A5002449664- Nanopore and Nanochannel Transport Studies
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Lipid Membrane Structure and Behavior
- Bacteriophages and microbial interactions
- CRISPR and Genetic Engineering
- Microfluidic and Capillary Electrophoresis Applications
- Mechanical and Optical Resonators
- Photosynthetic Processes and Mechanisms
- Microfluidic and Bio-sensing Technologies
- Photoreceptor and optogenetics research
- Polysaccharides and Plant Cell Walls
- Electrochemical Analysis and Applications
- Plant Surface Properties and Treatments
- Ion-surface interactions and analysis
- bioluminescence and chemiluminescence research
- Plant Reproductive Biology
Technical University of Darmstadt
2016-2024
Sanofi (Germany)
2023
Sanofi (France)
2022
A nanopore-based CuII -sensing system is reported that allows for an ultrasensitive and selective detection of with the possibility a broad range applications, example in medical diagnostics. fluorescent ATCUN-like peptide 5/6-FAM-Dap-β-Ala-His employed to selectively bind ions presence NiII ZnII was crafted into ion track-etched nanopores. Upon binding fluorescence sensor quenched, permitting solution. The transport characteristics peptide-modified nanopore are shown be extremely sensitive...
The development of flexible and reconfigurable sensors that can be readily tailored toward different molecular analytes constitutes a key goal formidable challenge in biosensing. In this regard, synthetic nanopores have emerged as potent physical transducers to convert interactions into electrical signals. Yet, systematic strategies functionalize their surfaces with receptor proteins for the selective detection remain scarce. Addressing these limitations, general strategy is presented...
A broad variety of biomolecules is industrially produced in bacteria and yeasts. These microbial expression hosts can be optimized through genetic engineering using CRISPR tools. Here, we designed characterized such a modular genome editing system based on the Cas12a-like RNA-guided nuclease MAD7 Escherichia coli. This enables efficient generation single nucleotide polymorphisms (SNPs) or gene deletions directly used with donor DNA from benchtop assembly to increase throughput. We combined...
Nanopores comprise a versatile class of membrane proteins that carry out range key physiological functions and are increasingly developed for different biotechnological applications. Yet, capacity to study engineer protein nanopores by combinatorial means has so far been hampered lack suitable assays combine sufficient experimental resolution with throughput. Addressing this technological gap, the functional nanopore (FuN) screen now provides quantitative dynamic readout assembly function in...
Membranes provide a highly selective barrier that defines the boundaries of any cell while providing an interface for communication and nutrient uptake. However, despite their central physiological role, our capacity to study or even engineer permeation distinct solutes across biological membranes remains rudimentary. This especially applies Gram-negative bacteria, where outer inner membrane impose two barriers. Addressing this analytical challenge, we exemplify how permeability Escherichia...
The functional analysis of protein nanopores is typically conducted in planar lipid bilayers or liposomes exploiting high-resolution but low-throughput electrical and optical read-outs. Yet, the reconstitution vitro still constitutes an empiric process. Addressing these limitations, can now be analyzed using nanopore (FuN) screen genetically encoded fluorescent sensors that resolve distinct nanopore-dependent Ca2+ in- efflux patterns across inner membrane Escherichia coli. With a primary...
Planar lipid bilayers constitute a versatile method for measuring the activity of protein channels and pores on single molecule level. Ongoing efforts attempt to tailor this detecting biomedically relevant target analytes or high-throughput screening drugs. To improve mechanical stability bilayer recordings, we use thin-film epoxy resist ADEX as septum in free-standing vertical bilayers. Defined apertures with diameters between 30 µm 100 were micro-fabricated by photolithography. The...
Abstract A broad variety of biomolecules is industrially produced in bacteria and yeasts. These microbial expression hosts can be optimized through genomic engineering using CRISPR tools. Here, we designed characterized such a modular genome editing system based on the Cas12a-like RNA guided nuclease MAD7 E. coli . Our enables efficient generation single nucleotide polymorphisms (SNPs) or gene deletions directly used with donor DNA from benchtop assembly to increase throughput. We combined...
Abstract The functional analysis of protein nanopores is typically conducted in planar lipid bilayers or liposomes exploiting high-resolution but low-throughput electrical and optical read-outs. Yet, the reconstitution vitro still constitutes an empiric process. Addressing these limitations, can now be analyzed using nanopore (FuN) screen genetically-encoded fluorescent sensors that resolve distinct nanopore-dependent Ca 2+ in- efflux patterns across inner membrane Escherichia coli . With a...
Abstract Nanopores constitute an important class of biotechnologically relevant proteins. Unlike binders and enzymes, their experimental characterization is limited to high-resolution, yet low throughput biophysical methods. Addressing this technological gap, the functional nanopore (FuN) screen now provides a versatile assay study engineer nanopores in Escherichia coli combining quantitative resolution with ease, scalability, cellular assays.
Abstract Nanopores comprise a versatile class of membrane proteins that carry out range key physiological functions and are increasingly developed for different biotechnological applications. Yet, capacity to study engineer protein nanopores by combinatorial means has so far been hampered lack suitable assays combine sufficient experimental resolution with throughput. Addressing this technological gap, the Functional Nanopore (FuN) screen now provides quantitative dynamic read-out nanopore...