A5020091525- Microfluidic and Capillary Electrophoresis Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Microfluidic and Bio-sensing Technologies
- Advanced Biosensing Techniques and Applications
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Electrowetting and Microfluidic Technologies
- Molecular Biology Techniques and Applications
- Mosquito-borne diseases and control
- Gene expression and cancer classification
- Microbial Community Ecology and Physiology
- Nanopore and Nanochannel Transport Studies
- SARS-CoV-2 detection and testing
- Data-Driven Disease Surveillance
- Protein purification and stability
- Antibiotic Resistance in Bacteria
- Atmospheric Ozone and Climate
- Lipid Membrane Structure and Behavior
- Atmospheric chemistry and aerosols
- Mycobacterium research and diagnosis
- Cell Image Analysis Techniques
- CRISPR and Genetic Engineering
- Water Quality Monitoring and Analysis
Sandia National Laboratories California
2014-2024
Sandia National Laboratories
2010-2019
Indiana University – Purdue University Indianapolis
2008-2019
Lawrence Livermore National Security
2014-2019
Bioengineering Center
2010-2018
10X Genomics (United States)
2016
Joint BioEnergy Institute
2014
Northwestern University
2002-2011
National Technical Information Service
2010
Office of Scientific and Technical Information
2010
Exposure of therapeutic and diagnostic medical devices to biological fluids is often accompanied by interfacial adsorption proteins, cells, microorganisms. Biofouling surfaces can lead compromised device performance or increased cost in some cases may be life-threatening the patient. Although numerous antifouling polymer coatings have enjoyed short-term success preventing protein cell on surfaces, none proven ideal for conferring long-term biofouling resistance. Here we describe a new...
Current multiplexed diagnostics for Zika, dengue, and chikungunya viruses are situated outside the intersection of affordability, high performance, suitability use at point-of-care in resource-limited settings. Consequently, insufficient diagnostic capabilities a key limitation facing current Zika outbreak management strategies. Here we demonstrate highly sensitive specific detection chikungunya, dengue by coupling reverse-transcription loop-mediated isothermal amplification (RT-LAMP) with...
In the oral cavity, chronic inflammation has been observed at various stages of squamous cell carcinomas (OSCC). Such could result from persistent mucosal or epithelial colonization by microorganisms. There is increasing evidence involvement bacteria in inflammation, warranting further studies on association with progression OSCC. The objective this study was to evaluate diversity and relative abundance saliva subjects Using 454 parallel DNA sequencing, ∼58,000 PCR amplicons that span V4-V5...
Multidrug-resistant Enterobacteriaceae are emerging as a serious infectious disease challenge. These strains can accumulate many antibiotic resistance genes though horizontal transfer of genetic elements, those for β-lactamases being particular concern. Some active on broad spectrum β-lactams including the last-resort carbapenems. The gene broad-spectrum and carbapenem-active metallo-β-lactamase NDM-1 is rapidly spreading. We present complete genome Klebsiella pneumoniae ATCC BAA-2146, first...
Reverse-transcription-loop-mediated isothermal amplification (RT-LAMP) has frequently been proposed as an enabling technology for simplified diagnostic tests RNA viruses. However, common detection techniques used LAMP and RT-LAMP have drawbacks, including poor discrimination capability, inability to multiplex targets, high rates of false positives, (in some cases) the requirement opening reaction tubes postamplification. Here, we present a simple technique that allows closed-tube,...
Bumping the priming sites for RT-LAMP based on thermodynamic predictions can dramatically reduce non-specific background amplification.
Next-generation sequencing (NGS) is emerging as a powerful tool for elucidating genetic information wide range of applications. Unfortunately, the surging popularity NGS has not yet been accompanied by an improvement in automated techniques preparing formatted libraries. To address this challenge, we have developed prototype microfluidic system sequencer-ready DNA libraries analysis Illumina sequencing. Our combines droplet-based digital (DMF) sample handling with peripheral modules to...
Towards designing a portable diagnostic device for detecting biological toxins in bodily fluids, we have developed microfluidic chip-based immunoassays that are rapid (< 20 minutes), require minimal sample volume (<10 µL) and appreciable sensitivity dynamic range (µM–pM). The chip is being integrated with miniaturized electronics, optical elements, fluid-handling components, data acquisition software to develop portable, self-contained device. intended rapid, point-of-care (and, future,...
Smartphones have shown promise as an enabling technology for portable and distributed point-of-care diagnostic tests. The CMOS camera sensor can be used detecting optical signals, including fluorescence applications such isothermal nucleic acid amplification However, analysis is typically limited mostly to end point detection of single targets. Here we present a smartphone-based image pipeline that utilizes the CIE xyY (chromaticity-luminance) color space measure luminance (in lieu RGB...
High-throughput screening assays of native and recombinant proteins are increasingly crucial in life science research, including fields such as drug enzyme engineering. These typically highly parallel, require minute amounts purified protein per assay. To address this need, we have developed a rapid, automated microscale process for isolating specific from sub-microlitre volumes E. Colicell lysate. Recombinant genetically tagged to drive partitioning into the PEG-rich phase flowing aqueous...
Technologies that allow for high-throughput, economical, and accurate single nucleotide polymorphism (SNP) genotyping are becoming crucial modern genomic efforts. Here, we present a method multiplexed single-base extension (SBE) takes advantage of the unique separation modalities made possible via end-labeled free-solution electrophoresis (ELFSE). Three SBE oligonucleotide primers, which probe mutations clinical importance in human p53 gene, were covalently conjugated to three polypeptoid...
Using relative rate techniques the reactions of chlorine and fluorine atoms with HC(O)F have been determined to proceed constants k1 = (1.9 ± 0.2) × 10−15 k2 (8.3 1.7) 10−13 cm3 molecule−1 s−1, respectively. Stated errors reflect statistical uncertainty; possible systematic uncertainties could add additional 10% 20% ranges values k2, Experiments were performed at 295 2 K 700 torr total pressure air. The results are discussed respect design interpretation laboratory studies atmospheric...
We describe an integrated microfluidic device (μFlowFISH) capable of performing 16S rRNA fluorescence in situ hybridization (FISH) followed by flow cytometric detection for identifying bacteria natural microbial communities. The was used species involved bioremediation Cr(VI) and other metals groundwater samples from a highly-contaminated environmental site (Hanford, WA, USA). μFlowFISH seamlessly integrates two components: chamber formed between photopolymerized membranes, where cells...
Digital microfluidics (DMF) is a powerful technique for sample preparation and analysis broad range of biological chemical applications.
Multiple displacement amplification (MDA) is a widely used technique for of DNA from samples containing limited amounts (e.g., uncultivable microbes or clinical samples) before whole genome sequencing. Despite its advantages high yield and fidelity, it suffers bias non-specific when amplifying sub-nanogram template DNA. Here, we present microfluidic digital droplet MDA (ddMDA) where partitioning the into thousands sub-nanoliter droplets, each small number fragments, greatly reduces...
Our novel pressure balancing structure stabilizes a picoinjector, allowing reagent injection into flowing droplets in channels with fluctuating pressure.
Staphylococcus aureus is a major human pathogen of the skin. The global burden diabetes high, with S. being complication diabetic wound infections. We investigated how environment influences skin infection and observed an increased susceptibility to in mouse models both type I II diabetes. A dual gene expression approach was taken investigate transcriptional alterations host bacterium after infection. While analysis response revealed only minor changes between infected control mice, we that...
Isothermal titration microcalorimetry has been used to measure enthalpies of aggregate formation and binding, at 25 °C, for aqueous solutions the single surfactants sodium dodecyl sulfate (SDS) dodecyltrimethylammoniumbromide (DTAB) mixtures these 1 wt % total surfactant concentration, over accessible composition ranges. The enthalpy SDS micelles is 0.6 kJ g-1 mol-1, that DTAB −2.1 mol-1. This calorimetric experiment also produces a direct measurement critical micelle concentration (cmc)...
Collection of mosquitoes and testing for vector-borne viruses is a key surveillance activity that directly influences the vector control efforts public health agencies, including determining when where to apply insecticides. Vector districts in California routinely monitor three human pathogenic West Nile virus (WNV), Western equine encephalitis (WEEV), St. Louis (SLEV). Reverse transcription quantitative polymerase chain reaction (RT-qPCR) offers highly sensitive specific detection these...
Combined activity- and immunoassays for CRISPR/Cas9 on a portable microfluidic device with integrated sample preparation from clinical matrices.