- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- Agronomic Practices and Intercropping Systems
- Plant and soil sciences
- Plant Micronutrient Interactions and Effects
- Plant Molecular Biology Research
- Aluminum toxicity and tolerance in plants and animals
- Plant responses to water stress
- Seed and Plant Biochemistry
- Plant-Microbe Interactions and Immunity
- Seed Germination and Physiology
- Coastal wetland ecosystem dynamics
- Wastewater Treatment and Nitrogen Removal
- Microbial Metabolites in Food Biotechnology
- Soybean genetics and cultivation
- Single-cell and spatial transcriptomics
- Nematode management and characterization studies
- Photosynthetic Processes and Mechanisms
- Trace Elements in Health
- Plant and fungal interactions
- Biochemical Acid Research Studies
- Allelopathy and phytotoxic interactions
- Aquaculture Nutrition and Growth
- Plant Reproductive Biology
Centro de Recursos Educativos Avanzados
2018-2025
Consejo Superior de Investigaciones Científicas
2008-2021
Instituto de Ciencias Agrarias
2012-2021
Instituto de Estudios Avanzados
2020
Universidad Católica del Norte
2016-2018
Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas
2001-2006
Institut des Sciences du Végétal
1994-1997
Universidade de Vigo
1996-1997
Zero to Three
1997
Centre National de la Recherche Scientifique
1994-1996
Abstract A Gram‐positive, fast‐growing, endophytic bacterium was isolated from root nodules of Medicago polymorpha and identified as Bacillus megaterium . The isolate, named NMp082, co‐inhabited with the symbiotic rhizobium Ensifer medicae B. NMp082 contained nifH nodD genes that were 100% identical to those meliloti , an unusual event suggested previous lateral gene transfer a different rhizobial species. Despite presence nodulation nitrogen fixation genes, endophyte not able form effective...
ABSTRACT Legume root nodule nitrogen‐fixing activity is severely affected by osmotic stress. Proline accumulation has been shown to induce tolerance salt stress, and transgenic plants over‐expressing Δ 1 ‐pyrroline‐5‐carboxylate synthetase (P5CS), which accumulates high levels of proline, display enhanced osmotolerance. Here, we transformed the model legume Medicago truncatula with P5CS gene from Vigna aconitifolia , was evaluated under stress in that showed proline levels. Nitrogen fixation...
Abstract Increasing soil salinity represents a major constraint for agriculture in arid and semi‐arid lands, where mineral nitrogen (N) deficiency is also frequent characteristic of soils. Biological N fixation by legumes may constitute sustainable alternative to chemical fertilisation salinity‐affected areas, provided that adapted cultivars inoculants are available. Here, the performance three peanut nodulated with two different rhizobial strains differ their salt tolerance was evaluated...
Several recent studies have demonstrated that the expression of a cyanobacterial flavodoxin in plants can provide tolerance to wide range environmental stresses. Indeed, this strategy has been proposed as potentially powerful biotechnological tool generate multiple-tolerant crops. To determine whether specifically increased salt stress and it might also preserve legume nitrogen fixation under saline conditions, gene was introduced into model Medicago truncatula. Expression did not confer...
ABSTRACT Legumes are among the most utilised agronomic plant species due to their symbiotic association with N 2 ‐fixing bacteria. Since fixation entails high ATP cost, salt stress disrupts in symbiont, but increases production of osmolytes and antioxidant systems host plant. This results competition for C allocation between osmoprotection continued supply symbiont acquisition, which may result different responses salinity. Two‐nodule types contrasting carbon requirements organic can be...
In the north and center of Chile, there has been a significant environmental contamination by copper due to natural factors, mining activities use agricultural fertilizers pesticides. Copper (Cu) soil is important concern in agriculture, food safety, human health. Soil concentrations higher than 100–150 mg/kg can be toxic for plants other organisms. Therefore, identification copper-tolerant crops great interest sustainable cultivation purposes. Quinoa promising candidate as crop, owing its...
Summary Under nitrogen starvation, Rhizobium meliloti is able to induce nitrogen‐fixing nodules on alfalfa roots. Certain cultivars spontaneously develop pseudonodules in the absence of bacteria. A transcript, Msca1 , expressed spontaneous and R. ‐induced nodules, that codes for a carbonic anhydrase (CA), an enzyme catalyzing hydration CO 2 has been identified. This first CA gene cloned from non‐photosynthetic tissue plants. was activated initially all cells bacterium‐induced nodule...
Abstract Sinorhizobium meliloti cells were engineered to overexpress Anabaena variabilis flavodoxin, a protein that is involved in the response oxidative stress. Nodule natural senescence was characterized alfalfa (Medicago sativa) plants nodulated by flavodoxin-overexpressing rhizobia or corresponding control bacteria. The decline of nitrogenase activity and nodule structural ultrastructural alterations are associated with significantly delayed flavodoxin-expressing nodules. Substantial...
Denitrification is defined as the dissimilatory reduction of nitrate or nitrite to nitric oxide (NO), nitrous (N2O), dinitrogen gas (N2). N2O a powerful atmospheric greenhouse and cause ozone layer depletion. Legume crops might contribute production by providing nitrogen-rich residues for decomposition associating with rhizobia that are able denitrify under free-living symbiotic conditions. However, there limited direct empirical data concerning endosymbiotic bacteria associated legume...
Mercury (Hg) is extremely toxic for all living organisms. Hg-tolerant symbiotic rhizobia have the potential to increase legume tolerance, and our knowledge, mechanisms underlying Hg tolerance in not been investigated date. Rhizobial strains of Ensifer medicae , Rhizobium leguminosarum bv. trifolii Bradyrhizobium canariense previously isolated from severely Hg-contaminated soils showed different levels tolerance. The ability reduce mercury 2+ 0 a volatile less form mercury, was assessed using...
Cadmium (Cd) accumulation in agricultural soils constitutes a serious problem for crop yields and food safety. It is known that proline (Pro) can rapidly accumulate plant tissues response to abiotic stress. To analyze the potential protective effect of Pro against Cd toxicity, we compared stress wild-type (WT) Medicago truncatula transgenic line had previously obtained characterized (p18), which expressed Δ 1-pyrroline-5-carboxylate synthetase gene from Vigna aconitifolia (VaP5CS),...
Lotus japonicus determinate nodules differ greatly from indeterminate in their organogenesis and morphological characteristics, whereas Lupinus albus lupinoid share features of nodules. The mitotic inhibitor Ccs52A is essential for endoreduplication ploidy-dependent cell enlargement during symbiotic differentiation Medicago truncatula ccs52A homolog genes were isolated lupin lotus nodules; the deduced proteins showed high sequence similarity with other Cdh-1-type activators...