biochemical and Structural Characterization
- interferon and immune responses
- Microbial Metabolic Engineering and Bioproduction
- Supramolecular Self-Assembly in Materials
- Inflammasome and immune disorders
- Advanced Electron Microscopy Techniques and Applications
- Enzyme Catalysis and Immobilization
- Bacteriophages and microbial interactions
- Peptidase Inhibition and Analysis
- Venomous Animal Envenomation and Studies
- Molecular spectroscopy and chirality
- Enzyme Structure and Function
- Lipid metabolism and biosynthesis
University of California, Irvine
2022-2025
University College London
2017-2024
UC Irvine Health
2024
Transnational Press London
2019
Eastern Regional Research Center
1991
Agricultural Research Service
1991
Spider venom toxins, such as Protoxin-II (ProTx-II), have recently received much attention selective Nav1.7 channel blockers, with potential to be developed leads for the treatment of chronic nocioceptive pain. ProTx-II is a 30-amino acid peptide three disulfide bonds that has been reported adopt well-defined inhibitory cystine knot (ICK) scaffold structure. Potential drawbacks peptides include poor pharmacodynamics and scrambling in vivo. In order address these issues, present study we...
Abstract Lipases from Geotrichum candidum NRRL Y‐553 are of interest because their unique specificity for cis ‐9‐unsaturated fatty acids relative to both stearic and palmitic acids. The lipases were partially purified by chromatography on Octyl Sepharose, AG MP‐1 macroporous anion exchanger, chromatofocusing resin. preparation was found contain multiple, glycosylated varying slightly in pI (pI 4.88, 4.78, 4.65, 4.57 4.52) as judged activity silver staining. molecular mass determined sodium...
A framework is presented to derive the conformational sampling of isoleucine side chains from nuclear magnetic resonance <sup>13</sup>C chemical shifts.
The δ-conotoxins, a class of peptides produced in the venom cone snails, are interest due to their ability inhibit inactivation voltage-gated sodium channels causing paralysis and other neurological responses, but difficulties isolation synthesis have made structural characterization challenging. Taking advantage recent breakthroughs computational algorithms for structure prediction that modeling especially useful when experimental data is sparse, this work uses both deep-learning-based...
Peptide toxins that adopt the inhibitory cystine knot (ICK) scaffold have very stable three-dimensional structures as a result of conformational constraints imposed by configuration three disulfide bonds are hallmark this fold. Understanding oxidative folding pathways these complex peptides, many which important therapeutic leads, is in order to devise reliable synthetic routes correctly folded, biologically active peptides. Previous research on ICK peptide ProTx-II has shown absence an...
Symmetric protein assemblies play important roles in nature which makes them an attractive target for engineering. De novo symmetric complexes can be created through computational design to tailor their properties from first principles, and recently several nanocages have been by bringing together components hydrophobic interactions. Accurate experimental structures of newly-developed proteins are essential validate design, improve assembly stability, downstream applications. We describe the...
The delta-conotoxins, a class of peptide produced in the venom cone snails, are interest due to their ability inhibit inactivation voltage-gated sodium channels causing paralysis and other neurological responses, but difficulties isolation synthesis have made structural characterization challenging. Taking advantage recent breakthroughs computational algorithms for structure prediction that modeling especially useful when experimental data is sparse, this work uses both deep-learning based...
ABSTRACT Intrinsically disordered proteins (IDPs) often contain proline residues, which undergo cis/trans isomerisation. While molecular dynamics (MD) simulations have the potential to fully characterise cis and trans sub-ensembles, they are limited by slow timescales of isomerisation force field inaccuracies. Nuclear magnetic resonance (NMR) spectroscopy can report on ensemble-averaged observables for both states, but a full atomistic characterisation these sub-ensembles is challenging....
Abstract Voltage-gated sodium channels (Na V s) selectively permit diffusion of ions across the cell membrane and, in excitable cells, are responsible for propagating action potentials. One nine human Na isoforms, 1.8, is a promising target analgesics, and selective inhibitors interest as therapeutics. such inhibitor, gating-modifier peptide Protoxin-I derived from tarantula venom, blocks channel opening by shifting activation voltage threshold to more depolarised potentials, but structural...