A5033139492- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- Photosynthetic Processes and Mechanisms
- Protist diversity and phylogeny
- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- Plant Genetic and Mutation Studies
- Bacterial Genetics and Biotechnology
- RNA Research and Splicing
- Mitochondrial Function and Pathology
- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
Georgia Institute of Technology
2020-2024
Center for Cancer Research
2024
National Institutes of Health
2024
National Cancer Institute
2024
Abundant ribonucleoside-triphosphate (rNTP) incorporation into DNA by polymerases in the form of ribonucleoside monophosphates (rNMPs) is a widespread phenomenon nature, resulting DNA-structural change and genome instability. The rNMP distribution, characteristics, hotspots association with metabolic processes human mitochondrial (hmtDNA) remain mostly unknown. Here, we utilize ribose-seq technique to capture embedded rNMPs hmtDNA six different cell types. In most types, are preferentially...
Ribonucleoside monophosphates (rNMPs) represent the most common non-standard nucleotides found in genome of cells. The distribution rNMPs DNA has been studied only limited genomes. Using ribose-seq protocol and Ribose-Map bioinformatics toolkit, we reveal incorporated into whole a photosynthetic unicellular green alga, Chlamydomonas reinhardtii. We discovered disproportionate incorporation adenosine mitochondrial chloroplast DNA, contrast to nuclear relative corresponding nucleotide content...
Abstract We aim to offer new insights on the regulation of metabolic plasticity in renal cell carcinoma (RCC) and identify novel regulatory targets that can be used for potential treatment therapies disease. RCC affects an estimated 400,000 people worldwide each year with over 100,00 deaths annually disproportionately racial/ethnic minorities United States. is a term covers array kidney cancer subtypes differing incidence, histopathology, genetic molecular alterations, as well clinical...
Summary Ribonucleoside monophosphates (rNMPs) represent the most common non-standard nucleotides found in genomic DNA of cells. The distribution rNMPs has been studied only limited genomes, such as yeast nuclear and mitochondrial DNA, well human DNA. In this study, we used ribose-seq protocol Ribose-Map bioinformatics toolkit to reveal incorporated into whole genome a photosynthetic unicellular green alga, Chlamydomonas reinhardtii . study presents discovery disproportionate incorporation...
Double-strand breaks (DSBs) in DNA are naturally occurring destructive events all organisms that may lead to genome instability. Cells employ various repair methods known as non-homologous end joining (NHEJ), microhomology mediated (MMEJ), and homology-directed recombination (HDR). These processes sequence variations (e.g., nucleotide insertions, deletions, substitutions) at the location of break. Studying DSB often involves use high throughput sequencing assays precisely quantify near break...
Summary Double-strand breaks (DSBs) in DNA are challenging lesions to repair. Human cells employ at least three DSB repair mechanisms, with a preference for non-homologous end joining (NHEJ) over homologous recombination (HR) and microhomology-mediated (MMEJ) 1,2 . In contrast HR, NHEJ MMEJ do not utilize template molecule recover damaged and/or lost nucleotides 2 directly ligates broken ends, while exploits the alignment of short microhomologies on sides is associated deletions sequence...