To determine the ability of southern green stink bug (SGSB) (Nezara viridula L.) to transmit Pantoea agglomerans into cotton (Gossypium hirsutum) bolls.An SGSB laboratory colony was kept on fresh beans. A P. variant resistant rifampicin (Rif) (strain Sc 1-R) used as opportunistic pathogen. Adult insects were individually provided beans that sterilized and then soaked in either sterile water or a suspension strain 1-R. Insects caged with an unopened greenhouse-grown boll. After 2 days, live...

ABSTRACT Burkholderia cenocepacia strain K56-2, a representative of the cepacia complex, is part epidemic and clinically problematic ET12 lineage. The produced plant tissue watersoaking (ptw) on onion tissue, which disease-associated trait. Using plasposon mutagenesis, mutants in ptw phenotype were generated. translated sequence disrupted gene ( ptwD4 ) from ptw-negative mutant showed homology to VirD4-like proteins. Analysis region proximal transfer homolog identified cluster located 92-kb...

To investigate the aetiology of seed and boll rot cotton grown in South Carolina (SC).Bacteria were isolated from diseased locules bolls collected a field SC, USA tested for ability to cause comparable disease symptoms greenhouse fruit. Spontaneously generated rifampicin-resistant (Rif(r)) mutants isolates used confirmatory pathogenicity tests. Resistance antibiotic was both stable effective differentiating between an inoculated Rif(r) strain, rifampicin-sensitive contaminants and/or...

The southern green stink bug, Nezara viridula (L.), is a significant pest of cotton, Gossypium hirsutum L., and becoming an increasing challenge due to the decrease in use broad-spectrum insecticides on crop. bug can vector opportunistic Pantoea agglomerans strain (designated Sc 1-R) into cotton bolls, resulting infection. appearance damage varies, managers cannot readily identify its source. This research reports systematic depiction green, immature boll responses at various stages maturity...

Long-held dogma dictates that first instars of Nezara viridula (L.) do not feed, yet recent observations stylet activity within a food source suggest otherwise. As cosmopolitan pest cotton and other high-value cash crops, confirmation feeding by may ultimately influence the knowledge on biology management strategies for this pest. To determine whether newly hatched nymphs were provided sterile green beans (control) or infected with rifampicin-resistant marked bacterial pathogen (Pantoea...

Klebsiella pneumoniae (associated with bacterial pneumonia) was previously isolated from Nezara viridula, a significant vector of cotton boll-rot pathogens. We provide the first annotated genome sequence opportunistic strain K. 5-1. This data provides guidance to study bases pathogenesis by bacteria associated vectors.

Abstract Southern green stink bugs, Nezara viridula (L.) (Hemiptera: Pentatomidae), are pests of cotton recently shown to ingest, retain, and introduce some pathogens into bolls. The objective this study was determine where pathogen colonization occurs in N. after ingestion. Laboratory‐reared adult were fed sterile beans soaked water (control) or previously a suspension one three opportunistic bacterial [ Pantoea agglomerans (Ewing & Fife), ananatis (Serrano) Truper De’Clari, Klebsiella...

Pantoea is comprised of a broad spectrum species, including plant pathogens. Here, we provide an annotated genome sequence sp. strain Sc 1, which was isolated from diseased cotton boll. This research provides the first bona fide insect-vectored pathogen.

The southern green stink bug, Nezara viridula (L.), utilizes stylets while feeding to pierce the wall of a boll cotton, Gossypium hirsutum L., and can inoculate disease-causing pathogens into developing bolls. Detection diseased bolls is difficult because surface carpel frequently lacks apparent macroscopic evidence insect regardless infection; blisters on interior are commonly masked by darkened necrotic tissue. objectives this study were characterize microscopic laboratory-reared bugs...

Cotton (Gossypium hirsutum L.) is a high-value cash crop persistently plagued by insect pests such as stink bugs and related species. Stink vector pathogens that cause necrosis of cotton seed lint, yet the frequency pathogen transmission into successive bolls individual not documented. Individual adults southern green bug, Nezara viridula (L.), (n = 80) were provided beans (Phaseolus vulgaris infected with fungus Eremothecium coryli (Peglion) Kurtzman (syn. Nematospora Peglion) caged...

In 1999, crop consultants scouting for stink bugs (Hemiptera spp.) in South Carolina discovered a formerly unobserved seed rot of cotton that caused yield losses ranging from 10 to 15% certain fields. The disease has subsequently been reported fields throughout the southeastern Cotton Belt. Externally, diseased bolls appeared undamaged; internally, green fruit contain pink dark brown, damp, deformed lint, and necrotic seeds. greenhouse experiments, we demonstrated transmission opportunistic...

Field experiments and supporting laboratory work were conducted to characterize the ability of verde plant bug, Creontiades signatus (Distant), a boll-feeding sucking transmit cotton seed boll rot bacterial pathogen, Serratia marcescens (Bizio) (Enterobacteriales: Enterobacteriaceae). was originally isolated from bolls infested with bug in south Texas, Rifampicin resistant S. strain used transmission retention experiments. Serratia-exposed nonexposed adult bugs colony placed individually on...

To determine the capacity of brown stink bug (BSB) (Euschistus servus) to transmit an infective Pantoea agglomerans strain Sc 1-R into cotton (Gossypium hirsutum) bolls.A laboratory colony BSB was maintained on fresh green beans. Either sterile or contaminated beans were offered adult insects. Strain holds rifampicin resistance (Rif(r) ). Insects then caged with unopened greenhouse-grown bolls. After 2 days, surface sterilized, ground, and plated media without Rif. Two weeks later, seed lint...

Pantoea ananatis is a bacterium with versatile niches that vary from pathogenic to beneficial. We present the genome of strain CFH 7-1, which was recovered diseased greenhouse cotton boll previously caged field-collected fleahopper (Pseudatomoscelis seriatus). These data will assist in deciphering infection process.

A rise in cotton boll rot south Texas has been generally associated with increased yield losses. Here, we measured incidence during two growing seasons (2011 and 2012) at a (Kleberg County) research farm Variety Trial producer fields. The was conducted to compare susceptibility between five current cultivars. commercial fields surveyed were located along the Coastal Bend (Wharton Rio Grande Valley regions (Cameron Willacy Counties). Bolls evidence of external damage potentially inflicted by...

Antibiotic growth promoters that have been historically employed to control pathogens and increase the rate of animal development for human consumption are currently banned in many countries. Probiotics proposed as an alternative pathogenic bacteria. Traditional culture methods typically used monitor probiotic effects on possess significant limitations such a lack sensitivity detect fastidious non-culturable bacteria, both time consuming costly. Here, we tested next generation pyrosequencing...

Southern green stink bug [ Nezara viridula (L.)] adults and other pentatomid pests can transmit pathogens (e.g., the bacterium Pantoea agglomerans ) that cause disease in cotton ( Gossypium hirsutum L.) high-value cash crops worldwide. First instars of N . were recently shown to ingest P strain Sc1R with rifampicin-resistance, retain pathogen 2 nd instar. The objective this study was determine acquisition by early persistence across subsequent stadia. In three trials, (1 st exposed...

Methicillin-resistant Staphylococcus aureus (MRSA) is a Gram-positive bacterium that causes community-acquired and health care-acquired infections. We previously demonstrated clay phyllosilicates customized aluminosilicates display antimicrobial activity against the MRSA strain SQL1. The SQL1 annotated genome reveals USA300 lineage contributes critical knowledge of virulence factors associated with tissue infection.

Pantoea ananatis (Serrano) representatives are known to have a broad host range including both humans and plants. The cotton fleahopper (Pseudatomoscelis seriatus, Reuter) is significant pest that causes bud damage may result in yield losses. In this study, bacterial strain previously isolated from was tested for infectivity using simulated insect feeding. addition, fleahoppers collected the field were raised on green beans laboratory test insects’ capacity vector pathogens. Adult insects...