Response to environmental stresses is a key factor for microbial organism growth. One of the major yeasts in fermentative environments ethanol. Saccharomyces cerevisiae most tolerant species its genus, but intraspecific ethanol-tolerance variation exists. Although, much effort has been done last years discover evolutionary paths improve ethanol tolerance, this phenotype still hardly understood. Here, we selected five strains with different tolerances, and used comparative genomics determine...

This study investigates the impact of sweet potato plant sanitation on yield and external internal quality root storage exploring nutritional content affected by various cooking methods (raw, boiled, oven-cooked). The presence viruses, concretely leaf curl virus (SPLCV), in propagation material is shown to significantly reduce modify quality. Notably, research reveals a substantial improvement crop quality, reinforcing efficacy methods, specifically apical meristem culture, preserving...

Low-temperature growth and fermentation of wine yeast can enhance aroma make them highly desirable traits for the industry. Elucidating response to cold in Saccharomyces cerevisiae is, therefore, paramount importance select or genetically improve new strains. As most enological industrial yeasts, adaptation low temperature is a polygenic trait regulated by many interacting loci.In order unravel genetic determinants low-temperature fermentation, we mapped quantitative loci (QTLs) bulk...

ABSTRACT During batch fermentation, a variety of compounds are synthesized, as microorganisms undergo distinct growth phases: lag, exponential, growth-no-growth transition, stationary, and decay. A detailed understanding the metabolic pathways involved in these phases is crucial for optimizing production target compounds. Dynamic flux balance analysis (dFBA) offers insight into dynamics pathways. However, explaining secondary metabolism remains challenge. multiphase multi-objective dFBA...

ABSTRACT Sterol composition plays a crucial role in determining the ability of yeast cells to withstand high temperatures, an essential trait biotechnology. Using targeted evolution strategy involving fluconazole (FCNZ), inhibitor sterol biosynthesis pathway, and immunosuppressant FK506, we aimed enhance thermotolerance industrial baker's population by modifying their composition. This approach yielded six isolates capable proliferating liquid YPD with μ max values ranging from 0.072 0.236 h...

Interspecific hybridization has played an important role in the evolution of eukaryotic organisms by favouring genetic interchange between divergent lineages to generate new phenotypic diversity involved adaptation environments. This way, Saccharomyces species, involving fusion their metabolic capabilities, is a recurrent adaptive strategy industrial In present study, whole-genome sequences natural hybrids cerevisiae and kudriavzevii were obtained unveil mechanisms origin hybrids, as well...

Yeasts belonging to the Saccharomyces genus play an important role in human-driven fermentations. The species S. cerevisiae has been widely studied because it is dominant yeast most fermentations and used as a model eukaryotic organism. Recently, other of are gaining interest solve new challenges that fermentation industry facing. One these kudriavzevii, which exhibits interesting physiological properties compared cerevisiae, such better adaptation grow at low temperatures, higher glycerol...

Abstract Fungal infections are less studied than viral or bacterial and often more difficult to treat. Saccharomyces cerevisiae is usually identified as an innocuous human-friendly yeast; however, this yeast can be responsible for mainly in immunosuppressed individuals. S. a relevant organism widely used the food industry . Therefore, study of yeasts source clinical infection becoming pivotal question safety. In study, we demonstrate that strains cause spread mostly from environments....

Ancient events of polyploidy have been linked to huge evolutionary leaps in the tree life, while increasing evidence shows that newly established polyploids adaptive advantages certain stress conditions compared their relatives with a lower ploidy. The genus Saccharomyces is good model for studying such events, as it contains an ancient whole-genome duplication event and many sequenced cerevisiae are, speaking, formed polyploids. Many unstable genomes go through large genome erosions;...

ABSTRACT Batch fermentation is a biotechnological dynamic process that produces various products by employing microorganisms undergo different growth phases: lag, exponential, growth-non-growth, stationary, and decay. Genome-scale constrained-based models are commonly used to explore the phenotypic potential of these microorganisms. Previous studies have primarily Flux Balance Analysis (dFBA) elucidate metabolism during exponential phase. However, this approach falls short in addressing...

Numerous plants are traditionally used in their fresh form order to preserve the compounds within and avoid risk of denaturation occurring during drying process [1]. Usually, plant juice is obtained by squeezing freshly harvested plant. Another way make an infusion or alcoholic tincture with For example, oat herb (Avena sativa L.) expressed for relief mild symptoms mental stress aid sleep (EMEA/HMPC/202966/2007). Purple coneflower (Echinacea purpurea (L.) Moench.) also has a well-established...

Abstract Background Evidence suggests that sterol content and composition play an important role in the ability of yeast cells to face high temperatures. Nevertheless, our knowledge exact mechanisms operating is still scarce, which makes rational engineering this industrial-relevant trait difficult. Here, we have used a fluconazole (FCNZ)-driven experimental evolution approach with idea inducing changes biosynthesis pathway linked temperature tolerance. Results The experiment rendered...