A5033302964- FOXO transcription factor regulation
- Fungal and yeast genetics research
- MicroRNA in disease regulation
- Autophagy in Disease and Therapy
- Mitochondrial Function and Pathology
- Magnesium in Health and Disease
- Metabolism, Diabetes, and Cancer
- CRISPR and Genetic Engineering
- Circular RNAs in diseases
- Genomic variations and chromosomal abnormalities
- Mast cells and histamine
- Plant nutrient uptake and metabolism
- Mycotoxins in Agriculture and Food
- Ovarian function and disorders
- Beetle Biology and Toxicology Studies
- Genetics, Aging, and Longevity in Model Organisms
- PARP inhibition in cancer therapy
- Endodontics and Root Canal Treatments
- Plant Stress Responses and Tolerance
- Cutaneous Melanoma Detection and Management
- Aluminum toxicity and tolerance in plants and animals
- Ocular Oncology and Treatments
- Redox biology and oxidative stress
- Dental Trauma and Treatments
- Chromosomal and Genetic Variations
Istanbul University
2005-2024
Istanbul Yeni Yüzyıl University
2018-2022
Sabancı Üniversitesi
2016
KU Leuven
2011-2015
Catholic University of America
2011
Glioblastoma multiforme (GBM) is a life-threatening brain tumor. Accumulating evidence suggests that eradication of glioma stem-like cells (GSCs) in GBM essential to achieve cure. The transcription factor FOXM1 has recently gained attention as master regulator mitotic progression cancer various organs. Here, we demonstrate forms protein complex with the kinase MELK GSCs, leading phosphorylation and activation kinase-dependent manner. This MELK-dependent results subsequent increase regulatory...
Maternal embryonic leucine zipper kinase (MELK), a serine/threonine protein kinase, has oncogenic properties and is overexpressed in many cancer cells. The function of MELK attributed to its capacity disable critical cell-cycle checkpoints reduce replication stress. Most functional studies have relied on the use siRNA/shRNA-mediated gene silencing. In present study, we explored biological using MELK-T1, novel selective small-molecule inhibitor. Strikingly, MELK-T1 triggered rapid...
Autophagy is biological mechanism allowing recycling of long-lived proteins, abnormal protein aggregates, and damaged organelles under cellular stress conditions. Following sequestration in double- or multimembrane autophagic vesicles, the cargo delivered to lysosomes for degradation. ATG5 a key component an E3-like ATG12-ATG5-ATG16 complex that catalyzes conjugation MAP1LC3 lipids, thus controlling vesicle formation expansion. Accumulating data indicate convergence point autophagy...
Maternal embryonic leucine zipper kinase (MELK) belongs to the subfamily of AMP-activated Ser/Thr protein kinases. The expression MELK is very high in glioblastoma-type brain tumors, but it not clear how this contributes tumor growth. Here we show that siRNA-mediated loss U87 MG glioblastoma cells causes a G1/S phase cell cycle arrest accompanied by death or senescence-like phenotype can be rescued siRNA-resistant MELK. This mediated an increased p21(WAF1/CIP1), inhibitor cyclin-dependent...
We have isolated 14 different Schizosaccharomyces pombe mutants that synthesize invertase enzyme constitutively. Analyses of activities revealed the degrees resistance to glucose repression were not similar among complementation groups. One groups appeared be associated with functional and/or regulatory defects in hexose transport. Another group specific for regulation inv1 gene alone, implying these mutations might genes acting on sensing and signaling pathway. In addition, we found...
Objectives: Dehydroepiandrosterone (DHEA) is an endogenous hormone that acts as a ligand for several cellular receptors.An age-dependent decline in circulating levels of DHEA linked to changes various physiological functions.In gynecological clinical practice, commonly prescribed induce ovulation.Some studies report positive association between high serum concentrations and increased risk developing ovarian cancer.However, the vitro effects on cancerous cells have not been explored thus...
We aimed to investigate the in vitro physiologic effects of xylene, chloroform, orange oil and eucalyptus solvents for dissolving gutta-percha on L929 HOB cell lines; 2.5 10 μL mL-1 these were tested 24, 48 72 h. Gutta-percha inhibited proliferation rate fibroblasts a dose- time-dependent manner; however, no inhibition was detected (evaluated using MTT assay). None induced apoptosis/necrosis cells at ≤2.5 concentration contrast (determined acridine orange/ethidium bromide dual staining)....
Metformin, a widely used first-line medication in the treatment of type II diabetes, has been proposed to have second indication cancers and aging. However, its accounting mechanisms cellular physiology were not clearly understood. Therefore, cytotoxicity underlying physiological should be explained. Schizosaccharomyces pombe was evaluated as single-cell model treated with metformin grown on YEL media at 30 °C 180 rpm. 0,1-20 mM caused dose-dependent apoptosis necrosis demonstrated by using...
Objectives: Betahistine is a histamine analog commonly prescribed for symptomatic treatment of vertiginous symptoms.In vitro studies have shown that betahistine was not toxic at the doses in nasal epithelial cell line.However, effect on other types has been studied.In this study, we aimed to investigate some physiological effects L929 fibroblast, A549 lung cancer, human umbilical vein endothelial (HUVEC), and Ishikawa endometrial lines.Materials Methods: Cellular proliferation assed assessed...
Abstract Maternal embryonic leucine zipper kinase (MELK, hMP38, pEG3), a Ser/Thr protein kinase, is highly overexpressed in stem cells and cancer cells. The oncogenic role of MELK attributed to disabling critical cell cycle check points as well enhancing replication. Most functional studies have relied on the use siRNA/shRNA mediated gene silencing, but this often can be associated with off-target effects. Here we want present novel, potent selective small molecule inhibitor JNJ-47117096...
Humans are unknowingly exposed to mycotoxins through the consumption of plant-derived foods and processed products contaminated with these toxic compounds. In addition agricultural losses, Fusarium toxins pose a threat human health. However, effects fusariotoxins on viability proliferation stem cells have not been fully explored. We investigated cytotoxic deoxynivalenol (DON) B-trichothecene mix (MIX) mesenchymal (MSCs) L929 fibroblast cell line.
Objective: Inter-retrotransposon polymorphism Polymerase Chain Reaction (IRAP-PCR) technique allows for detecting insertional polymorphisms via amplification of the DNA fragment between two retrotransposons in plant genomes. However, this method has not been reported to be used analyzing human samples date. Recently, Human Endogenous Retrovirus (HERV) gained interest due their potential effect on pathophysiology certain diseases. Nevertheless, association HERV and risk developing nasal...
Magnezyum, enerji metabolizması, nükleik asit ve protein sentezi, sinyal iletimi, hücre bölünmesi gibi birçok biyolojik süreç için hayati önem taşır. Magnezyum homeostasisinin bozulması, kardiyovasküler hastalıklar, hipertansiyon, tip 2 diyabet kanser başta olmak üzere çok sayıda hastalıkla ilişkilendirilmiştir. Dünya çapında 300 milyondan fazla insan ile mücadele etmektedir bu sayı katlanarak artmaktadır. Klinik çalışmalar, diyabetli hastalarda serum magnezyum seviyesinin düştüğünü...