A5024904339- Intraocular Surgery and Lenses
- Connexins and lens biology
- Corneal surgery and disorders
- Ophthalmology and Visual Impairment Studies
- Corneal Surgery and Treatments
- Ocular Surface and Contact Lens
- Ocular Infections and Treatments
- Urinary Bladder and Prostate Research
- Urological Disorders and Treatments
- Ocular Disorders and Treatments
- Pituitary Gland Disorders and Treatments
- Amyloidosis: Diagnosis, Treatment, Outcomes
- CRISPR and Genetic Engineering
- Tribology and Lubrication Engineering
- Surgical Sutures and Adhesives
- Spectroscopy Techniques in Biomedical and Chemical Research
- Anorectal Disease Treatments and Outcomes
- Tissue Engineering and Regenerative Medicine
- Neuroscience and Neural Engineering
- Retinal Development and Disorders
- Myasthenia Gravis and Thymoma
Universitat Autònoma de Barcelona
2015-2024
Barraquer Opthalmology Center
2015-2024
Instituto Barraquer de América
2020
Bacterial nanocellulose exhibits valuable properties to act as a corneal bandage material in terms of conformability, suturability, durability and ease manipulation ophthalmological environments.
<b><i>Purpose:</i></b> To evaluate a new nuclear cataract grading system which is intended as surgical guidance to predict lens hardness before surgery. <b><i>Methods:</i></b> The BCN 10 consists of frontal and cross-sectional slit-lamp images human eye lenses, ranging from completely transparent nucleus totally black cataract. Validation was done with 9 observers for 110 cases. Two modalities were applied, asked use only whole digits then half...
BackgroundVisual impairments are a critical medical hurdle to be addressed in modern society. Müller glia (MG) have regenerative potential the retina lower vertebrates, but not mammals. However, mice, vivo cell fusion between MG and adult stem cells forms hybrids that can partially regenerate ablated neurons.MethodsWe used organotypic cultures of human preparations dissociated test hypothesis induce neuronal regeneration systems. Moreover, we established microinjection system for...
In order to determine whether posterior capsule opacification after cataract surgery, could be delayed or inhibited through the application of hydrogen peroxide (H2O2) distilled water (H2Od),we extracted lens capsules from 25 human donor eye globes. Samples were treated for 5 min with either 30 mM H2O2 H2Od used as controls, and cultured one month, during which dark field tilt illumination photos taken. These observe quantify, time until cellular growth confluence on capsule. After culture,...
To explore alternative experimental protocols to investigate the biomechanical behavior of crystalline lens and zonules using ex vivo stretching.Radial stretching tests were conducted on anterior segment (consisting lens, zonules, ciliary body, sclera) four pairs presbyopic human donor eyes. A simple mechanical model is used describe when tested in this way. Each pair samples was initially stretched with body intact. One sample retested after cutting radially, other removing lens.The...
Purpose: Human cortical opacities are most commonly accompanied by changes in lens fiber structure the equatorial region at nucleus–cortex interface. Cortex and nucleus have different elastic properties, which change with age. We therefore subjected ex vivo lenses to simulated accommodation studied internal deformations better understand mechanism of cataract formation. Methods: Nine human donor (33–88 years old) were tested using a bespoke radial stretching device for anterior eye segments....
Soemmerring's rings consist of a ring lens epithelial derived cells that grow along the periphery an aphakic capsule, or around intraocular lens. These when visualized frontally, appear opaque, however, in some cases compose these are organized same fashion as those normal transparent adult lenses. Thus, our purpose was to test whether any part could be and how this related morphological factors. To study this, 16 were extracted from donor eye globes. After imaging, they thickly sectioned...
Purpose: To test whether any part of Soemmerring's rings developed, in‐vivo, in adults could be transparent and how this transparency relates to morphological factors. Methods: Sixteen were extracted from donor eye globes. After frontal imaging, they thickly sectioned sagittally order analyse the degrees cross‐section both at periphery center. All samples also histologically analysed using alpha smooth muscle actin, Vimentin, wheat germ agglutinin DAPI correlate certain Results: When viewed...
The ability of the normal human eye to accommodate declines steadily with increasing age. This has consequence that – beyond an age about 50 or 60 years amplitude accommodation effectively zero. It is widely considered principal cause this decline age-related increase in lens stiffness. process driven by radial movements ciliary body; these variations forces applied via zonules. In young (pre-presbyopic) force deform consequential adjustments optical power eye. older (presbyopic eyes) it...
Purpose To evaluate the performance of a manual lens stretching device using clinical scanners to analyze variations in topography and cross sections. Methods The consists 6 pulling elements, attached an external ring, which act like aperture. anterior segment enucleated eyes from six months old pigs were glued device, then cornea posterior eye removed; finally, whole setup was placed inside container, controls process. Different (Casia OCT, Orbscan Pentacam) used shape. Calibrations...
Summary In previous publications, we found that human cortical and cuneiform opacities are accompanied by changes in fiber structure architecture mainly the equatorial border zone between lens nucleus cortex. Because cortex have different viscoelastic properties young old lenses, hypothesized external forces during accommodation cause shear stress predominantly at this nucleus‐cortex interface. The location of described suggested these mechanical may disorganization, small opacities,...
Purpose To observe the reactions of lens epithelial cells (LECs) to a short exposure an elevated oxidative stress. help find future treatments for prevention posterior capsule opacification. Using cultured human capsules as experimental model and hydrogen peroxide (H2O2) stress inducer. Methods Lens capsule-ciliary body complexes were extracted from donor eyes. Samples exposed 30 mM H2O2 5 min (n = 8) or used untreated controls 9). was applied after extraction through intercapsular...
Abstract Purpose To compare bacterial nanocellulose (BNC) patches produced by K.xylinus, against the amniotic membrane (AM) as a bandage material for ocular surface disorders. Based on previous structural characterizations of our BNC patches, and detailed cytocompatibility tests with human dermal fibroblasts; we hypothesise that is viable corneal Methods We tested mechanical resistance ( n = 6) AM 5) samples using progressively weighted sutures to tear samples. In order test stability, 6...