A5076693498- Photoreceptor and optogenetics research
- Receptor Mechanisms and Signaling
- Spectroscopy and Quantum Chemical Studies
- Photosynthetic Processes and Mechanisms
- Mitochondrial Function and Pathology
- Retinal Development and Disorders
- Chalcogenide Semiconductor Thin Films
- Molecular spectroscopy and chirality
- Chemical and Physical Properties in Aqueous Solutions
- Mass Spectrometry Techniques and Applications
- Photorefractive and Nonlinear Optics
- Neural dynamics and brain function
- Genomics and Phylogenetic Studies
- ATP Synthase and ATPases Research
- Neuroscience and Neuropharmacology Research
- Photochromic and Fluorescence Chemistry
- Advanced NMR Techniques and Applications
- Solid-state spectroscopy and crystallography
- Electrochemical Analysis and Applications
Birkbeck, University of London
2022-2024
Institute of Structural and Molecular Biology
2022-2024
Paul Scherrer Institute
2018-2023
ETH Zurich
2018
Abstract Mitochondrial energy conversion requires an intricate architecture of the inner mitochondrial membrane 1 . Here we show that a supercomplex containing all four respiratory chain components contributes to curvature induction in ciliates. We report cryo-electron microscopy and cryo-tomography structures comprises 150 different proteins 311 bound lipids, forming stable 5.8-MDa assembly. Owing subunit acquisition extension, complex I associates with IV dimer, generating wedge-shaped gap...
Abstract Vision is initiated by the rhodopsin family of light-sensitive G protein-coupled receptors (GPCRs) 1 . A photon absorbed 11- cis retinal chromophore rhodopsin, which isomerizes within 200 femtoseconds to all- trans conformation 2 , thereby initiating cellular signal transduction processes that ultimately lead vision. However, intramolecular mechanism photoactivated induces activation events inside remains experimentally unclear. Here we use ultrafast time-resolved crystallography at...
Selective coupling of G protein (heterotrimeric guanine nucleotide-binding protein)-coupled receptors (GPCRs) to specific Gα-protein subtypes is critical transform extracellular signals, carried by natural ligands and clinical drugs, into cellular responses. At the center this transduction event lies formation a signaling complex between receptor protein. We report crystal structure light-sensitive GPCR rhodopsin bound an engineered mini-Go The conformation identical all previous structures...
Respiratory complexes have co-evolved into supercomplexes in different clades to sustain energy production at the basis of eukaryotic life. In this study, using cryogenic electron microscopy, we determined 2.1 Å resolution structure a 104-subunit II2-III2-IV2 supercomplex from parasite Perkinsus marinus, related Apicomplexa, capable complete transport succinate molecular oxygen. A feature is association two copies complex II via apicomplexan subunit SDHG that interacts with both III and IV...
Mitochondrial energy conversion requires an intricate architecture of the inner mitochondrial membrane 1 . Here we show that in ciliates, curvature is provided by a supercomplex containing all four respiratory chain components. We report cryo-electron microscopy and cryo-tomography structures comprises 150 different proteins 311 bound lipids, forming stable 5.8-megadalton assembly. Due to subunit acquisition extension, complex I associates with IV dimer, generating wedge-shaped gap serves as...
Abstract Vision is initiated by the rhodopsin family of light-sensitive G protein-coupled receptors (GPCRs). A photon absorbed 11- cis retinal chromophore which isomerises within 200 femtoseconds to all- trans conformation, thereby initiating cellular signal transduction processes that ultimately lead vision. However, intramolecular mechanism photoactivated induces activation events inside remains elusive. In this work, we use ultrafast time-resolved crystallography at room temperature...